##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaLI AscI
Asp718I AspEI AviII BbrPI BciVI
BclI BglI BlpI Bpu1102I BsaWI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspHI BsrGI BssHII Bst1107I
BstBI BstZ17I CelII ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRV EcoT22I FbaI FseI
FspI KpnI KspI MluI MroI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RcaI RsrII
SacII SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccI 4388 GTCGAC GT^[AC][GT]AC
AflII 1850 CTTAAG C^TTAAG
AflIII 1212 ACATGT A^C[GA][CT]GT
AvaII 715 GGACC G^G[AT]CC
AvrII 2410 CCTAGG C^CTAGG
BbeI 3535 GGCGCC GGCGC^C
BfrI 1850 CTTAAG C^TTAAG
BglII 2572 AGATCT A^GATCT
BlnI 2410 CCTAGG C^CTAGG
BsaAI 3908 CACGTA [CT]AC^GT[GA]
BsaBI 4649 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 3582 GAGACG CGTCTC(1/5)
BspLU11I 1212 ACATGT A^CATGT
BsrFI 2944 GCCGGT [GA]^CCGG[CT]
BssSI 3629 CTCGTG CACGAG(-5/-1)
BstAPI 937 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1486 GGTGACC G^GT[ACGT]ACC
BstPI 1486 GGTGACC G^GT[ACGT]ACC
BstXI 1005 CCAGTTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 2944 GCCGGT [GA]^CCGG[CT]
DraIII 5491 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1486 GGTGACC G^GT[ACGT]ACC
EcoRI 2185 GAATTC G^AATTC
HaeII 3535 GGCGCC [GA]GCGC^[CT]
KasI 3535 GGCGCC G^GCGCC
MamI 4649 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 3535 GGCGCC GG^CGCC
SalI 4388 GTCGAC G^TCGAC
SapI 917 GAAGAGC GCTCTTC(1/4)
SfoI 3535 GGCGCC GGC^GCC
SmaI 3483 CCCGGG CCC^GGG
SphI 2605 GCATGC GCATG^C
SspI 572 AATATT AAT^ATT
XmaCI 3483 CCCGGG C^CCGGG
XmaI 3483 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 3453 CGCG CG^CG
3684 CGCG
AcyI 1788 GGCGTC G[GA]^CG[CT]C
3535 GGCGCC
AseI 3733 ATTAAT AT^TAAT
5083 ATTAAT
AsnI 3733 ATTAAT AT^TAAT
5083 ATTAAT
AspI 1795 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3413 GACAGAGTC
AvaI 2731 CTCGGG C^[CT]CG[GA]G
3483 CCCGGG
BamHI 98 GGATCC G^GATCC
4196 GGATCC
BcgI 1636 CGAGCGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
87 GCATAGATTTCG
BsaHI 1788 GGCGTC G[GA]^CG[CT]C
3535 GGCGCC
BsoBI 2731 CTCGGG C^[CT]CG[GA]G
3483 CCCGGG
BsrBI 3461 CCGCTC CCGCTC(-3/-3)
1637 GAGCGG
BstUI 3453 CGCG CG^CG
3684 CGCG
HgaI 4430 GACGC GACGC(5/10)
1789 GCGTC
Hin1I 1788 GGCGTC G[GA]^CG[CT]C
3535 GGCGCC
HpaI 5801 GTTAAC GTT^AAC
5904 GTTAAC
MfeI 2401 CAATTG C^AATTG
3273 CAATTG
MunI 2401 CAATTG C^AATTG
3273 CAATTG
MvnI 3453 CGCG CG^CG
3684 CGCG
PflFI 1795 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3413 GACAGAGTC
PshBI 3733 ATTAAT AT^TAAT
5083 ATTAAT
PstI 1428 CTGCAG CTGCA^G
2802 CTGCAG
ScaI 467 AGTACT AGT^ACT
1421 AGTACT
Tth111I 1795 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3413 GACAGAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI Asp700I
AspHI BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI