##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII AgeI AscI AseI AsnI
Asp700I BbvCI BcgI BclI BsaBI
BsiEI BsiWI BsmBI BsmI BspDI
BspLU11I BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraIII EagI EclXI
Eco52I EcoRV FbaI FseI HpaI
KspI MamI MfeI MluI MunI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshBI PvuI
RsrII SacII SalI SexAI SfiI
SfuI SgrAI SnaBI SpeI SwaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 2462 GGTACC G^GTACC
AccIII 4530 TCCGGA T^CCGGA
AclI 946 AACGTT AA^CGTT
AflII 2789 CTTAAG C^TTAAG
AflIII 4549 ACACGT A^C[GA][CT]GT
Alw44I 847 GTGCAC G^TGCAC
Aor51HI 134 AGCGCT AGC^GCT
ApaLI 847 GTGCAC G^TGCAC
Asp718I 2462 GGTACC G^GTACC
BbeI 928 GGCGCC GGCGC^C
BciVI 2267 GGATAC GTATCC(6/5)
BfrI 2789 CTTAAG C^TTAAG
BglII 2995 AGATCT A^GATCT
BlpI 1198 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1198 GCTGAGC GC^T[ACGT]AGC
BsaWI 4530 TCCGGA [AT]^CCGG[AT]
BseAI 4530 TCCGGA T^CCGGA
BspEI 4530 TCCGGA T^CCGGA
BspHI 1697 TCATGA T^CATGA
BstEII 1563 GGTGACC G^GT[ACGT]ACC
BstPI 1563 GGTGACC G^GT[ACGT]ACC
CelII 1198 GCTGAGC GC^T[ACGT]AGC
DraI 3946 TTTAAA TTT^AAA
DrdI 1006 GACTTCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 134 AGCGCT AGC^GCT
EcoO65I 1563 GGTGACC G^GT[ACGT]ACC
EcoRI 1435 GAATTC G^AATTC
EcoT22I 4853 ATGCAT ATGCA^T
HindIII 4899 AAGCTT A^AGCTT
KasI 928 GGCGCC G^GCGCC
KpnI 2462 GGTACC GGTAC^C
MroI 4530 TCCGGA T^CCGGA
NarI 928 GGCGCC GG^CGCC
NdeI 3973 CATATG CA^TATG
NsiI 4853 ATGCAT ATGCA^T
Psp1406I 946 AACGTT AA^CGTT
RcaI 1697 TCATGA T^CATGA
SbfI 1986 CCTGCAGG CCTGCA^GG
SfoI 928 GGCGCC GGC^GCC
Sse8387I 1986 CCTGCAGG CCTGCA^GG
SspI 3033 AATATT AAT^ATT
XbaI 4795 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccI 1015 GTCTAC GT^[AC][GT]AC
2613 GTAGAC
AhdI 166 GACACCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
325 GACAATGAGTC
ApaI 100 GGGCCC GGGCC^C
2063 GGGCCC
AspEI 166 GACACCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
325 GACAATGAGTC
AspI 451 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4054 GACTCAGTC
AvrII 2486 CCTAGG C^CTAGG
3716 CCTAGG
BbrPI 2399 CACGTG CAC^GTG
2417 CACGTG
BbsI 2481 GTCTTC GAAGAC(2/6)
4636 GTCTTC
BglI 499 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4236 GCCTAGCAGGC
BlnI 2486 CCTAGG C^CTAGG
3716 CCTAGG
BpuAI 2481 GTCTTC GAAGAC(2/6)
4636 GTCTTC
BsaAI 2399 CACGTG [CT]AC^GT[GA]
2417 CACGTG
BsgI 808 GTGCAG GTGCAG(16/14)
4046 GTGCAG
Bsp1407I 2622 TGTACA T^GTACA
4979 TGTACA
BsrDI 3624 CATTGC GCAATG(2/0)
3150 GCAATG
BsrGI 2622 TGTACA T^GTACA
4979 TGTACA
BssSI 338 CACGAG CACGAG(-5/-1)
736 CTCGTG
BstAPI 3009 GCAAAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4666 GCAGTATCTGC
Eam1105I 166 GACACCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
325 GACAATGAGTC
HaeII 134 AGCGCT [GA]GCGC^[CT]
928 GGCGCC
HincII 40 GTTGAC GT[CT]^[GA]AC
1928 GTCAAC
HindII 40 GTTGAC GT[CT]^[GA]AC
1928 GTCAAC
NaeI 668 GCCGGC GCC^GGC
2763 GCCGGC
NgoMIV 668 GCCGGC G^CCGGC
2763 GCCGGC
PflFI 451 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4054 GACTCAGTC
PmaCI 2399 CACGTG CAC^GTG
2417 CACGTG
PmlI 2399 CACGTG CAC^GTG
2417 CACGTG
PshAI 1165 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3615 GACTGGGGTC
PvuII 648 CAGCTG CAG^CTG
4049 CAGCTG
ScaI 179 AGTACT AGT^ACT
1181 AGTACT
SmaI 307 CCCGGG CCC^GGG
1322 CCCGGG
SphI 3381 GCATGC GCATG^C
4851 GCATGC
SspBI 2622 TGTACA T^GTACA
4979 TGTACA
TaqI 264 TCGA T^CGA
2722 TCGA
Tth111I 451 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4054 GACTCAGTC
TthHB8I 264 TCGA T^CGA
2722 TCGA
XmaCI 307 CCCGGG C^CCGGG
1322 CCCGGG
XmaI 307 CCCGGG C^CCGGG
1322 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
AspHI AvaI AvaII AviII BalI
BamHI BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XcmI
XhoII