##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AclI AgeI Aor51HI AscI AviII
AvrII BamHI BglI BlnI BsmBI
Bsp1407I BspDI BsrGI BssHII BssSI
BstAPI BstBI ClaI Eco47III EcoRV
FspI KspI MfeI MluI MunI
NotI NruI NspV PacI PinAI
PmeI PshAI Psp1406I PvuI SacII
SbfI SexAI SfiI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 1520 GACGTC GACGT^C
Acc65I 420 GGTACC G^GTACC
AccIII 4480 TCCGGA T^CCGGA
AhdI 6124 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 234 GGGCCC GGGCC^C
Asp718I 420 GGTACC G^GTACC
AspEI 6124 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4668 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 5078 CACGTG CAC^GTG
BbvCI 1824 GCTGAGG CCTCAGC(-5/-2)
BlpI 7017 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 7017 GCTTAGC GC^T[ACGT]AGC
BsaAI 5078 CACGTG [CT]AC^GT[GA]
BsaBI 8641 GATTCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 4480 TCCGGA T^CCGGA
BsiWI 8073 CGTACG C^GTACG
BspEI 4480 TCCGGA T^CCGGA
BspHI 7674 TCATGA T^CATGA
BsrBI 1229 GAGCGG CCGCTC(-3/-3)
Bst1107I 5980 GTATAC GTA^TAC
BstEII 7682 GGTTACC G^GT[ACGT]ACC
BstPI 7682 GGTTACC G^GT[ACGT]ACC
BstZ17I 5980 GTATAC GTA^TAC
CelII 7017 GCTTAGC GC^T[ACGT]AGC
CpoI 122 CGGACCG CG^G[AT]CCG
DraIII 3016 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 6124 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 7682 GGTTACC G^GT[ACGT]ACC
FseI 138 GGCCGGCC GGCCGG^CC
HgaI 10 GACGC GACGC(5/10)
HindIII 3279 AAGCTT A^AGCTT
HpaI 1602 GTTAAC GTT^AAC
KpnI 420 GGTACC GGTAC^C
MamI 8641 GATTCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 4480 TCCGGA T^CCGGA
PaeR7I 64 CTCGAG C^TCGAG
PflFI 4668 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 5078 CACGTG CAC^GTG
PmlI 5078 CACGTG CAC^GTG
PpuMI 712 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 7674 TCATGA T^CATGA
RsrII 122 CGGACCG CG^G[AT]CCG
SalI 6121 GTCGAC G^TCGAC
StuI 7668 AGGCCT AGG^CCT
SwaI 3326 ATTTAAAT ATTT^AAAT
Tth111I 4668 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 64 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 173 CGCG CG^CG
180 CGCG
Alw44I 1009 GTGCAC G^TGCAC
2702 GTGCAC
ApaLI 1009 GTGCAC G^TGCAC
2702 GTGCAC
AvaI 64 CTCGAG C^[CT]CG[GA]G
760 CCCGAG
BcgI 1073 CGATGAAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2557 GCATTCATCTCG
BciVI 6942 GGATAC GTATCC(6/5)
1256 GTATCC
BsiEI 137 CGGCCG CG[GA][CT]^CG
203 CGGCCG
BsoBI 64 CTCGAG C^[CT]CG[GA]G
760 CCCGAG
BstUI 173 CGCG CG^CG
180 CGCG
DraII 234 GGGCCCC [GA]G^G[ACGT]CC[CT]
712 AGGTCCT
DrdI 6858 GACTTCCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7991 GACATTGGAGTC
EagI 137 CGGCCG C^GGCCG
203 CGGCCG
EclXI 137 CGGCCG C^GGCCG
203 CGGCCG
Eco52I 137 CGGCCG C^GGCCG
203 CGGCCG
EcoO109I 234 GGGCCCC [GA]G^G[ACGT]CC[CT]
712 AGGTCCT
EcoRI 3582 GAATTC G^AATTC
4156 GAATTC
MvnI 173 CGCG CG^CG
180 CGCG
NdeI 1039 CATATG CA^TATG
7168 CATATG
SacI 2628 GAGCTC GAGCT^C
4558 GAGCTC
SapI 1819 GAAGAGC GCTCTTC(1/4)
2221 GCTCTTC
SpeI 1557 ACTAGT A^CTAGT
7030 ACTAGT
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflII AflIII AluI AlwI AlwNI
AocI ApoI AseI AsnI Asp700I
AspHI AvaII BalI BanI BanII
BbeI BbsI BbvI BclI BcnI
BfaI BfrI BglII BmyI BpmI
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspLU11I
BspMI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DsaI EaeI EarI
Eco81I EcoNI EcoRII EcoT14I EcoT22I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NheI
NlaIII NlaIV NsiI NspI PflMI
PleI PshBI PstI PvuII RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SfoI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XbaI
XcmI XhoII XmnI