##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AgeI Alw44I AocI
ApaLI AscI AseI AsnI Asp700I
Asp718I AviII BamHI BbeI BbrPI
BcgI BclI BpmI BsaAI BsaBI
BsiWI BsmBI Bsp1407I BspDI BsrGI
BstAPI BstEII BstPI Bsu36I ClaI
CpoI EagI EclXI Eco52I Eco81I
EcoO65I EcoT22I FbaI FseI FspI
HindIII HpaI KasI KpnI MamI
MluI NarI NcoI NheI NotI
NsiI PacI PinAI PmaCI PmeI
PmlI PshAI PshBI RsrII SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI XcmI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 4576 GTATAC GT^[AC][GT]AC
AclI 1982 AACGTT AA^CGTT
AcyI 386 GACGCC G[GA]^CG[CT]C
AflII 3083 CTTAAG C^TTAAG
AhdI 1092 GACCTCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1374 AGCGCT AGC^GCT
AspEI 1092 GACCTCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1163 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2362 TGGCCA TGG^CCA
BbvCI 5 GCTGAGG CCTCAGC(-5/-2)
BciVI 3957 GTATCC GTATCC(6/5)
BfrI 3083 CTTAAG C^TTAAG
BsaHI 386 GACGCC G[GA]^CG[CT]C
BsiEI 134 CGATCG CG[GA][CT]^CG
BspHI 2977 TCATGA T^CATGA
BsrDI 3994 GCAATG GCAATG(2/0)
BssHII 511 GCGCGC G^CGCGC
Bst1107I 4576 GTATAC GTA^TAC
BstBI 3384 TTCGAA TT^CGAA
BstXI 2829 CCATATGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4576 GTATAC GTA^TAC
DraIII 557 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2183 GACTGCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 827 CCGCGG C^C[GA][CT]GG
Eam1105I 1092 GACCTCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1374 AGCGCT AGC^GCT
EcoRI 4729 GAATTC G^AATTC
EcoRV 4502 GATATC GAT^ATC
Hin1I 386 GACGCC G[GA]^CG[CT]C
KspI 827 CCGCGG CCGC^GG
MfeI 4926 CAATTG C^AATTG
MluNI 2362 TGGCCA TGG^CCA
MscI 2362 TGGCCA TGG^CCA
MunI 4926 CAATTG C^AATTG
NdeI 2830 CATATG CA^TATG
NruI 1010 TCGCGA TCG^CGA
NspV 3384 TTCGAA TT^CGAA
PaeR7I 2612 CTCGAG C^TCGAG
PflFI 1163 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 1982 AACGTT AA^CGTT
PvuI 134 CGATCG CGAT^CG
RcaI 2977 TCATGA T^CATGA
SacII 827 CCGCGG CCGC^GG
SapI 17 GAAGAGC GCTCTTC(1/4)
SfuI 3384 TTCGAA TT^CGAA
SspI 5070 AATATT AAT^ATT
Tth111I 1163 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2612 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
ApaI 144 GGGCCC GGGCC^C
3059 GGGCCC
AvrII 2597 CCTAGG C^CTAGG
3241 CCTAGG
BanI 2607 GGTGCC G^G[CT][GA]CC
2950 GGCACC
BglII 2257 AGATCT A^GATCT
5190 AGATCT
BlnI 2597 CCTAGG C^CTAGG
3241 CCTAGG
BsaI 912 GAGACC GGTCTC(1/5)
2174 GAGACC
BsmI 2912 GAATGC GAATGC(1/-1)
3650 GCATTC
BspMI 1829 ACCTGC ACCTGC(4/8)
2133 GCAGGT
EcoNI 624 CCTCCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3079 CCTCCTTAAGG
HaeII 1374 AGCGCT [GA]GCGC^[CT]
2279 AGCGCC
MslI 3706 CATACACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4248 CATACTAATG
NaeI 84 GCCGGC GCC^GGC
1750 GCCGGC
NgoMIV 84 GCCGGC G^CCGGC
1750 GCCGGC
PpuMI 965 GGGACCC [GA]G^G[AT]CC[CT]
2600 AGGTCCT
SacI 1014 GAGCTC GAGCT^C
2615 GAGCTC
TaqI 2613 TCGA T^CGA
3385 TCGA
TthHB8I 2613 TCGA T^CGA
3385 TCGA
XbaI 3159 TCTAGA T^CTAGA
4073 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AccIII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BanII BbsI
BbvI BcnI BfaI BglI BlpI
BmyI Bpu1102I BpuAI BsaJI BsaWI
BseAI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspEI BspLU11I BsrBI BsrFI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MroI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XhoII