##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AclI AgeI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
AviII AvrII BcgI BclI BlnI
BsaBI BsiWI BsmI BspDI BspHI
BspLU11I BssHII BssSI BstBI ClaI
CpoI DraI DraIII EagI EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
MamI MfeI MluI MslI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SapI ScaI SfuI
SgrAI SnaBI SpeI SspI SwaI
TfiI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 2465 GGTACC G^GTACC
AccI 4417 GTATAC GT^[AC][GT]AC
AcsI 2380 AAATTC [GA]^AATT[CT]
AflII 1216 CTTAAG C^TTAAG
AhdI 3004 GACTCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 2380 AAATTC [GA]^AATT[CT]
Asp718I 2465 GGTACC G^GTACC
AspEI 3004 GACTCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 2786 CACGTG CAC^GTG
BfaI 3890 CTAG C^TAG
BfrI 1216 CTTAAG C^TTAAG
BglII 4066 AGATCT A^GATCT
BsiEI 52 CGACCG CG[GA][CT]^CG
Bst1107I 4417 GTATAC GTA^TAC
BstEII 3650 GGTGACC G^GT[ACGT]ACC
BstPI 3650 GGTGACC G^GT[ACGT]ACC
BstXI 2344 CCACCCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4417 GTATAC GTA^TAC
DrdI 2275 GACGAGTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3004 GACTCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3650 GGTGACC G^GT[ACGT]ACC
HincII 607 GTCAAC GT[CT]^[GA]AC
HindII 607 GTCAAC GT[CT]^[GA]AC
KpnI 2465 GGTACC GGTAC^C
MaeI 3890 CTAG C^TAG
NspI 3566 GCATGC [GA]CATG^[CT]
PmaCI 2786 CACGTG CAC^GTG
PmlI 2786 CACGTG CAC^GTG
SexAI 911 ACCTGGT A^CC[AT]GGT
SfiI 1040 GGCCACCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1142 CCCGGG CCC^GGG
SphI 3566 GCATGC GCATG^C
StuI 1503 AGGCCT AGG^CCT
XcmI 2426 CCAGTAATTATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1142 CCCGGG C^CCGGG
XmaI 1142 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 2785 ACACGT A^C[GA][CT]GT
3275 ACACGT
AspI 910 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1699 GACTCTGTC
BalI 2186 TGGCCA TGG^CCA
3512 TGGCCA
BbsI 1696 GAAGAC GAAGAC(2/6)
2149 GAAGAC
BciVI 228 GGATAC GTATCC(6/5)
3439 GGATAC
BpuAI 1696 GAAGAC GAAGAC(2/6)
2149 GAAGAC
BsgI 2612 CTGCAC GTGCAG(16/14)
3271 CTGCAC
BsrDI 2801 CATTGC GCAATG(2/0)
1874 GCAATG
BsrFI 693 GCCGGC [GA]^CCGG[CT]
2236 GCCGGC
BstAPI 183 GCAGGAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3561 GCAGTGCATGC
Cfr10I 693 GCCGGC [GA]^CCGG[CT]
2236 GCCGGC
EcoNI 3537 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4167 CCTGGGGCAGG
MluNI 2186 TGGCCA TGG^CCA
3512 TGGCCA
MscI 2186 TGGCCA TGG^CCA
3512 TGGCCA
NaeI 693 GCCGGC GCC^GGC
2236 GCCGGC
NcoI 111 CCATGG C^CATGG
389 CCATGG
NgoMIV 693 GCCGGC G^CCGGC
2236 GCCGGC
PflFI 910 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1699 GACTCTGTC
Tth111I 910 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1699 GACTCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BamHI BanI
BanII BbeI BbvCI BbvI BcnI
BglI BlpI BmyI BpmI Bpu1102I
BsaAI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
Bsp1407I BspEI BspMI BsrBI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeII MaeIII
MboI MboII MflI MnlI MroI
MseI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV PaeR7I PflMI PleI PpuMI
PstI PvuII RsaI SacII Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SfoI SmlI Sse8387I SspBI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoI XhoII