##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AccIII AgeI ApaI AscI
AviII AvrII BcgI BglII BlnI
BsaBI BsaWI BseAI BsiWI Bsp1407I
BspDI BspEI BsrGI BssSI BstAPI
BstBI BstEII BstPI ClaI CpoI
DraIII EcoO65I EcoRV EcoT22I FspI
HpaI MamI MfeI MluI MroI
MunI NheI NotI NruI NsiI
NspV PaeR7I PinAI PmeI PshAI
PvuI RsrII SapI SbfI ScaI
SexAI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 660 GGTACC G^GTACC
AclI 4925 AACGTT AA^CGTT
AhdI 5195 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3601 GTGCAC G^TGCAC
ApaLI 3601 GTGCAC G^TGCAC
AseI 5013 ATTAAT AT^TAAT
AsnI 5013 ATTAAT AT^TAAT
Asp700I 4493 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 660 GGTACC G^GTACC
AspEI 5195 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 5410 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 4889 GGATCC G^GATCC
BbeI 3627 GGCGCC GGCGC^C
BclI 2324 TGATCA T^GATCA
BsaI 1843 GGTCTC GGTCTC(1/5)
BsiEI 48 CGGCCG CG[GA][CT]^CG
BsrBI 4603 CCGCTC CCGCTC(-3/-3)
BsrFI 1941 GCCGGC [GA]^CCGG[CT]
Cfr10I 1941 GCCGGC [GA]^CCGG[CT]
DrdI 2232 GACCCCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 48 CGGCCG C^GGCCG
Eam1105I 5195 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2556 CTCTTC CTCTTC(1/4)
EclXI 48 CGGCCG C^GGCCG
Eco52I 48 CGGCCG C^GGCCG
EcoRI 5488 GAATTC G^AATTC
FbaI 2324 TGATCA T^GATCA
FseI 1940 GGCCGGCC GGCCGG^CC
KasI 3627 GGCGCC G^GCGCC
KpnI 660 GGTACC GGTAC^C
Ksp632I 2556 CTCTTC CTCTTC(1/4)
KspI 224 CCGCGG CCGC^GG
NaeI 1941 GCCGGC GCC^GGC
NarI 3627 GGCGCC GG^CGCC
NcoI 4193 CCATGG C^CATGG
NgoMIV 1941 GCCGGC G^CCGGC
PacI 5014 TTAATTAA TTAAT^TAA
PflFI 5410 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 5013 ATTAAT AT^TAAT
Psp1406I 4925 AACGTT AA^CGTT
SacI 1137 GAGCTC GAGCT^C
SacII 224 CCGCGG CCGC^GG
SalI 486 GTCGAC G^TCGAC
SfiI 49 GGCCGGGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3627 GGCGCC GGC^GCC
SwaI 3966 ATTTAAAT ATTT^AAAT
Tth111I 5410 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 4493 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflII 978 CTTAAG C^TTAAG
3771 CTTAAG
Aor51HI 1666 AGCGCT AGC^GCT
4735 AGCGCT
BanI 660 GGTACC G^G[CT][GA]CC
3627 GGCGCC
BbrPI 2292 CACGTG CAC^GTG
5696 CACGTG
BciVI 931 GGATAC GTATCC(6/5)
646 GTATCC
BfrI 978 CTTAAG C^TTAAG
3771 CTTAAG
BglI 50 GCCGGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1852 GCCCTGGTGGC
BsaAI 2292 CACGTG [CT]AC^GT[GA]
5696 CACGTG
BsmBI 5415 CGTCTC CGTCTC(1/5)
1582 GAGACG
BsmI 3846 GAATGC GAATGC(1/-1)
4629 GAATGC
BspHI 3316 TCATGA T^CATGA
3407 TCATGA
BspMI 313 ACCTGC ACCTGC(4/8)
3758 GCAGGT
BsrDI 1375 CATTGC GCAATG(2/0)
2207 CATTGC
BssHII 253 GCGCGC G^CGCGC
302 GCGCGC
DsaI 224 CCGCGG C^C[GA][CT]GG
4193 CCATGG
Eco47III 1666 AGCGCT AGC^GCT
4735 AGCGCT
EcoNI 1310 CCTCGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3469 CCTTTTAAAGG
HincII 486 GTCGAC GT[CT]^[GA]AC
2671 GTTGAC
HindII 486 GTCGAC GT[CT]^[GA]AC
2671 GTTGAC
HindIII 2172 AAGCTT A^AGCTT
2330 AAGCTT
NdeI 1833 CATATG CA^TATG
3853 CATATG
PmaCI 2292 CACGTG CAC^GTG
5696 CACGTG
PmlI 2292 CACGTG CAC^GTG
5696 CACGTG
PstI 2195 CTGCAG CTGCA^G
4584 CTGCAG
RcaI 3316 TCATGA T^CATGA
3407 TCATGA
SfaNI 2032 GCATC GCATC(5/9)
2454 GCATC
SphI 2189 GCATGC GCATG^C
4543 GCATGC
XcmI 1349 CCAAGCCAACGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1454 CCAGATGTCAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI ApoI AspHI AvaI AvaII
BalI BanII BbsI BbvCI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspLU11I BsrI
BssKI Bst1107I BstF5I BstNI BstUI
BstXI BstYI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII EaeI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfcI SmaI SmlI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmaCI XmaI