##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AccI AccII AclI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI AspEI
AvaI AviII BbrPI BcgI BclI
BcnI BfrI BglI BsiWI BsmI
BsoBI BspDI BspLU11I BsrFI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoO65I EcoT22I FbaI FseI FspI
HpaI KspI MluI MvnI NaeI
NciI NcoI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SbfI SfiI SfuI
SgrAI SmaI SmlI Sse8387I SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 1159 GACGTC GACGT^C
Acc65I 729 GGTACC G^GTACC
AccIII 1107 TCCGGA T^CCGGA
Asp718I 729 GGTACC G^GTACC
AspI 287 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1564 CCTAGG C^CTAGG
BamHI 1022 GGATCC G^GATCC
BbeI 2048 GGCGCC GGCGC^C
BciVI 2788 GTATCC GTATCC(6/5)
BlnI 1564 CCTAGG C^CTAGG
BlpI 372 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 372 GCTCAGC GC^T[ACGT]AGC
BsaAI 4977 TACGTA [CT]AC^GT[GA]
BsaBI 1436 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1107 TCCGGA [AT]^CCGG[AT]
BseAI 1107 TCCGGA T^CCGGA
BsiEI 1251 CGGCCG CG[GA][CT]^CG
BspEI 1107 TCCGGA T^CCGGA
BsrBI 913 CCGCTC CCGCTC(-3/-3)
BssSI 935 CACGAG CACGAG(-5/-1)
BstAPI 4013 GCATCATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 372 GCTCAGC GC^T[ACGT]AGC
DraI 3618 TTTAAA TTT^AAA
DsaI 1038 CCACGG C^C[GA][CT]GG
EagI 1251 CGGCCG C^GGCCG
EclXI 1251 CGGCCG C^GGCCG
Eco52I 1251 CGGCCG C^GGCCG
EcoRI 3891 GAATTC G^AATTC
HaeII 2048 GGCGCC [GA]GCGC^[CT]
HgaI 3379 GCGTC GACGC(5/10)
KasI 2048 GGCGCC G^GCGCC
KpnI 729 GGTACC GGTAC^C
MamI 1436 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1107 TCCGGA T^CCGGA
NarI 2048 GGCGCC GG^CGCC
NheI 3474 GCTAGC G^CTAGC
PflFI 287 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SexAI 5396 ACCTGGT A^CC[AT]GGT
SfoI 2048 GGCGCC GGC^GCC
SnaBI 4977 TACGTA TAC^GTA
SpeI 4586 ACTAGT A^CTAGT
Tth111I 287 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AspHI 976 GAGCAC G[AT]GC[AT]^C
5083 GTGCTC
BbvCI 2659 CCTCAGC CCTCAGC(-5/-2)
3234 CCTCAGC
BglII 355 AGATCT A^GATCT
4982 AGATCT
BsiHKAI 976 GAGCAC G[AT]GC[AT]^C
5083 GTGCTC
BspHI 3787 TCATGA T^CATGA
3872 TCATGA
BsrDI 1988 CATTGC GCAATG(2/0)
3174 GCAATG
CfoI 2049 GCGC GCG^C
2689 GCGC
EcoRV 3107 GATATC GAT^ATC
4778 GATATC
HapII 416 CCGG C^CGG
1108 CCGG
HhaI 2049 GCGC GCG^C
2689 GCGC
HinP1I 2049 GCGC G^CGC
2689 GCGC
HincII 293 GTCAAC GT[CT]^[GA]AC
4764 GTTGAC
HindII 293 GTCAAC GT[CT]^[GA]AC
4764 GTTGAC
HindIII 1112 AAGCTT A^AGCTT
1834 AAGCTT
HpaII 416 CCGG C^CGG
1108 CCGG
MspI 416 CCGG C^CGG
1108 CCGG
PflMI 454 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2827 CCATTGTGTGG
PpuMI 348 AGGTCCC [GA]G^G[AT]CC[CT]
1606 GGGTCCT
RcaI 3787 TCATGA T^CATGA
3872 TCATGA
SapI 250 GAAGAGC GCTCTTC(1/4)
641 GAAGAGC
StuI 7 AGGCCT AGG^CCT
520 AGGCCT
Van91I 454 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2827 CCATTGTGTGG
XcmI 775 CCATACCATTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1176 CCAATGCTAATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApaI ApoI Asp700I
AvaII BalI BanI BanII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmBI BsmFI
Bsp1286I Bsp1407I BspMI BsrGI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
Hin1I HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MluNI MnlI MscI
MseI MslI MspA1I MunI MvaI
MwoI NdeI NdeII NlaIII NlaIV
NspI PleI PstI RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SphI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XhoII XmnI