##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII Acc65I AccII AclI AgeI
AhdI Aor51HI AscI Asp718I AspEI
BcgI BclI BglII BsaBI BsiWI
BsmBI Bsp1407I BspHI BsrGI BssHII
Bst1107I BstBI BstUI BstZ17I CpoI
Eam1105I Eco47III EcoRI EcoRV FbaI
HgaI HpaI KpnI KspI MamI
MfeI MluI MunI MvnI NotI
NruI NspV PacI PinAI PmeI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SwaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 4495 GTCTAC GT^[AC][GT]AC
AccIII 268 TCCGGA T^CCGGA
AflII 99 CTTAAG C^TTAAG
Alw44I 1296 GTGCAC G^TGCAC
ApaLI 1296 GTGCAC G^TGCAC
AseI 5297 ATTAAT AT^TAAT
AsnI 5297 ATTAAT AT^TAAT
Asp700I 4009 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 3184 TGCGCA TGC^GCA
AvrII 3941 CCTAGG C^CTAGG
BamHI 1128 GGATCC G^GATCC
BbrPI 4320 CACGTG CAC^GTG
BfrI 99 CTTAAG C^TTAAG
BlnI 3941 CCTAGG C^CTAGG
BlpI 3729 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3729 GCTCAGC GC^T[ACGT]AGC
BsaAI 4320 CACGTG [CT]AC^GT[GA]
BsaWI 268 TCCGGA [AT]^CCGG[AT]
BseAI 268 TCCGGA T^CCGGA
BsiEI 5648 CGGCCG CG[GA][CT]^CG
BspDI 4227 ATCGAT AT^CGAT
BspEI 268 TCCGGA T^CCGGA
BspLU11I 565 ACATGT A^CATGT
BstEII 5255 GGTTACC G^GT[ACGT]ACC
BstPI 5255 GGTTACC G^GT[ACGT]ACC
CelII 3729 GCTCAGC GC^T[ACGT]AGC
ClaI 4227 ATCGAT AT^CGAT
EagI 5648 CGGCCG C^GGCCG
EclXI 5648 CGGCCG C^GGCCG
Eco52I 5648 CGGCCG C^GGCCG
EcoO65I 5255 GGTTACC G^GT[ACGT]ACC
FseI 711 GGCCGGCC GGCCGG^CC
FspI 3184 TGCGCA TGC^GCA
MroI 268 TCCGGA T^CCGGA
NaeI 712 GCCGGC GCC^GGC
NdeI 5287 CATATG CA^TATG
NgoMIV 712 GCCGGC G^CCGGC
NheI 1196 GCTAGC G^CTAGC
PmaCI 4320 CACGTG CAC^GTG
PmlI 4320 CACGTG CAC^GTG
PshAI 5206 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5297 ATTAAT AT^TAAT
SspI 3873 AATATT AAT^ATT
XmnI 4009 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 433 ACGTGT A^C[GA][CT]GT
565 ACATGT
BbsI 2963 GAAGAC GAAGAC(2/6)
2015 GTCTTC
BciVI 807 GGATAC GTATCC(6/5)
5585 GGATAC
BglI 1078 GCCAACAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4972 GCCTTGAGGGC
BpuAI 2963 GAAGAC GAAGAC(2/6)
2015 GTCTTC
BsrBI 126 CCGCTC CCGCTC(-3/-3)
1754 CCGCTC
BsrDI 465 CATTGC GCAATG(2/0)
5245 CATTGC
BssSI 5710 CACGAG CACGAG(-5/-1)
5537 CTCGTG
DraI 5111 TTTAAA TTT^AAA
5370 TTTAAA
DraIII 2123 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
3634 CACAAAGTG
DrdI 2966 GACCAGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4158 GACAAAGTGGTC
EcoT22I 375 ATGCAT ATGCA^T
2816 ATGCAT
HincII 3957 GTTGAC GT[CT]^[GA]AC
4167 GTCAAC
HindII 3957 GTTGAC GT[CT]^[GA]AC
4167 GTCAAC
HindIII 4712 AAGCTT A^AGCTT
5015 AAGCTT
NcoI 2248 CCATGG C^CATGG
2500 CCATGG
NsiI 375 ATGCAT ATGCA^T
2816 ATGCAT
PaeR7I 2862 CTCGAG C^TCGAG
3620 CTCGAG
ScaI 4787 AGTACT AGT^ACT
4856 AGTACT
SexAI 2124 ACCTGGT A^CC[AT]GGT
5105 ACCAGGT
XbaI 2621 TCTAGA T^CTAGA
5322 TCTAGA
XhoI 2862 CTCGAG C^TCGAG
3620 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AspI AvaI AvaII BalI
BanI BanII BbeI BbvCI BbvI
BcnI BfaI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NarI NciI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmaCI
XmaI