##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
Acc65I AccI AccIII AflIII Aor51HI
ApaI AscI Asp718I AviII BamHI
BbeI BclI BglI BseAI BsiEI
BsiWI BspDI BspEI BspLU11I BsrBI
BssHII BssSI Bst1107I BstEII BstPI
BstZ17I ClaI CpoI DraII DrdI
EagI EclXI Eco47III Eco52I EcoO109I
EcoO65I FauI FbaI FseI FspI
HindIII KasI KpnI KspI MluI
MroI NaeI NarI NgoMIV NheI
NotI NruI PacI PmeI PpuMI
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfoI SgrAI SnaBI
Sse8387I StuI XbaI XcmI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AatII 2020 GACGTC GACGT^C
AccII 1932 CGCG CG^CG
AclI 2468 AACGTT AA^CGTT
AflII 2786 CTTAAG C^TTAAG
AgeI 2335 ACCGGT A^CCGGT
AhdI 2015 GACAAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4254 GTGCAC G^TGCAC
ApaLI 4254 GTGCAC G^TGCAC
AseI 3423 ATTAAT AT^TAAT
AsnI 3423 ATTAAT AT^TAAT
AspEI 2015 GACAAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3988 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2513 CCTAGG C^CTAGG
BanI 815 GGTGCC G^G[CT][GA]CC
BbrPI 3537 CACGTG CAC^GTG
BbvCI 2880 CCTCAGC CCTCAGC(-5/-2)
BcgI 91 CGAGAGGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2786 CTTAAG C^TTAAG
BlnI 2513 CCTAGG C^CTAGG
BsaAI 3537 CACGTG [CT]AC^GT[GA]
BsaI 1514 GAGACC GGTCTC(1/5)
BseRI 804 GAGGAG GAGGAG(10/8)
BsmBI 4149 GAGACG CGTCTC(1/5)
BsrFI 2335 ACCGGT [GA]^CCGG[CT]
BstBI 204 TTCGAA TT^CGAA
BstUI 1932 CGCG CG^CG
Cfr10I 2335 ACCGGT [GA]^CCGG[CT]
DraIII 2030 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2015 GACAAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1075 CCTTTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2273 GATATC GAT^ATC
EcoT22I 2666 ATGCAT ATGCA^T
HaeII 2 AGCGCC [GA]GCGC^[CT]
MfeI 1820 CAATTG C^AATTG
MunI 1820 CAATTG C^AATTG
MvnI 1932 CGCG CG^CG
NdeI 2663 CATATG CA^TATG
NsiI 2666 ATGCAT ATGCA^T
NspV 204 TTCGAA TT^CGAA
PaeR7I 171 CTCGAG C^TCGAG
PflFI 3988 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 858 CCAACAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2335 ACCGGT A^CCGGT
PmaCI 3537 CACGTG CAC^GTG
PmlI 3537 CACGTG CAC^GTG
PshAI 1484 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3423 ATTAAT AT^TAAT
Psp1406I 2468 AACGTT AA^CGTT
SacI 3469 GAGCTC GAGCT^C
ScaI 3570 AGTACT AGT^ACT
SfuI 204 TTCGAA TT^CGAA
SmaI 4058 CCCGGG CCC^GGG
SpeI 3304 ACTAGT A^CTAGT
SwaI 3367 ATTTAAAT ATTT^AAAT
Tth111I 3988 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 858 CCAACAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 171 CTCGAG C^TCGAG
XmaCI 4058 CCCGGG C^CCGGG
XmaI 4058 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AlwNI 2099 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
2370 CAGTAACTG
AocI 3922 CCTCAGG CC^T[ACGT]AGG
4196 CCTCAGG
Asp700I 115 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2854 GAAGATCTTC
AspHI 3469 GAGCTC G[AT]GC[AT]^C
4254 GTGCAC
AvaI 171 CTCGAG C^[CT]CG[GA]G
4058 CCCGGG
BalI 329 TGGCCA TGG^CCA
2485 TGGCCA
BanII 1146 GAGCCC G[GA]GC[CT]^C
3469 GAGCTC
BbsI 29 GAAGAC GAAGAC(2/6)
1813 GAAGAC
BciVI 1262 GGATAC GTATCC(6/5)
2814 GGATAC
BcnI 4058 CCCGG CC^[GC]GG
4059 CCGGG
BglII 1786 AGATCT A^GATCT
2856 AGATCT
BlpI 2768 GCTGAGC GC^T[ACGT]AGC
2940 GCTTAGC
BpmI 3220 CTCCAG CTGGAG(16/14)
4014 CTGGAG
Bpu1102I 2768 GCTGAGC GC^T[ACGT]AGC
2940 GCTTAGC
BpuAI 29 GAAGAC GAAGAC(2/6)
1813 GAAGAC
BsaWI 1968 ACCGGA [AT]^CCGG[AT]
2335 ACCGGT
BsiHKAI 3469 GAGCTC G[AT]GC[AT]^C
4254 GTGCAC
BsoBI 171 CTCGAG C^[CT]CG[GA]G
4058 CCCGGG
BstXI 410 CCAGGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4161 CCACCATATTGG
BstYI 1786 AGATCT [GA]^GATC[CT]
2856 AGATCT
Bsu36I 3922 CCTCAGG CC^T[ACGT]AGG
4196 CCTCAGG
CelII 2768 GCTGAGC GC^T[ACGT]AGC
2940 GCTTAGC
DsaI 104 CCATGG C^C[GA][CT]GG
1760 CCATGG
EaeI 329 TGGCCA [CT]^GGCC[GA]
2485 TGGCCA
Eco81I 3922 CCTCAGG CC^T[ACGT]AGG
4196 CCTCAGG
HgaI 1199 GACGC GACGC(5/10)
4300 GCGTC
HpaI 1130 GTTAAC GTT^AAC
3300 GTTAAC
MflI 1786 AGATCT [GA]^GATC[CT]
2856 AGATCT
MluNI 329 TGGCCA TGG^CCA
2485 TGGCCA
MscI 329 TGGCCA TGG^CCA
2485 TGGCCA
NciI 4058 CCCGG CC^[GC]GG
4059 CCGGG
NcoI 104 CCATGG C^CATGG
1760 CCATGG
SapI 3466 GAAGAGC GCTCTTC(1/4)
4340 GAAGAGC
XhoII 1786 AGATCT [GA]^GATC[CT]
2856 AGATCT
XmnI 115 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2854 GAAGATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AvaII BbvI BfaI
BmyI BsaBI BsaHI BsaJI BsgI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspHI BspMI BsrDI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraI EarI EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I