##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII Acc65I AclI AhdI Aor51HI
AscI Asp718I AspEI AspI BbrPI
BciVI BclI BsaAI BsaBI BsiEI
BsiWI Bsp1407I BspDI BspLU11I BsrGI
BssHII BssSI ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I EcoT22I
FbaI FseI HpaI KpnI MamI
MluI NdeI NheI NotI NruI
NsiI PacI PflFI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SalI ScaI SexAI SfiI SnaBI
SpeI SphI SspBI Tth111I
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccI 6281 GTATAC GT^[AC][GT]AC
AflII 193 CTTAAG C^TTAAG
AgeI 1640 ACCGGT A^CCGGT
AseI 5014 ATTAAT AT^TAAT
AsnI 5014 ATTAAT AT^TAAT
AviII 381 TGCGCA TGC^GCA
AvrII 243 CCTAGG C^CTAGG
BfrI 193 CTTAAG C^TTAAG
BlnI 243 CCTAGG C^CTAGG
Bst1107I 6281 GTATAC GTA^TAC
BstBI 1900 TTCGAA TT^CGAA
BstEII 3338 GGTCACC G^GT[ACGT]ACC
BstPI 3338 GGTCACC G^GT[ACGT]ACC
BstZ17I 6281 GTATAC GTA^TAC
DrdI 3947 GACCCAAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3338 GGTCACC G^GT[ACGT]ACC
EcoRI 3478 GAATTC G^AATTC
EcoRV 5425 GATATC GAT^ATC
FspI 381 TGCGCA TGC^GCA
HincII 2911 GTCAAC GT[CT]^[GA]AC
HindII 2911 GTCAAC GT[CT]^[GA]AC
HindIII 5360 AAGCTT A^AGCTT
MfeI 5710 CAATTG C^AATTG
MunI 5710 CAATTG C^AATTG
NcoI 1368 CCATGG C^CATGG
NspV 1900 TTCGAA TT^CGAA
PinAI 1640 ACCGGT A^CCGGT
PshBI 5014 ATTAAT AT^TAAT
SbfI 2407 CCTGCAGG CCTGCA^GG
SfuI 1900 TTCGAA TT^CGAA
SgrAI 1639 CACCGGTG C[GA]^CCGG[CT]G
Sse8387I 2407 CCTGCAGG CCTGCA^GG
SspI 1450 AATATT AAT^ATT
SwaI 2325 ATTTAAAT ATTT^AAAT
XbaI 3394 TCTAGA T^CTAGA
XcmI 3950 CCAAAGGTCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 1852 TCCGGA T^CCGGA
2353 TCCGGA
AcyI 1054 GGCGCC G[GA]^CG[CT]C
2273 GGCGCC
AflIII 4949 ACACGT A^C[GA][CT]GT
5332 ACGTGT
Alw44I 1188 GTGCAC G^TGCAC
5416 GTGCAC
AocI 1066 CCTCAGG CC^T[ACGT]AGG
3541 CCTGAGG
ApaLI 1188 GTGCAC G^TGCAC
5416 GTGCAC
BamHI 1849 GGATCC G^GATCC
2692 GGATCC
BbeI 1054 GGCGCC GGCGC^C
2273 GGCGCC
BglI 112 GCCCCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2780 GCCAGCCTGGC
BsaHI 1054 GGCGCC G[GA]^CG[CT]C
2273 GGCGCC
BseAI 1852 TCCGGA T^CCGGA
2353 TCCGGA
BspEI 1852 TCCGGA T^CCGGA
2353 TCCGGA
BspHI 531 TCATGA T^CATGA
1227 TCATGA
BstAPI 2049 GCACGCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2758 GCAGGGCCTGC
Bsu36I 1066 CCTCAGG CC^T[ACGT]AGG
3541 CCTGAGG
DraI 2326 TTTAAA TTT^AAA
5823 TTTAAA
Eco81I 1066 CCTCAGG CC^T[ACGT]AGG
3541 CCTGAGG
Hin1I 1054 GGCGCC G[GA]^CG[CT]C
2273 GGCGCC
KasI 1054 GGCGCC G^GCGCC
2273 GGCGCC
MroI 1852 TCCGGA T^CCGGA
2353 TCCGGA
MslI 2324 CATTTAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3958 CACATGGATG
NaeI 281 GCCGGC GCC^GGC
356 GCCGGC
NarI 1054 GGCGCC GG^CGCC
2273 GGCGCC
NgoMIV 281 GCCGGC G^CCGGC
356 GCCGGC
PaeR7I 2588 CTCGAG C^TCGAG
3431 CTCGAG
RcaI 531 TCATGA T^CATGA
1227 TCATGA
SfoI 1054 GGCGCC GGC^GCC
2273 GGCGCC
SmaI 457 CCCGGG CCC^GGG
1745 CCCGGG
XhoI 2588 CTCGAG C^TCGAG
3431 CTCGAG
XmaCI 457 CCCGGG C^CCGGG
1745 CCCGGG
XmaI 457 CCCGGG C^CCGGG
1745 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcgI
BcnI BfaI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI SacII SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI