##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AccIII AclI AflIII Aor51HI AscI
AseI AsnI AviII AvrII BbeI
BbrPI BciVI BlnI BlpI Bpu1102I
BsaBI BseAI BsiWI BspDI BspEI
BspLU11I BsrBI BssHII Bst1107I BstBI
BstZ17I CelII ClaI Eco47III EcoT22I
FseI FspI KasI KspI MamI
MfeI MluI MroI MunI NarI
NotI NruI NsiI NspV PacI
PaeR7I PmaCI PmeI PmlI PshBI
Psp1406I PvuI SacII SbfI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 1834 GACGTC GACGT^C
Acc65I 3707 GGTACC G^GTACC
AgeI 2088 ACCGGT A^CCGGT
AhdI 5235 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 3707 GGTACC G^GTACC
AspEI 5235 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 5045 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1280 CCCGGG C^[CT]CG[GA]G
BamHI 2186 GGATCC G^GATCC
BbvCI 66 CCTCAGC CCTCAGC(-5/-2)
BcgI 1194 CGAGGCCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 4552 AGATCT A^GATCT
BsaAI 1057 CACGTA [CT]AC^GT[GA]
BsaWI 2088 ACCGGT [AT]^CCGG[AT]
BsmI 2923 GCATTC GAATGC(1/-1)
BsoBI 1280 CCCGGG C^[CT]CG[GA]G
BspHI 4079 TCATGA T^CATGA
BstEII 5128 GGTGACC G^GT[ACGT]ACC
BstPI 5128 GGTGACC G^GT[ACGT]ACC
CpoI 878 CGGACCG CG^G[AT]CCG
EagI 1216 CGGCCG C^GGCCG
Eam1105I 5235 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1216 CGGCCG C^GGCCG
Eco52I 1216 CGGCCG C^GGCCG
EcoO65I 5128 GGTGACC G^GT[ACGT]ACC
HindIII 2881 AAGCTT A^AGCTT
HpaI 61 GTTAAC GTT^AAC
KpnI 3707 GGTACC GGTAC^C
NheI 4546 GCTAGC G^CTAGC
NspI 413 ACATGC [GA]CATG^[CT]
PflFI 5045 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2088 ACCGGT A^CCGGT
RcaI 4079 TCATGA T^CATGA
RsrII 878 CGGACCG CG^G[AT]CCG
SalI 1837 GTCGAC G^TCGAC
SapI 4222 GCTCTTC GCTCTTC(1/4)
ScaI 283 AGTACT AGT^ACT
SexAI 350 ACCAGGT A^CC[AT]GGT
SmaI 1280 CCCGGG CCC^GGG
StuI 1196 AGGCCT AGG^CCT
Tth111I 5045 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1280 CCCGGG C^CCGGG
XmaI 1280 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflII 3497 CTTAAG C^TTAAG
5551 CTTAAG
AocI 4206 CCTTAGG CC^T[ACGT]AGG
4477 CCTGAGG
BbsI 2281 GTCTTC GAAGAC(2/6)
3447 GTCTTC
BfrI 3497 CTTAAG C^TTAAG
5551 CTTAAG
BpuAI 2281 GTCTTC GAAGAC(2/6)
3447 GTCTTC
BsiEI 120 CGACCG CG[GA][CT]^CG
1216 CGGCCG
BsmBI 2847 CGTCTC CGTCTC(1/5)
4063 CGTCTC
Bsu36I 4206 CCTTAGG CC^T[ACGT]AGG
4477 CCTGAGG
DraI 5148 TTTAAA TTT^AAA
5368 TTTAAA
DraIII 3063 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
5296 CACACTGTG
DrdI 1174 GACAACGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4039 GACTACGGGGTC
Eco81I 4206 CCTTAGG CC^T[ACGT]AGG
4477 CCTGAGG
EcoRI 1586 GAATTC G^AATTC
4139 GAATTC
HaeII 1666 AGCGCC [GA]GCGC^[CT]
2052 GGCGCT
HincII 61 GTTAAC GT[CT]^[GA]AC
1837 GTCGAC
HindII 61 GTTAAC GT[CT]^[GA]AC
1837 GTCGAC
NaeI 1287 GCCGGC GCC^GGC
1655 GCCGGC
NdeI 592 CATATG CA^TATG
5407 CATATG
NgoMIV 1287 GCCGGC G^CCGGC
1655 GCCGGC
SacI 4197 GAGCTC GAGCT^C
4875 GAGCTC
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI Alw44I AlwI AlwNI
ApaI ApaLI ApoI Asp700I AspHI
AvaII BalI BanI BanII BbvI
BclI BcnI BfaI BglI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspMI BsrDI
BsrFI BsrGI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoRV
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PshAI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI