##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII AccII AccIII AcyI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AspEI AspI AvaI AviII
AvrII BbeI BbrPI BcgI BcnI
BlnI BlpI Bpu1102I BsaHI BsaWI
BseAI BsiWI BsmBI BsmFI BsoBI
BspDI BspEI BsrBI BsrFI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HpaII KasI
KspI MluI MroI MspI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I Tth111I XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 33 GGTACC G^GTACC
AccI 47 GTCTAC GT^[AC][GT]AC
AclI 246 AACGTT AA^CGTT
Asp718I 33 GGTACC G^GTACC
BalI 5065 TGGCCA TGG^CCA
BamHI 3861 GGATCC G^GATCC
BciVI 2436 GTATCC GTATCC(6/5)
BglI 3663 GCCTCTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2562 AGATCT A^GATCT
BsaAI 4723 TACGTG [CT]AC^GT[GA]
BsiEI 3186 CGACCG CG[GA][CT]^CG
BsmI 3540 GCATTC GAATGC(1/-1)
BspHI 2166 TCATGA T^CATGA
BspMI 2772 GCAGGT ACCTGC(4/8)
BssSI 3578 CTCGTG CACGAG(-5/-1)
BstAPI 256 GCATCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 2967 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 5065 TGGCCA [CT]^GGCC[GA]
EcoNI 3808 CCTAGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1445 GATATC GAT^ATC
HpaI 1765 GTTAAC GTT^AAC
KpnI 33 GGTACC GGTAC^C
MluNI 5065 TGGCCA TGG^CCA
MscI 5065 TGGCCA TGG^CCA
PacI 5722 TTAATTAA TTAAT^TAA
PpuMI 5265 GGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 246 AACGTT AA^CGTT
RcaI 2166 TCATGA T^CATGA
SacI 4813 GAGCTC GAGCT^C
SpeI 3499 ACTAGT A^CTAGT
SwaI 5015 ATTTAAAT ATTT^AAAT
XbaI 3733 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AflII 969 CTTAAG C^TTAAG
1471 CTTAAG
AocI 3673 CCTCAGG CC^T[ACGT]AGG
3706 CCTCAGG
BanI 33 GGTACC G^G[CT][GA]CC
1416 GGCACC
BbvCI 2674 CCTCAGC CCTCAGC(-5/-2)
3019 CCTCAGC
BclI 987 TGATCA T^GATCA
2760 TGATCA
BfrI 969 CTTAAG C^TTAAG
1471 CTTAAG
BsaBI 160 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2839 GATTCAAATC
BsgI 2900 CTGCAC GTGCAG(16/14)
3383 CTGCAC
Bsu36I 3673 CCTCAGG CC^T[ACGT]AGG
3706 CCTCAGG
DraII 2877 AGGCCCC [GA]G^G[ACGT]CC[CT]
5265 GGGTCCT
Eco81I 3673 CCTCAGG CC^T[ACGT]AGG
3706 CCTCAGG
EcoO109I 2877 AGGCCCC [GA]G^G[ACGT]CC[CT]
5265 GGGTCCT
EcoRI 583 GAATTC G^AATTC
4310 GAATTC
FbaI 987 TGATCA T^GATCA
2760 TGATCA
MaeII 247 ACGT A^CGT
4724 ACGT
MamI 160 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2839 GATTCAAATC
StuI 3670 AGGCCT AGG^CCT
5274 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AspHI AvaII BanII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI Bsp1286I Bsp1407I BspLU11I
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI EarI EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeIII
HincII HindII HindIII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MfeI MflI MnlI MseI
MslI MspA1I MunI MvaI MwoI
NdeII NlaIII NlaIV NsiI NspI
PflMI PleI PshBI PstI PvuII
RsaI SapI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI