##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
Acc65I AccIII AclI AgeI Aor51HI
AscI Asp718I BamHI BsaWI BseAI
BspDI BspEI Bst1107I BstBI BstZ17I
ClaI CpoI Eco47III EcoRI EcoRV
FseI HpaI KpnI MfeI MluI
MroI MunI NheI NotI NruI
NspV PacI PaeR7I PinAI PmeI
Psp1406I RsrII SalI SfiI SfuI
SgrAI SnaBI SpeI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 3505 GACGTC GACGT^C
AccI 2239 GTCTAC GT^[AC][GT]AC
AhdI 4846 GACTGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 3194 ATTAAT AT^TAAT
AsnI 3194 ATTAAT AT^TAAT
Asp700I 285 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4846 GACTGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2329 TGCGCA TGC^GCA
BcgI 1685 CGACGCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 3970 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2972 GGTCTC GGTCTC(1/5)
BsiWI 80 CGTACG C^GTACG
BsmI 3746 GCATTC GAATGC(1/-1)
BspHI 815 TCATGA T^CATGA
BsrBI 1098 GAGCGG CCGCTC(-3/-3)
BssHII 965 GCGCGC G^CGCGC
BssSI 1614 CACGAG CACGAG(-5/-1)
DraI 5584 TTTAAA TTT^AAA
DrdI 28 GACTGTCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 332 CGGCCG C^GGCCG
Eam1105I 4846 GACTGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 332 CGGCCG C^GGCCG
Eco52I 332 CGGCCG C^GGCCG
FspI 2329 TGCGCA TGC^GCA
HincII 5155 GTTGAC GT[CT]^[GA]AC
HindII 5155 GTTGAC GT[CT]^[GA]AC
MamI 3970 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 43 CATATG CA^TATG
PshAI 355 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3194 ATTAAT AT^TAAT
PvuI 470 CGATCG CGAT^CG
RcaI 815 TCATGA T^CATGA
SbfI 4507 CCTGCAGG CCTGCA^GG
SexAI 1532 ACCAGGT A^CC[AT]GGT
SphI 2326 GCATGC GCATG^C
Sse8387I 4507 CCTGCAGG CCTGCA^GG
SspI 5685 AATATT AAT^ATT
StuI 4504 AGGCCT AGG^CCT
XbaI 2809 TCTAGA T^CTAGA
XmnI 285 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AflIII 4866 ACATGT A^C[GA][CT]GT
5716 ACATGT
AvrII 3160 CCTAGG C^CTAGG
3717 CCTAGG
BalI 197 TGGCCA TGG^CCA
2156 TGGCCA
BciVI 3248 GGATAC GTATCC(6/5)
4659 GTATCC
BclI 2479 TGATCA T^GATCA
4478 TGATCA
BglII 2229 AGATCT A^GATCT
4650 AGATCT
BlnI 3160 CCTAGG C^CTAGG
3717 CCTAGG
BsmBI 4143 GAGACG CGTCTC(1/5)
4935 GAGACG
Bsp1407I 1856 TGTACA T^GTACA
3124 TGTACA
BspLU11I 4866 ACATGT A^CATGT
5716 ACATGT
BsrDI 915 CATTGC GCAATG(2/0)
3151 CATTGC
BsrGI 1856 TGTACA T^GTACA
3124 TGTACA
BstXI 1706 CCATCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3353 CCACCCGGCTGG
EarI 4806 CTCTTC CTCTTC(1/4)
285 GAAGAG
EcoT22I 458 ATGCAT ATGCA^T
4685 ATGCAT
FbaI 2479 TGATCA T^GATCA
4478 TGATCA
Ksp632I 4806 CTCTTC CTCTTC(1/4)
285 GAAGAG
MluNI 197 TGGCCA TGG^CCA
2156 TGGCCA
MscI 197 TGGCCA TGG^CCA
2156 TGGCCA
NaeI 334 GCCGGC GCC^GGC
560 GCCGGC
NgoMIV 334 GCCGGC G^CCGGC
560 GCCGGC
NsiI 458 ATGCAT ATGCA^T
4685 ATGCAT
PflMI 2118 CCAGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3333 CCACGGTCTGG
SapI 285 GAAGAGC GCTCTTC(1/4)
4805 GCTCTTC
ScaI 692 AGTACT AGT^ACT
3987 AGTACT
SmaI 767 CCCGGG CCC^GGG
2131 CCCGGG
SspBI 1856 TGTACA T^GTACA
3124 TGTACA
TaqI 38 TCGA T^CGA
4927 TCGA
TthHB8I 38 TCGA T^CGA
4927 TCGA
Van91I 2118 CCAGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3333 CCACGGTCTGG
XmaCI 767 CCCGGG C^CCGGG
2131 CCCGGG
XmaI 767 CCCGGG C^CCGGG
2131 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflII AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI ApoI AspHI
AspI AvaI AvaII BanI BanII
BbeI BbrPI BbsI BbvCI BbvI
BcnI BfaI BfrI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI KasI KspI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV NspI PflFI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI SacI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I XcmI
XhoII