##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
Acc65I AccII AccIII AgeI AhdI
AocI Aor51HI ApaI AscI Asp718I
AspEI AspI AviII AvrII BbrPI
BcgI BcnI BlnI BlpI Bpu1102I
BsaAI BsaWI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BsrBI BssHII
BssSI BstBI BstEII BstPI BstUI
BstXI Bsu36I CelII ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT22I FseI FspI HindIII KpnI
KspI MluI MroI MvnI NciI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PvuI RsrII SacII
SalI SbfI SexAI SfiI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SwaI Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 2195 GACGTC GACGT^C
AccI 3421 GTATAC GT^[AC][GT]AC
AclI 4011 AACGTT AA^CGTT
Alw44I 4769 GTGCAC G^TGCAC
ApaLI 4769 GTGCAC G^TGCAC
Asp700I 4976 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1588 CTCGAG C^[CT]CG[GA]G
BalI 889 TGGCCA TGG^CCA
BamHI 2615 GGATCC G^GATCC
BanI 766 GGCGCC G^G[CT][GA]CC
BbeI 766 GGCGCC GGCGC^C
BclI 1243 TGATCA T^GATCA
BglI 1513 GCCAAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 4361 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 2393 GAATGC GAATGC(1/-1)
BsoBI 1588 CTCGAG C^[CT]CG[GA]G
Bsp1407I 118 TGTACA T^GTACA
BsrDI 3938 CATTGC GCAATG(2/0)
BsrFI 2348 GCCGGC [GA]^CCGG[CT]
BsrGI 118 TGTACA T^GTACA
Bst1107I 3421 GTATAC GTA^TAC
BstAPI 2378 GCATTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 3421 GTATAC GTA^TAC
Cfr10I 2348 GCCGGC [GA]^CCGG[CT]
DraII 863 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 1164 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 889 TGGCCA [CT]^GGCC[GA]
EcoO109I 863 AGGACCT [GA]G^G[ACGT]CC[CT]
FbaI 1243 TGATCA T^GATCA
HapII 2349 CCGG C^CGG
HpaII 2349 CCGG C^CGG
KasI 766 GGCGCC G^GCGCC
MamI 4361 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2629 CAATTG C^AATTG
MluNI 889 TGGCCA TGG^CCA
MscI 889 TGGCCA TGG^CCA
MspI 2349 CCGG C^CGG
MunI 2629 CAATTG C^AATTG
NaeI 2348 GCCGGC GCC^GGC
NarI 766 GGCGCC GG^CGCC
NgoMIV 2348 GCCGGC G^CCGGC
PaeR7I 1588 CTCGAG C^TCGAG
PpuMI 863 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1710 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4011 AACGTT AA^CGTT
PvuII 397 CAGCTG CAG^CTG
SacI 4995 GAGCTC GAGCT^C
SapI 2484 GCTCTTC GCTCTTC(1/4)
SfoI 766 GGCGCC GGC^GCC
SpeI 2543 ACTAGT A^CTAGT
SspBI 118 TGTACA T^GTACA
TaqI 1589 TCGA T^CGA
TthHB8I 1589 TCGA T^CGA
XhoI 1588 CTCGAG C^TCGAG
XmnI 4976 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AvaII 864 GGACC G^G[AT]CC
2042 GGACC
BciVI 1475 GGATAC GTATCC(6/5)
4112 GGATAC
BglII 2669 AGATCT A^GATCT
4762 AGATCT
BsgI 1902 GTGCAG GTGCAG(16/14)
3500 GTGCAG
BspHI 4163 TCATGA T^CATGA
5321 TCATGA
BspLU11I 925 ACATGT A^CATGT
2636 ACATGT
BspMI 27 GCAGGT ACCTGC(4/8)
1907 GCAGGT
DsaI 1480 CCATGG C^C[GA][CT]GG
3005 CCATGG
FauI 2766 CCCGC CCCGC(4/6)
701 GCGGG
HgaI 1615 GACGC GACGC(5/10)
3962 GCGTC
MslI 2573 CACCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4054 CACCATTATG
NcoI 1480 CCATGG C^CATGG
3005 CCATGG
RcaI 4163 TCATGA T^CATGA
5321 TCATGA
ScaI 247 AGTACT AGT^ACT
2681 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflII
AflIII AluI AlwI AlwNI ApoI
AseI AsnI AspHI BanII BbsI
BbvCI BbvI BfaI BfrI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EarI EcoNI EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HhaI Hin1I HinP1I HincII
HindII HinfI HpaI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI PshBI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XbaI XcmI XhoII