##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
Acc65I AclI AgeI Alw44I ApaLI
AscI Asp700I Asp718I BbeI BclI
BglI BlpI Bpu1102I BsaBI BsiWI
BstAPI BstBI CelII CpoI DrdI
EcoRV FbaI FseI HindIII HpaI
KasI KpnI MamI MfeI MluI
MunI NaeI NarI NgoMIV NotI
NruI NspV PacI PflMI PinAI
PmeI Psp1406I PvuI RsrII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI Van91I XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccIII 1943 TCCGGA T^CCGGA
AflII 4905 CTTAAG C^TTAAG
AhdI 2674 GACAGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 6039 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2009 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2145 AGCGCT AGC^GCT
AspEI 2674 GACAGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 972 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 773 CCTAGG C^CTAGG
BalI 3307 TGGCCA TGG^CCA
BbrPI 3303 CACGTG CAC^GTG
BcgI 4006 CGAGCAGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4905 CTTAAG C^TTAAG
BlnI 773 CCTAGG C^CTAGG
BsaAI 3303 CACGTG [CT]AC^GT[GA]
BseAI 1943 TCCGGA T^CCGGA
BspDI 4271 ATCGAT AT^CGAT
BspEI 1943 TCCGGA T^CCGGA
Bst1107I 5463 GTATAC GTA^TAC
BstZ17I 5463 GTATAC GTA^TAC
Bsu36I 2009 CCTGAGG CC^T[ACGT]AGG
ClaI 4271 ATCGAT AT^CGAT
EagI 305 CGGCCG C^GGCCG
Eam1105I 2674 GACAGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 305 CGGCCG C^GGCCG
Eco47III 2145 AGCGCT AGC^GCT
Eco52I 305 CGGCCG C^GGCCG
Eco81I 2009 CCTGAGG CC^T[ACGT]AGG
EcoNI 4597 CCTCTTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1555 GAATTC G^AATTC
HincII 1091 GTCAAC GT[CT]^[GA]AC
HindII 1091 GTCAAC GT[CT]^[GA]AC
MluNI 3307 TGGCCA TGG^CCA
MroI 1943 TCCGGA T^CCGGA
MscI 3307 TGGCCA TGG^CCA
NdeI 1902 CATATG CA^TATG
NheI 5280 GCTAGC G^CTAGC
PaeR7I 117 CTCGAG C^TCGAG
PflFI 972 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3303 CACGTG CAC^GTG
PmlI 3303 CACGTG CAC^GTG
PpuMI 502 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 2999 GACAGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 3233 GAGCTC GAGCT^C
SexAI 5424 ACCTGGT A^CC[AT]GGT
SspI 5037 AATATT AAT^ATT
StuI 2095 AGGCCT AGG^CCT
Tth111I 972 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5171 TCTAGA T^CTAGA
XhoI 117 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AatII 995 GACGTC GACGT^C
3003 GACGTC
AcyI 995 GACGTC G[GA]^CG[CT]C
3003 GACGTC
AseI 4741 ATTAAT AT^TAAT
5928 ATTAAT
AsnI 4741 ATTAAT AT^TAAT
5928 ATTAAT
BbvCI 2485 CCTCAGC CCTCAGC(-5/-2)
2588 GCTGAGG
BciVI 6019 GGATAC GTATCC(6/5)
5624 GTATCC
BglII 1113 AGATCT A^GATCT
5114 AGATCT
BsaHI 995 GACGTC G[GA]^CG[CT]C
3003 GACGTC
BsaWI 1855 TCCGGT [AT]^CCGG[AT]
1943 TCCGGA
BsiEI 305 CGGCCG CG[GA][CT]^CG
976 CGGTCG
BsmI 684 GAATGC GAATGC(1/-1)
808 GAATGC
BspHI 2714 TCATGA T^CATGA
3562 TCATGA
BsrBI 1574 CCGCTC CCGCTC(-3/-3)
299 GAGCGG
BsrFI 3786 ACCGGC [GA]^CCGG[CT]
5608 ACCGGC
BssHII 11 GCGCGC G^CGCGC
234 GCGCGC
Cfr10I 3786 ACCGGC [GA]^CCGG[CT]
5608 ACCGGC
DraIII 3264 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
3429 CACAGTGTG
EcoT22I 1899 ATGCAT ATGCA^T
5581 ATGCAT
Hin1I 995 GACGTC G[GA]^CG[CT]C
3003 GACGTC
KspI 105 CCGCGG CCGC^GG
428 CCGCGG
NsiI 1899 ATGCAT ATGCA^T
5581 ATGCAT
PshBI 4741 ATTAAT AT^TAAT
5928 ATTAAT
RcaI 2714 TCATGA T^CATGA
3562 TCATGA
SacII 105 CCGCGG CCGC^GG
428 CCGCGG
SmaI 258 CCCGGG CCC^GGG
347 CCCGGG
XmaCI 258 CCCGGG C^CCGGG
347 CCCGGG
XmaI 258 CCCGGG C^CCGGG
347 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI ApaI ApoI
AspHI AvaI AvaII AviII BamHI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I Bsp1407I BspLU11I BspMI BsrDI
BsrGI BsrI BssKI BssSI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII