##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
Acc65I AccIII AclI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BglI BlnI BseAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BssHII BstBI Bsu36I CfoI
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV FseI FspI HaeII HhaI
HinP1I KasI KpnI MroI NaeI
NarI NgoMIV NotI NruI NspV
PacI PaeR7I PmaCI PmeI PmlI
PpuMI Psp1406I PvuI RsrII SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SpeI Sse8387I StuI SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 979 GACGTC GACGT^C
AcyI 979 GACGTC G[GA]^CG[CT]C
AflII 3291 CTTAAG C^TTAAG
AgeI 3981 ACCGGT A^CCGGT
BclI 346 TGATCA T^GATCA
BcnI 4805 CCGGG CC^[GC]GG
BfrI 3291 CTTAAG C^TTAAG
BlpI 815 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 815 GCTAAGC GC^T[ACGT]AGC
BsaAI 3965 TACGTA [CT]AC^GT[GA]
BsaHI 979 GACGTC G[GA]^CG[CT]C
BsaWI 3981 ACCGGT [AT]^CCGG[AT]
BseRI 1039 CTCCTC GAGGAG(10/8)
BsrBI 1148 CCGCTC CCGCTC(-3/-3)
BsrDI 3870 GCAATG GCAATG(2/0)
BsrFI 3981 ACCGGT [GA]^CCGG[CT]
BssSI 2203 CTCGTG CACGAG(-5/-1)
BstEII 4974 GGTTACC G^GT[ACGT]ACC
BstPI 4974 GGTTACC G^GT[ACGT]ACC
CelII 815 GCTAAGC GC^T[ACGT]AGC
Cfr10I 3981 ACCGGT [GA]^CCGG[CT]
DraII 4807 GGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 1495 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 2112 CCTACACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 4807 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 4974 GGTTACC G^GT[ACGT]ACC
FbaI 346 TGATCA T^GATCA
Hin1I 979 GACGTC G[GA]^CG[CT]C
KspI 10 CCGCGG CCGC^GG
NciI 4805 CCGGG CC^[GC]GG
NdeI 5378 CATATG CA^TATG
NheI 4317 GCTAGC G^CTAGC
PinAI 3981 ACCGGT A^CCGGT
PshAI 1485 GACATAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 2311 CAGCTG CAG^CTG
SacI 4908 GAGCTC GAGCT^C
SacII 10 CCGCGG CCGC^GG
SalI 1792 GTCGAC G^TCGAC
SnaBI 3965 TACGTA TAC^GTA
SphI 5026 GCATGC GCATG^C
Tsp45I 2162 GTCAC ^GT[GC]AC
XbaI 4204 TCTAGA T^CTAGA
XcmI 1267 CCATGAATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 45 enzymes) #
###################################
AlwNI 583 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2311 CAGCTGCTG
Asp700I 369 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1164 GAAGGATTTC
AspI 146 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2059 GACCTGGTC
BalI 1235 TGGCCA TGG^CCA
1987 TGGCCA
BanII 4908 GAGCTC G[GA]GC[CT]^C
5304 GAGCCC
BglII 2679 AGATCT A^GATCT
4928 AGATCT
BsaBI 1104 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3077 GATATTAATC
BsaI 466 GAGACC GGTCTC(1/5)
2057 GAGACC
BsgI 1281 GTGCAG GTGCAG(16/14)
1624 GTGCAG
Bsp1407I 4230 TGTACA T^GTACA
5331 TGTACA
BspLU11I 2431 ACATGT A^CATGT
5334 ACATGT
BspMI 1620 GCAGGT ACCTGC(4/8)
2383 GCAGGT
BsrGI 4230 TGTACA T^GTACA
5331 TGTACA
Bst1107I 4692 GTATAC GTA^TAC
5180 GTATAC
BstAPI 4491 GCAGAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5021 GCAAGGCATGC
BstXI 1444 CCAAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1655 CCACAGCAGTGG
BstZ17I 4692 GTATAC GTA^TAC
5180 GTATAC
EaeI 1235 TGGCCA [CT]^GGCC[GA]
1987 TGGCCA
EcoT22I 3585 ATGCAT ATGCA^T
5375 ATGCAT
FauI 1147 CCCGC CCCGC(4/6)
2103 GCGGG
HapII 3982 CCGG C^CGG
4805 CCGG
HgaI 15 GACGC GACGC(5/10)
6 GCGTC
HindIII 195 AAGCTT A^AGCTT
1869 AAGCTT
HpaII 3982 CCGG C^CGG
4805 CCGG
MamI 1104 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3077 GATATTAATC
MfeI 771 CAATTG C^AATTG
4212 CAATTG
MluI 4 ACGCGT A^CGCGT
16 ACGCGT
MluNI 1235 TGGCCA TGG^CCA
1987 TGGCCA
MscI 1235 TGGCCA TGG^CCA
1987 TGGCCA
MslI 1656 CACAGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1683 CATGACCATG
MspA1I 10 CCGCGG C[AC]G^C[GT]G
2311 CAGCTG
MspI 3982 CCGG C^CGG
4805 CCGG
MunI 771 CAATTG C^AATTG
4212 CAATTG
NcoI 1288 CCATGG C^CATGG
3132 CCATGG
NsiI 3585 ATGCAT ATGCA^T
5375 ATGCAT
PflFI 146 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2059 GACCTGGTC
PflMI 767 CCAACAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1238 CCAGACCTTGG
PleI 1155 GACTC GAGTC(4/5)
1790 GAGTC
SspBI 4230 TGTACA T^GTACA
5331 TGTACA
SspI 3448 AATATT AAT^ATT
4674 AATATT
TaqI 1793 TCGA T^CGA
2069 TCGA
Tth111I 146 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2059 GACCTGGTC
TthHB8I 1793 TCGA T^CGA
2069 TCGA
Van91I 767 CCAACAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1238 CCAGACCTTGG
XmnI 369 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1164 GAAGGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI ApoI AseI
AsnI AspHI AvaII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspHI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
Cfr13I DdeI DpnII DraI DsaI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HincII HindII HinfI HpaI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MvaI MvnI MwoI NdeII
NlaIII NlaIV NspI PshBI PstI
RcaI RsaI Sau3AI Sau96I ScaI
ScrFI SexAI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp509I
TspRI XhoII