##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Alw44I ApaI ApaLI AscI
Asp718I AspEI AspI AviII AvrII
BbeI BbrPI BbvCI BfrI BglI
BlnI BsaAI BseAI BsiWI BspDI
BspEI BsrBI BsrFI BssHII BstAPI
BstEII BstPI Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRV FseI FspI
HindIII HpaI KasI KpnI KspI
MluI MroI NaeI NarI NgoMIV
NheI NotI NruI PacI PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI SphI Sse8387I
Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 3690 GACGTC GACGT^C
AccI 3901 GTATAC GT^[AC][GT]AC
AocI 2404 CCTCAGG CC^T[ACGT]AGG
AvaI 103 CTCGAG C^[CT]CG[GA]G
BalI 2765 TGGCCA TGG^CCA
BglII 564 AGATCT A^GATCT
BlpI 404 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 404 GCTTAGC GC^T[ACGT]AGC
BsgI 3184 CTGCAC GTGCAG(16/14)
BsiEI 3271 CGACCG CG[GA][CT]^CG
BsmBI 163 GAGACG CGTCTC(1/5)
BsmFI 3413 GTCCC GGGAC(10/14)
BsoBI 103 CTCGAG C^[CT]CG[GA]G
BspMI 5004 GCAGGT ACCTGC(4/8)
BssSI 3323 CACGAG CACGAG(-5/-1)
Bst1107I 3901 GTATAC GTA^TAC
BstBI 184 TTCGAA TT^CGAA
BstZ17I 3901 GTATAC GTA^TAC
Bsu36I 2404 CCTCAGG CC^T[ACGT]AGG
CelII 404 GCTTAGC GC^T[ACGT]AGC
EaeI 2765 TGGCCA [CT]^GGCC[GA]
Eco81I 2404 CCTCAGG CC^T[ACGT]AGG
EcoNI 910 CCTTTGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3883 GAATTC G^AATTC
FauI 142 GCGGG CCCGC(4/6)
MfeI 3602 CAATTG C^AATTG
MluNI 2765 TGGCCA TGG^CCA
MscI 2765 TGGCCA TGG^CCA
MunI 3602 CAATTG C^AATTG
NspV 184 TTCGAA TT^CGAA
PaeR7I 103 CTCGAG C^TCGAG
PflMI 1065 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 1350 GCTCTTC GCTCTTC(1/4)
ScaI 3858 AGTACT AGT^ACT
SfuI 184 TTCGAA TT^CGAA
SpeI 3381 ACTAGT A^CTAGT
SspI 4680 AATATT AAT^ATT
StuI 918 AGGCCT AGG^CCT
SwaI 4019 ATTTAAAT ATTT^AAAT
Van91I 1065 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4746 TCTAGA T^CTAGA
XhoI 103 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 2970 CGCG CG^CG
3214 CGCG
AcyI 41 GACGCC G[GA]^CG[CT]C
3690 GACGTC
AflIII 4480 ACATGT A^C[GA][CT]GT
4659 ACATGT
Aor51HI 23 AGCGCT AGC^GCT
1043 AGCGCT
Asp700I 1347 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3856 GAAGTACTTC
BcgI 2094 CGAGAGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3377 GCAGACTAGTCG
BclI 3611 TGATCA T^GATCA
4539 TGATCA
BcnI 115 CCGGG CC^[GC]GG
125 CCGGG
BsaBI 934 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4725 GATTTATATC
BsaHI 41 GACGCC G[GA]^CG[CT]C
3690 GACGTC
BsaWI 1988 ACCGGA [AT]^CCGG[AT]
3093 TCCGGT
Bsp1407I 627 TGTACA T^GTACA
2991 TGTACA
BspLU11I 4480 ACATGT A^CATGT
4659 ACATGT
BsrGI 627 TGTACA T^GTACA
2991 TGTACA
BstF5I 1991 GGATG GGATG(2/0)
3593 GGATG
BstUI 2970 CGCG CG^CG
3214 CGCG
DsaI 1091 CCATGG C^C[GA][CT]GG
1631 CCATGG
Eco47III 23 AGCGCT AGC^GCT
1043 AGCGCT
FbaI 3611 TGATCA T^GATCA
4539 TGATCA
FokI 1991 GGATG GGATG(9/13)
3593 GGATG
HaeII 23 AGCGCT [GA]GCGC^[CT]
1043 AGCGCT
HgaI 41 GACGC GACGC(5/10)
165 GACGC
Hin1I 41 GACGCC G[GA]^CG[CT]C
3690 GACGTC
MaeII 3691 ACGT A^CGT
3781 ACGT
MamI 934 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4725 GATTTATATC
MspA1I 3458 CAGCTG C[AC]G^C[GT]G
4178 CAGCTG
MvnI 2970 CGCG CG^CG
3214 CGCG
NciI 115 CCGGG CC^[GC]GG
125 CCGGG
NcoI 1091 CCATGG C^CATGG
1631 CCATGG
NdeI 929 CATATG CA^TATG
1210 CATATG
PvuII 3458 CAGCTG CAG^CTG
4178 CAGCTG
SspBI 627 TGTACA T^GTACA
2991 TGTACA
XmnI 1347 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3856 GAAGTACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI AspHI
AvaII BamHI BanI BanII BbsI
BbvI BciVI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmI Bsp1286I
BspHI BsrDI BsrI BssKI BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EarI
EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NdeII NlaIII NlaIV
NsiI NspI PleI PshBI PstI
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII