##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AccII AccIII AgeI
Aor51HI AscI Asp718I BamHI BbeI
BcgI BsaBI BseAI BsiEI BsiWI
BspDI BspEI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
ClaI CpoI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV FseI
KasI KpnI KspI MamI MluI
MroI MvnI NarI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshAI PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI Sse8387I
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AcyI 561 GGCGTC G[GA]^CG[CT]C
AocI 229 CCTGAGG CC^T[ACGT]AGG
AseI 4430 ATTAAT AT^TAAT
AsnI 4430 ATTAAT AT^TAAT
Asp700I 2877 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 3884 TGCGCA TGC^GCA
AvrII 2947 CCTAGG C^CTAGG
BalI 1727 TGGCCA TGG^CCA
BbsI 2061 GTCTTC GAAGAC(2/6)
BbvCI 5473 GCTGAGG CCTCAGC(-5/-2)
BciVI 6022 GGATAC GTATCC(6/5)
BclI 3274 TGATCA T^GATCA
BlnI 2947 CCTAGG C^CTAGG
BlpI 736 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 736 GCTAAGC GC^T[ACGT]AGC
BpuAI 2061 GTCTTC GAAGAC(2/6)
BsaHI 561 GGCGTC G[GA]^CG[CT]C
BsaWI 5568 TCCGGT [AT]^CCGG[AT]
BsmI 1383 GCATTC GAATGC(1/-1)
Bsp1407I 2469 TGTACA T^GTACA
BspLU11I 2908 ACATGT A^CATGT
BspMI 3398 GCAGGT ACCTGC(4/8)
BsrGI 2469 TGTACA T^GTACA
BssSI 2579 CTCGTG CACGAG(-5/-1)
Bsu36I 229 CCTGAGG CC^T[ACGT]AGG
CelII 736 GCTAAGC GC^T[ACGT]AGC
DraIII 487 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 5679 GACTGAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 229 CCTGAGG CC^T[ACGT]AGG
EcoRI 3789 GAATTC G^AATTC
FbaI 3274 TGATCA T^GATCA
FspI 3884 TGCGCA TGC^GCA
Hin1I 561 GGCGTC G[GA]^CG[CT]C
MluNI 1727 TGGCCA TGG^CCA
MscI 1727 TGGCCA TGG^CCA
NaeI 497 GCCGGC GCC^GGC
NgoMIV 497 GCCGGC G^CCGGC
PflMI 2789 CCAGTAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 4430 ATTAAT AT^TAAT
SmaI 504 CCCGGG CCC^GGG
SspBI 2469 TGTACA T^GTACA
Van91I 2789 CCAGTAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 504 CCCGGG C^CCGGG
XmaI 504 CCCGGG C^CCGGG
XmnI 2877 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 1117 GTAGAC GT^[AC][GT]AC
2436 GTAGAC
AclI 4536 AACGTT AA^CGTT
5409 AACGTT
AflII 3365 CTTAAG C^TTAAG
5149 CTTAAG
AflIII 2908 ACATGT A^C[GA][CT]GT
6099 ACACGT
AhdI 1438 GACTCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1777 GACAGCAAGTC
Alw44I 2912 GTGCAC G^TGCAC
3709 GTGCAC
ApaI 941 GGGCCC GGGCC^C
1527 GGGCCC
ApaLI 2912 GTGCAC G^TGCAC
3709 GTGCAC
AspEI 1438 GACTCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1777 GACAGCAAGTC
AspI 838 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2826 GACTGTGTC
BfrI 3365 CTTAAG C^TTAAG
5149 CTTAAG
BglII 670 AGATCT A^GATCT
4333 AGATCT
BspHI 4675 TCATGA T^CATGA
5814 TCATGA
BsrFI 497 GCCGGC [GA]^CCGG[CT]
3893 GCCGGT
BstXI 181 CCATCGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
683 CCAGCCCCTTGG
Cfr10I 497 GCCGGC [GA]^CCGG[CT]
3893 GCCGGT
Eam1105I 1438 GACTCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1777 GACAGCAAGTC
EcoT22I 3773 ATGCAT ATGCA^T
3777 ATGCAT
HaeII 1630 AGCGCC [GA]GCGC^[CT]
1841 GGCGCT
HpaI 3055 GTTAAC GTT^AAC
5734 GTTAAC
MfeI 2342 CAATTG C^AATTG
2465 CAATTG
MunI 2342 CAATTG C^AATTG
2465 CAATTG
NdeI 3287 CATATG CA^TATG
3770 CATATG
NsiI 3773 ATGCAT ATGCA^T
3777 ATGCAT
PflFI 838 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2826 GACTGTGTC
Psp1406I 4536 AACGTT AA^CGTT
5409 AACGTT
RcaI 4675 TCATGA T^CATGA
5814 TCATGA
SapI 1560 GAAGAGC GCTCTTC(1/4)
5882 GAAGAGC
SpeI 4440 ACTAGT A^CTAGT
6037 ACTAGT
StuI 1520 AGGCCT AGG^CCT
4980 AGGCCT
Tth111I 838 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2826 GACTGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BanI BanII BbrPI BbvI BcnI
BfaI BglI BmyI BpmI BsaAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BsrBI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XcmI XhoII