##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AccIII AflII Aor51HI AscI
AviII BbeI BbrPI BcgI BciVI
BfrI BlpI Bpu1102I BsaAI BseAI
BsiWI BspDI BspEI BsrBI BsrDI
BssHII BstBI BstEII BstPI CelII
ClaI CpoI DraIII DrdI Eco47III
EcoO65I EcoRV EcoT22I FseI FspI
HaeII KasI MluI MroI NarI
NotI NruI NsiI NspV PacI
PmaCI PmlI PshAI PvuI RsrII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I StuI
SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 5416 GGTACC G^GTACC
AclI 3030 AACGTT AA^CGTT
AcyI 5270 GACGCC G[GA]^CG[CT]C
AgeI 2872 ACCGGT A^CCGGT
Alw44I 1621 GTGCAC G^TGCAC
ApaLI 1621 GTGCAC G^TGCAC
AseI 3726 ATTAAT AT^TAAT
AsnI 3726 ATTAAT AT^TAAT
Asp718I 5416 GGTACC G^GTACC
AvaI 1891 CTCGAG C^[CT]CG[GA]G
AvrII 4236 CCTAGG C^CTAGG
BamHI 3803 GGATCC G^GATCC
BglI 1511 GCCCCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4236 CCTAGG C^CTAGG
BsaBI 300 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 5270 GACGCC G[GA]^CG[CT]C
BsaWI 2872 ACCGGT [AT]^CCGG[AT]
BsiEI 96 CGGCCG CG[GA][CT]^CG
BsoBI 1891 CTCGAG C^[CT]CG[GA]G
Bsp1407I 4638 TGTACA T^GTACA
BspHI 1768 TCATGA T^CATGA
BsrGI 4638 TGTACA T^GTACA
Bst1107I 3843 GTATAC GTA^TAC
BstAPI 2466 GCAGAATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 3843 GTATAC GTA^TAC
EagI 96 CGGCCG C^GGCCG
EclXI 96 CGGCCG C^GGCCG
Eco52I 96 CGGCCG C^GGCCG
EcoNI 2849 CCTGTATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 5270 GACGCC G[GA]^CG[CT]C
HindIII 681 AAGCTT A^AGCTT
KpnI 5416 GGTACC GGTAC^C
MamI 300 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 162 GCCGGC GCC^GGC
NgoMIV 162 GCCGGC G^CCGGC
NheI 2108 GCTAGC G^CTAGC
PaeR7I 1891 CTCGAG C^TCGAG
PinAI 2872 ACCGGT A^CCGGT
PmeI 3166 GTTTAAAC GTTT^AAAC
PshBI 3726 ATTAAT AT^TAAT
Psp1406I 3030 AACGTT AA^CGTT
RcaI 1768 TCATGA T^CATGA
SacI 1468 GAGCTC GAGCT^C
ScaI 4094 AGTACT AGT^ACT
SpeI 156 ACTAGT A^CTAGT
SphI 3976 GCATGC GCATG^C
SspBI 4638 TGTACA T^GTACA
XhoI 1891 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 2365 GTCTAC GT^[AC][GT]AC
3843 GTATAC
AccII 1868 CGCG CG^CG
3341 CGCG
AhdI 2271 GACTTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5198 GACTAGATGTC
AocI 2336 CCTGAGG CC^T[ACGT]AGG
3589 CCTAAGG
ApaI 1872 GGGCCC GGGCC^C
4797 GGGCCC
AspEI 2271 GACTTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5198 GACTAGATGTC
AspI 4166 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5294 GACGGAGTC
BsmBI 5292 GAGACG CGTCTC(1/5)
5463 GAGACG
BspMI 5419 ACCTGC ACCTGC(4/8)
1952 GCAGGT
BsrFI 162 GCCGGC [GA]^CCGG[CT]
2872 ACCGGT
BssSI 77 CTCGTG CACGAG(-5/-1)
5510 CTCGTG
BstUI 1868 CGCG CG^CG
3341 CGCG
Bsu36I 2336 CCTGAGG CC^T[ACGT]AGG
3589 CCTAAGG
Cfr10I 162 GCCGGC [GA]^CCGG[CT]
2872 ACCGGT
Eam1105I 2271 GACTTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5198 GACTAGATGTC
Eco81I 2336 CCTGAGG CC^T[ACGT]AGG
3589 CCTAAGG
FauI 4076 CCCGC CCCGC(4/6)
1869 GCGGG
HgaI 2948 GACGC GACGC(5/10)
5270 GACGC
HpaI 368 GTTAAC GTT^AAC
4586 GTTAAC
KspI 1867 CCGCGG CCGC^GG
3340 CCGCGG
MvnI 1868 CGCG CG^CG
3341 CGCG
NdeI 3817 CATATG CA^TATG
5684 CATATG
PflFI 4166 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5294 GACGGAGTC
PflMI 2117 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3923 CCACCTCCTGG
PpuMI 1494 GGGACCC [GA]G^G[AT]CC[CT]
2332 AGGTCCT
SacII 1867 CCGCGG CCGC^GG
3340 CCGCGG
SapI 426 GAAGAGC GCTCTTC(1/4)
1062 GAAGAGC
Tth111I 4166 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5294 GACGGAGTC
Van91I 2117 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3923 CCACCTCCTGG
XbaI 2057 TCTAGA T^CTAGA
2519 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BclI BcnI BfaI
BglII BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspLU11I BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRI EcoRII
EcoT14I FbaI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MunI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI