##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflIII AgeI Alw44I ApaLI AscI
Asp700I Asp718I BbeI BbrPI BcgI
BciVI BsaAI BsaHI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspLU11I
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI EagI EclXI
Eco52I EcoRI EcoRV Hin1I HindIII
KasI KpnI KspI MfeI MluI
MroI MunI NarI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SwaI XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AflII 4717 CTTAAG C^TTAAG
AhdI 3093 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2111 AGCGCT AGC^GCT
AseI 910 ATTAAT AT^TAAT
AsnI 910 ATTAAT AT^TAAT
AspEI 3093 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1312 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 581 TGCGCA TGC^GCA
AvrII 3516 CCTAGG C^CTAGG
BamHI 1357 GGATCC G^GATCC
BanI 4483 GGTGCC G^G[CT][GA]CC
BfrI 4717 CTTAAG C^TTAAG
BlnI 3516 CCTAGG C^CTAGG
BsaBI 835 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 593 ACCGGA [AT]^CCGG[AT]
BssSI 4844 CACGAG CACGAG(-5/-1)
BstEII 948 GGTGACC G^GT[ACGT]ACC
BstPI 948 GGTGACC G^GT[ACGT]ACC
Eam1105I 3093 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2111 AGCGCT AGC^GCT
EcoO65I 948 GGTGACC G^GT[ACGT]ACC
EcoT22I 2911 ATGCAT ATGCA^T
FseI 666 GGCCGGCC GGCCGG^CC
FspI 581 TGCGCA TGC^GCA
HaeII 2111 AGCGCT [GA]GCGC^[CT]
MamI 835 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI 2911 ATGCAT ATGCA^T
NspI 903 GCATGT [GA]CATG^[CT]
PflFI 1312 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 4405 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 910 ATTAAT AT^TAAT
ScaI 1736 AGTACT AGT^ACT
SpeI 3885 ACTAGT A^CTAGT
SspI 5056 AATATT AAT^ATT
Tth111I 1312 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 3267 GTAGAC GT^[AC][GT]AC
5036 GTCTAC
AccII 187 CGCG CG^CG
2757 CGCG
BbsI 4146 GTCTTC GAAGAC(2/6)
4787 GTCTTC
BbvCI 4 GCTGAGG CCTCAGC(-5/-2)
3061 GCTGAGG
BglII 1694 AGATCT A^GATCT
4754 AGATCT
BpuAI 4146 GTCTTC GAAGAC(2/6)
4787 GTCTTC
BsaI 590 GAGACC GGTCTC(1/5)
3963 GAGACC
BsmI 627 GAATGC GAATGC(1/-1)
2909 GAATGC
Bsp1407I 1752 TGTACA T^GTACA
3280 TGTACA
BspHI 636 TCATGA T^CATGA
3166 TCATGA
BsrGI 1752 TGTACA T^GTACA
3280 TGTACA
BstUI 187 CGCG CG^CG
2757 CGCG
DraIII 1420 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
4243 CACAAGGTG
DsaI 353 CCATGG C^C[GA][CT]GG
1931 CCATGG
HgaI 3486 GACGC GACGC(5/10)
3163 GCGTC
HincII 3620 GTTAAC GT[CT]^[GA]AC
3803 GTTAAC
HindII 3620 GTTAAC GT[CT]^[GA]AC
3803 GTTAAC
HpaI 3620 GTTAAC GTT^AAC
3803 GTTAAC
MvnI 187 CGCG CG^CG
2757 CGCG
NcoI 353 CCATGG C^CATGG
1931 CCATGG
NheI 2114 GCTAGC G^CTAGC
4066 GCTAGC
PflMI 1419 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3230 CCAACAGCTGG
PstI 647 CTGCAG CTGCA^G
1591 CTGCAG
RcaI 636 TCATGA T^CATGA
3166 TCATGA
SmlI 2607 CTTGAG C^T[CT][GA]AG
4717 CTTAAG
SspBI 1752 TGTACA T^GTACA
3280 TGTACA
Van91I 1419 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3230 CCAACAGCTGG
XcmI 3113 CCAGTTCTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4200 CCACATCTGTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI AspHI
AvaI AvaII BalI BanII BbvI
BclI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NaeI NciI
NdeI NdeII NgoMIV NlaIII NlaIV
PleI PpuMI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII