##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
AocI Aor51HI ApaI AscI AspEI
AspI AvrII BbeI BlnI BsaBI
BsaI BseAI BsiWI BspDI BspEI
BssHII BssSI Bst1107I BstEII BstPI
BstZ17I Bsu36I ClaI Eam1105I Eco47III
Eco81I EcoNI EcoO65I FseI KasI
KspI MamI MfeI MluI MroI
MunI NaeI NarI NgoMIV NheI
NotI NruI PaeR7I PflFI PinAI
PmeI PshAI Psp1406I PvuI SacII
SbfI SfiI SfoI SgrAI SnaBI
Sse8387I Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 4002 GGTACC G^GTACC
Alw44I 1677 GTGCAC G^TGCAC
ApaLI 1677 GTGCAC G^TGCAC
Asp718I 4002 GGTACC G^GTACC
AvaI 347 CCCGGG C^[CT]CG[GA]G
BamHI 4074 GGATCC G^GATCC
BbrPI 1642 CACGTG CAC^GTG
BciVI 5566 GTATCC GTATCC(6/5)
BclI 2366 TGATCA T^GATCA
BglI 2725 GCCCAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 4860 TCCGGT [AT]^CCGG[AT]
BseRI 268 GAGGAG GAGGAG(10/8)
BsmBI 574 GAGACG CGTCTC(1/5)
BsoBI 347 CCCGGG C^[CT]CG[GA]G
BsrBI 1866 CCGCTC CCGCTC(-3/-3)
BstBI 2300 TTCGAA TT^CGAA
CpoI 1742 CGGACCG CG^G[AT]CCG
EcoRV 5590 GATATC GAT^ATC
EcoT22I 1822 ATGCAT ATGCA^T
FbaI 2366 TGATCA T^GATCA
HaeII 2401 AGCGCC [GA]GCGC^[CT]
HindIII 3952 AAGCTT A^AGCTT
HpaI 2022 GTTAAC GTT^AAC
KpnI 4002 GGTACC GGTAC^C
NdeI 4732 CATATG CA^TATG
NsiI 1822 ATGCAT ATGCA^T
NspV 2300 TTCGAA TT^CGAA
PacI 4521 TTAATTAA TTAAT^TAA
PmaCI 1642 CACGTG CAC^GTG
PmlI 1642 CACGTG CAC^GTG
RsrII 1742 CGGACCG CG^G[AT]CCG
SalI 947 GTCGAC G^TCGAC
SapI 1291 GCTCTTC GCTCTTC(1/4)
ScaI 4697 AGTACT AGT^ACT
SfuI 2300 TTCGAA TT^CGAA
SmaI 347 CCCGGG CCC^GGG
SpeI 4889 ACTAGT A^CTAGT
SwaI 5666 ATTTAAAT ATTT^AAAT
XmaCI 347 CCCGGG C^CCGGG
XmaI 347 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 947 GTCGAC GT^[AC][GT]AC
5404 GTAGAC
AccII 282 CGCG CG^CG
1957 CGCG
AcyI 959 GGCGTC G[GA]^CG[CT]C
2538 GGCGTC
AflII 5160 CTTAAG C^TTAAG
5585 CTTAAG
AseI 4524 ATTAAT AT^TAAT
5742 ATTAAT
AsnI 4524 ATTAAT AT^TAAT
5742 ATTAAT
AviII 1250 TGCGCA TGC^GCA
1646 TGCGCA
BcgI 2426 CGACGGACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3060 CGAGCCACCTGC
BfrI 5160 CTTAAG C^TTAAG
5585 CTTAAG
BglII 1667 AGATCT A^GATCT
3400 AGATCT
BsaHI 959 GGCGTC G[GA]^CG[CT]C
2538 GGCGTC
Bsp1407I 4125 TGTACA T^GTACA
5686 TGTACA
BsrFI 332 ACCGGC [GA]^CCGG[CT]
336 GCCGGT
BsrGI 4125 TGTACA T^GTACA
5686 TGTACA
BstUI 282 CGCG CG^CG
1957 CGCG
BstXI 2694 CCAGGTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3549 CCAAGTTCATGG
Cfr10I 332 ACCGGC [GA]^CCGG[CT]
336 GCCGGT
DrdI 2165 GACCCGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2451 GACAGAACTGTC
EagI 334 CGGCCG C^GGCCG
952 CGGCCG
EclXI 334 CGGCCG C^GGCCG
952 CGGCCG
Eco52I 334 CGGCCG C^GGCCG
952 CGGCCG
FspI 1250 TGCGCA TGC^GCA
1646 TGCGCA
Hin1I 959 GGCGTC G[GA]^CG[CT]C
2538 GGCGTC
MvnI 282 CGCG CG^CG
1957 CGCG
NcoI 1395 CCATGG C^CATGG
3583 CCATGG
PpuMI 5092 AGGACCT [GA]G^G[AT]CC[CT]
5292 AGGACCT
PshBI 4524 ATTAAT AT^TAAT
5742 ATTAAT
SacI 2137 GAGCTC GAGCT^C
3259 GAGCTC
SexAI 56 ACCAGGT A^CC[AT]GGT
632 ACCTGGT
SphI 285 GCATGC GCATG^C
1961 GCATGC
SspBI 4125 TGTACA T^GTACA
5686 TGTACA
XbaI 4266 TCTAGA T^CTAGA
4865 TCTAGA
XcmI 250 CCACACGACTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
674 CCATAAAAAATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaJI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspHI BspLU11I BspMI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EarI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI