##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
Acc65I AgeI AhdI Aor51HI AscI
Asp718I AspEI AviII AvrII BcgI
BlnI BsaBI BsiWI BspDI BspLU11I
BssHII ClaI CpoI Eam1105I Eco47III
EcoRV FseI FspI HpaI KpnI
KspI MamI MluI NheI NotI
NruI PacI PaeR7I PinAI PmeI
PvuI RsrII SacII SalI SbfI
SexAI SgrAI SnaBI SpeI Sse8387I
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 2642 GACGTC GACGT^C
AccIII 5772 TCCGGA T^CCGGA
AflIII 2111 ACACGT A^C[GA][CT]GT
Alw44I 4173 GTGCAC G^TGCAC
ApaLI 4173 GTGCAC G^TGCAC
BbvCI 1099 GCTGAGG CCTCAGC(-5/-2)
BglII 5506 AGATCT A^GATCT
BlpI 526 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 526 GCTCAGC GC^T[ACGT]AGC
BseAI 5772 TCCGGA T^CCGGA
BsiEI 2843 CGGCCG CG[GA][CT]^CG
BsmBI 996 GAGACG CGTCTC(1/5)
BspEI 5772 TCCGGA T^CCGGA
Bst1107I 167 GTATAC GTA^TAC
BstAPI 6361 GCAACGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 5630 TTCGAA TT^CGAA
BstZ17I 167 GTATAC GTA^TAC
CelII 526 GCTCAGC GC^T[ACGT]AGC
DraIII 3254 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 3122 CCATGG C^C[GA][CT]GG
EagI 2843 CGGCCG C^GGCCG
EclXI 2843 CGGCCG C^GGCCG
Eco52I 2843 CGGCCG C^GGCCG
MfeI 221 CAATTG C^AATTG
MroI 5772 TCCGGA T^CCGGA
MunI 221 CAATTG C^AATTG
NcoI 3122 CCATGG C^CATGG
NdeI 3485 CATATG CA^TATG
NspV 5630 TTCGAA TT^CGAA
PshAI 5713 GACTTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 3797 CTGCAG CTGCA^G
SacI 4690 GAGCTC GAGCT^C
ScaI 4973 AGTACT AGT^ACT
SfiI 494 GGCCCAAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 5630 TTCGAA TT^CGAA
SphI 2312 GCATGC GCATG^C
XbaI 6492 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
AccII 604 CGCG CG^CG
5878 CGCG
AclI 1491 AACGTT AA^CGTT
6363 AACGTT
AlwNI 3219 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
5203 CAGGGGCTG
AseI 237 ATTAAT AT^TAAT
2280 ATTAAT
AsnI 237 ATTAAT AT^TAAT
2280 ATTAAT
BamHI 60 GGATCC G^GATCC
1185 GGATCC
BbrPI 3552 CACGTG CAC^GTG
5221 CACGTG
BciVI 2030 GGATAC GTATCC(6/5)
2457 GTATCC
BglI 495 GCCCAAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3289 GCCCTTGGGGC
BsaAI 3552 CACGTG [CT]AC^GT[GA]
5221 CACGTG
BsgI 5146 CTGCAC GTGCAG(16/14)
3260 GTGCAG
BsmI 2409 GAATGC GAATGC(1/-1)
4654 GAATGC
BspHI 884 TCATGA T^CATGA
4073 TCATGA
BsrFI 563 GCCGGC [GA]^CCGG[CT]
2841 GCCGGC
BssSI 2103 CACGAG CACGAG(-5/-1)
1180 CTCGTG
BstEII 277 GGTGACC G^GT[ACGT]ACC
1071 GGTGACC
BstPI 277 GGTGACC G^GT[ACGT]ACC
1071 GGTGACC
BstUI 604 CGCG CG^CG
5878 CGCG
Cfr10I 563 GCCGGC [GA]^CCGG[CT]
2841 GCCGGC
DrdI 472 GACAATGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5440 GACATCTACGTC
EcoNI 2701 CCTCCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4210 CCTTATGCAGG
EcoO65I 277 GGTGACC G^GT[ACGT]ACC
1071 GGTGACC
EcoRI 2369 GAATTC G^AATTC
6524 GAATTC
EcoT22I 2411 ATGCAT ATGCA^T
2933 ATGCAT
HincII 745 GTCAAC GT[CT]^[GA]AC
2898 GTTGAC
HindII 745 GTCAAC GT[CT]^[GA]AC
2898 GTTGAC
HindIII 1657 AAGCTT A^AGCTT
2751 AAGCTT
MvnI 604 CGCG CG^CG
5878 CGCG
NaeI 563 GCCGGC GCC^GGC
2841 GCCGGC
NgoMIV 563 GCCGGC G^CCGGC
2841 GCCGGC
NsiI 2411 ATGCAT ATGCA^T
2933 ATGCAT
PmaCI 3552 CACGTG CAC^GTG
5221 CACGTG
PmlI 3552 CACGTG CAC^GTG
5221 CACGTG
PpuMI 266 AGGACCT [GA]G^G[AT]CC[CT]
4948 GGGTCCC
PshBI 237 ATTAAT AT^TAAT
2280 ATTAAT
Psp1406I 1491 AACGTT AA^CGTT
6363 AACGTT
RcaI 884 TCATGA T^CATGA
4073 TCATGA
SmaI 2824 CCCGGG CCC^GGG
4886 CCCGGG
SspI 1149 AATATT AAT^ATT
6433 AATATT
StuI 649 AGGCCT AGG^CCT
3892 AGGCCT
XmaCI 2824 CCCGGG C^CCGGG
4886 CCCGGG
XmaI 2824 CCCGGG C^CCGGG
4886 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflII AluI AlwI AocI ApaI
ApoI Asp700I AspHI AspI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvI BclI BcnI BfaI
BfrI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrBI BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NarI NciI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmnI