##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII AccII AccIII AclI AgeI
AhdI Aor51HI ApaI AscI Asp700I
AspEI AspI AvaI AviII AvrII
BalI BanII BbeI BbrPI BcgI
BcnI BlnI BlpI Bpu1102I BsaAI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRV FauI FseI
FspI HaeII HapII HhaI HinP1I
HpaII KasI KspI MluI MluNI
MroI MscI MspI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmlI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I Tth111I XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 1376 GGTACC G^GTACC
AcyI 2243 GACGCC G[GA]^CG[CT]C
AflII 124 CTTAAG C^TTAAG
Alw44I 1344 GTGCAC G^TGCAC
AlwNI 3279 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 600 CCTCAGG CC^T[ACGT]AGG
ApaLI 1344 GTGCAC G^TGCAC
Asp718I 1376 GGTACC G^GTACC
BamHI 643 GGATCC G^GATCC
BanI 1376 GGTACC G^G[CT][GA]CC
BbvCI 2672 CCTCAGC CCTCAGC(-5/-2)
BciVI 4139 GGATAC GTATCC(6/5)
BfrI 124 CTTAAG C^TTAAG
BglI 1491 GCCATCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3587 AGATCT A^GATCT
BsaHI 2243 GACGCC G[GA]^CG[CT]C
BsgI 5016 GTGCAG GTGCAG(16/14)
BsiYI 3746 CCACGGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 3746 CCACGGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 1196 GTCCC GGGAC(10/14)
BsrBI 25 GAGCGG CCGCTC(-3/-3)
BstXI 2599 CCAAAACGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 600 CCTCAGG CC^T[ACGT]AGG
DraII 3679 AGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 2731 GACTGATTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 3746 CCACGG C^C[GA][CT]GG
Eco81I 600 CCTCAGG CC^T[ACGT]AGG
EcoO109I 3679 AGGACCC [GA]G^G[ACGT]CC[CT]
HgaI 2243 GACGC GACGC(5/10)
Hin1I 2243 GACGCC G[GA]^CG[CT]C
HindIII 2260 AAGCTT A^AGCTT
KpnI 1376 GGTACC GGTAC^C
MfeI 1381 CAATTG C^AATTG
MunI 1381 CAATTG C^AATTG
PflMI 3746 CCACGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 4453 GTTTAAAC GTTT^AAAC
PpuMI 3679 AGGACCC [GA]G^G[AT]CC[CT]
PstI 2688 CTGCAG CTGCA^G
StuI 2252 AGGCCT AGG^CCT
SwaI 4890 ATTTAAAT ATTT^AAAT
Van91I 3746 CCACGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AspHI 162 GTGCTC G[AT]GC[AT]^C
1344 GTGCAC
AvaII 218 GGACC G^G[AT]CC
3680 GGACC
BclI 2112 TGATCA T^GATCA
4829 TGATCA
BmyI 162 GTGCTC G[AGT]GC[ACT]^C
1344 GTGCAC
BsaBI 2345 GATAGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2507 GATATTAATC
BsiHKAI 162 GTGCTC G[AT]GC[AT]^C
1344 GTGCAC
BsmI 1487 GAATGC GAATGC(1/-1)
1690 GCATTC
Bsp1286I 162 GTGCTC G[AGT]GC[ACT]^C
1344 GTGCAC
Bsp1407I 1100 TGTACA T^GTACA
4481 TGTACA
BspHI 1578 TCATGA T^CATGA
5031 TCATGA
BspLU11I 1956 ACATGT A^CATGT
4105 ACATGT
BsrGI 1100 TGTACA T^GTACA
4481 TGTACA
Cfr13I 218 GGACC G^G[ACGT]CC
3680 GGACC
EaeI 1904 CGGCCA [CT]^GGCC[GA]
3831 TGGCCG
EcoRI 481 GAATTC G^AATTC
3418 GAATTC
EcoT22I 885 ATGCAT ATGCA^T
5184 ATGCAT
FbaI 2112 TGATCA T^GATCA
4829 TGATCA
HpaI 4074 GTTAAC GTT^AAC
4668 GTTAAC
MamI 2345 GATAGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2507 GATATTAATC
NsiI 885 ATGCAT ATGCA^T
5184 ATGCAT
PshAI 2374 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3681 GACCCTTGTC
PvuII 339 CAGCTG CAG^CTG
350 CAGCTG
RcaI 1578 TCATGA T^CATGA
5031 TCATGA
SapI 854 GAAGAGC GCTCTTC(1/4)
1079 GAAGAGC
Sau96I 218 GGACC G^G[ACGT]CC
3680 GGACC
ScaI 1683 AGTACT AGT^ACT
4368 AGTACT
SmlI 124 CTTAAG C^T[CT][GA]AG
2744 CTTGAG
SspBI 1100 TGTACA T^GTACA
4481 TGTACA
SspI 4515 AATATT AAT^ATT
4862 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI AseI AsnI
BbsI BbvI BfaI BpmI BpuAI
BsaJI BseRI BsmAI BspMI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I DdeI DpnII DraI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HincII HindII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MwoI NdeI NdeII
NlaIII NlaIV NspI PleI PshBI
RsaI Sau3AI ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XhoII