##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AccIII AgeI Alw44I ApaLI
AscI AvaI AviII AvrII BamHI
BbrPI BciVI BlnI BseAI BsiEI
BsiWI BsoBI BspEI BssHII BstBI
BstEII BstPI DraIII EagI EclXI
Eco52I EcoO65I EcoRI FseI FspI
HindIII KspI MluI MroI NheI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PvuI SacII SalI SbfI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SwaI Van91I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 4938 GGTACC G^GTACC
AclI 3391 AACGTT AA^CGTT
AcyI 5303 GGCGCC G[GA]^CG[CT]C
ApaI 2267 GGGCCC GGGCC^C
AseI 4672 ATTAAT AT^TAAT
AsnI 4672 ATTAAT AT^TAAT
Asp718I 4938 GGTACC G^GTACC
BbeI 5303 GGCGCC GGCGC^C
BbvCI 5274 CCTCAGC CCTCAGC(-5/-2)
BcgI 5263 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1789 GCCAGACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 5686 CACGTA [CT]AC^GT[GA]
BsaBI 854 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 5303 GGCGCC G[GA]^CG[CT]C
BsaWI 87 TCCGGT [AT]^CCGG[AT]
Bsp1407I 4731 TGTACA T^GTACA
BspDI 4224 ATCGAT AT^CGAT
BspHI 5393 TCATGA T^CATGA
BspLU11I 3530 ACATGT A^CATGT
BspMI 5992 ACCTGC ACCTGC(4/8)
BsrDI 3144 CATTGC GCAATG(2/0)
BsrGI 4731 TGTACA T^GTACA
BssSI 74 CACGAG CACGAG(-5/-1)
ClaI 4224 ATCGAT AT^CGAT
CpoI 84 CGGTCCG CG^G[AT]CCG
DrdI 5651 GACTTTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2416 CCATGG C^C[GA][CT]GG
EcoRV 2508 GATATC GAT^ATC
EcoT22I 6019 ATGCAT ATGCA^T
Hin1I 5303 GGCGCC G[GA]^CG[CT]C
HpaI 3185 GTTAAC GTT^AAC
KasI 5303 GGCGCC G^GCGCC
KpnI 4938 GGTACC GGTAC^C
MamI 854 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2197 GCCGGC GCC^GGC
NarI 5303 GGCGCC GG^CGCC
NcoI 2416 CCATGG C^CATGG
NdeI 950 CATATG CA^TATG
NgoMIV 2197 GCCGGC G^CCGGC
NsiI 6019 ATGCAT ATGCA^T
PshBI 4672 ATTAAT AT^TAAT
Psp1406I 3391 AACGTT AA^CGTT
RcaI 5393 TCATGA T^CATGA
RsrII 84 CGGTCCG CG^G[AT]CCG
SacI 765 GAGCTC GAGCT^C
ScaI 2537 AGTACT AGT^ACT
SfoI 5303 GGCGCC GGC^GCC
SspBI 4731 TGTACA T^GTACA
StuI 3998 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflII 3339 CTTAAG C^TTAAG
3654 CTTAAG
AflIII 252 ACACGT A^C[GA][CT]GT
3530 ACATGT
AhdI 2495 GACTTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4502 GACCATTTGTC
Aor51HI 2699 AGCGCT AGC^GCT
4286 AGCGCT
AspEI 2495 GACTTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4502 GACCATTTGTC
AspI 1157 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
5189 GACTGAGTC
BfrI 3339 CTTAAG C^TTAAG
3654 CTTAAG
BsmBI 699 CGTCTC CGTCTC(1/5)
1751 CGTCTC
Bst1107I 3421 GTATAC GTA^TAC
4531 GTATAC
BstXI 2416 CCATGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5417 CCAGAGTGCTGG
BstZ17I 3421 GTATAC GTA^TAC
4531 GTATAC
Eam1105I 2495 GACTTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4502 GACCATTTGTC
Eco47III 2699 AGCGCT AGC^GCT
4286 AGCGCT
HgaI 202 GACGC GACGC(5/10)
333 GCGTC
HincII 1138 GTTGAC GT[CT]^[GA]AC
3185 GTTAAC
HindII 1138 GTTGAC GT[CT]^[GA]AC
3185 GTTAAC
MfeI 2549 CAATTG C^AATTG
5845 CAATTG
MunI 2549 CAATTG C^AATTG
5845 CAATTG
PflFI 1157 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
5189 GACTGAGTC
PpuMI 2723 AGGTCCC [GA]G^G[AT]CC[CT]
3951 GGGACCC
PshAI 3851 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5522 GACTCTTGTC
SpeI 1055 ACTAGT A^CTAGT
5941 ACTAGT
SspI 3284 AATATT AAT^ATT
4836 AATATT
Tth111I 1157 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
5189 GACTGAGTC
XbaI 659 TCTAGA T^CTAGA
6024 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AvaII BalI BanI
BanII BbsI BbvI BclI BcnI
BfaI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BsrBI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI