##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AhdI Aor51HI ApaI AscI Asp718I
AspEI AspI AviII AvrII BbeI
BbrPI BcgI BciVI BclI BfrI
BlnI BsaAI BsaI BsiEI BsiWI
BspDI BspHI BspLU11I BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV FbaI FseI FspI HindIII
KasI KpnI MluI NarI NdeI
NheI NotI NruI NspV PflFI
PmaCI PmeI PmlI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SfiI SfoI SfuI SnaBI Sse8387I
SspI StuI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccIII 154 TCCGGA T^CCGGA
AcyI 194 GACGCC G[GA]^CG[CT]C
AseI 3966 ATTAAT AT^TAAT
AsnI 3966 ATTAAT AT^TAAT
BalI 304 TGGCCA TGG^CCA
BbvCI 1810 CCTCAGC CCTCAGC(-5/-2)
BglI 5349 GCCATAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 994 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 994 GCTAAGC GC^T[ACGT]AGC
BsaBI 4183 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 194 GACGCC G[GA]^CG[CT]C
BseAI 154 TCCGGA T^CCGGA
BspEI 154 TCCGGA T^CCGGA
BsrBI 30 GAGCGG CCGCTC(-3/-3)
BssSI 1111 CACGAG CACGAG(-5/-1)
CelII 994 GCTAAGC GC^T[ACGT]AGC
DraIII 2166 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I 5390 ATGCAT ATGCA^T
HaeII 1763 AGCGCC [GA]GCGC^[CT]
HgaI 194 GACGC GACGC(5/10)
Hin1I 194 GACGCC G[GA]^CG[CT]C
HpaI 2489 GTTAAC GTT^AAC
KspI 2217 CCGCGG CCGC^GG
MamI 4183 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4148 CAATTG C^AATTG
MluNI 304 TGGCCA TGG^CCA
MroI 154 TCCGGA T^CCGGA
MscI 304 TGGCCA TGG^CCA
MunI 4148 CAATTG C^AATTG
NaeI 1141 GCCGGC GCC^GGC
NgoMIV 1141 GCCGGC G^CCGGC
NsiI 5390 ATGCAT ATGCA^T
NspI 2993 GCATGC [GA]CATG^[CT]
PacI 758 TTAATTAA TTAAT^TAA
PshAI 2261 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3966 ATTAAT AT^TAAT
SacI 1174 GAGCTC GAGCT^C
SacII 2217 CCGCGG CCGC^GG
SexAI 357 ACCAGGT A^CC[AT]GGT
SgrAI 2472 CACCGGTG C[GA]^CCGG[CT]G
SmaI 1068 CCCGGG CCC^GGG
SpeI 466 ACTAGT A^CTAGT
SphI 2993 GCATGC GCATG^C
XcmI 3356 CCAGAACAGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1068 CCCGGG C^CCGGG
XmaI 1068 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 417 GTAGAC GT^[AC][GT]AC
3076 GTCTAC
AccII 2218 CGCG CG^CG
2242 CGCG
AgeI 1318 ACCGGT A^CCGGT
2473 ACCGGT
AocI 2187 CCTCAGG CC^T[ACGT]AGG
3133 CCTCAGG
BamHI 940 GGATCC G^GATCC
3127 GGATCC
BglII 807 AGATCT A^GATCT
5328 AGATCT
BsmBI 742 CGTCTC CGTCTC(1/5)
2040 CGTCTC
Bsp1407I 4667 TGTACA T^GTACA
4693 TGTACA
BspMI 977 ACCTGC ACCTGC(4/8)
2509 GCAGGT
BsrGI 4667 TGTACA T^GTACA
4693 TGTACA
BstEII 1328 GGTGACC G^GT[ACGT]ACC
3572 GGTGACC
BstPI 1328 GGTGACC G^GT[ACGT]ACC
3572 GGTGACC
BstUI 2218 CGCG CG^CG
2242 CGCG
BstXI 1422 CCAGACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4143 CCACACAATTGG
Bsu36I 2187 CCTCAGG CC^T[ACGT]AGG
3133 CCTCAGG
Eco81I 2187 CCTCAGG CC^T[ACGT]AGG
3133 CCTCAGG
EcoO65I 1328 GGTGACC G^GT[ACGT]ACC
3572 GGTGACC
FauI 2216 CCCGC CCCGC(4/6)
2455 CCCGC
HincII 2489 GTTAAC GT[CT]^[GA]AC
5111 GTCAAC
HindII 2489 GTTAAC GT[CT]^[GA]AC
5111 GTCAAC
MaeII 3074 ACGT A^CGT
4361 ACGT
MvnI 2218 CGCG CG^CG
2242 CGCG
PaeR7I 1687 CTCGAG C^TCGAG
2386 CTCGAG
PinAI 1318 ACCGGT A^CCGGT
2473 ACCGGT
PvuII 3313 CAGCTG CAG^CTG
3580 CAGCTG
SspBI 4667 TGTACA T^GTACA
4693 TGTACA
XhoI 1687 CTCGAG C^TCGAG
2386 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI AlwNI ApaLI ApoI Asp700I
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI