##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AlwNI AocI Aor51HI ApaI
AscI Asp718I AspI BamHI BbeI
BbrPI BbvCI BglI BglII BlpI
Bpu1102I BsaBI BsaHI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspHI
BsrBI BssHII BssSI BstYI Bsu36I
CelII ClaI CpoI DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
FseI Hin1I KasI KpnI KspI
MamI MfeI MflI MluI MroI
MunI NarI NotI NruI PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI Sse8387I Tth111I XcmI XhoII
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccII 1304 CGCG CG^CG
AhdI 861 GACAGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3089 GTGCAC G^TGCAC
ApaLI 3089 GTGCAC G^TGCAC
AspEI 861 GACAGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1337 CTCGAG C^[CT]CG[GA]G
AviII 4066 TGCGCA TGC^GCA
AvrII 492 CCTAGG C^CTAGG
BbsI 880 GAAGAC GAAGAC(2/6)
BcgI 1239 CGAAAATTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1763 CCCGG CC^[GC]GG
BlnI 492 CCTAGG C^CTAGG
BpuAI 880 GAAGAC GAAGAC(2/6)
BsaAI 1436 TACGTG [CT]AC^GT[GA]
BsaWI 2653 TCCGGT [AT]^CCGG[AT]
BsgI 3971 GTGCAG GTGCAG(16/14)
BsoBI 1337 CTCGAG C^[CT]CG[GA]G
BspMI 1616 ACCTGC ACCTGC(4/8)
Bst1107I 2657 GTATAC GTA^TAC
BstAPI 1723 GCAGACTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3025 TTCGAA TT^CGAA
BstEII 5280 GGTAACC G^GT[ACGT]ACC
BstPI 5280 GGTAACC G^GT[ACGT]ACC
BstUI 1304 CGCG CG^CG
BstXI 1060 CCAACCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2657 GTATAC GTA^TAC
DraIII 2368 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 861 GACAGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 5280 GGTAACC G^GT[ACGT]ACC
EcoRI 4536 GAATTC G^AATTC
EcoRV 4525 GATATC GAT^ATC
FspI 4066 TGCGCA TGC^GCA
HaeII 1386 AGCGCC [GA]GCGC^[CT]
HgaI 1646 GCGTC GACGC(5/10)
HindIII 3696 AAGCTT A^AGCTT
HpaI 2872 GTTAAC GTT^AAC
MspA1I 1948 CAGCTG C[AC]G^C[GT]G
MvnI 1304 CGCG CG^CG
NaeI 1754 GCCGGC GCC^GGC
NciI 1763 CCCGG CC^[GC]GG
NcoI 1197 CCATGG C^CATGG
NdeI 4235 CATATG CA^TATG
NgoMIV 1754 GCCGGC G^CCGGC
NspV 3025 TTCGAA TT^CGAA
PacI 4218 TTAATTAA TTAAT^TAA
PaeR7I 1337 CTCGAG C^TCGAG
PpuMI 1824 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 3636 CTGCAG CTGCA^G
PvuII 1948 CAGCTG CAG^CTG
SfcI 3636 CTGCAG C^T[GA][CT]AG
SfuI 3025 TTCGAA TT^CGAA
SphI 3095 GCATGC GCATG^C
StuI 3279 AGGCCT AGG^CCT
SwaI 2947 ATTTAAAT ATTT^AAAT
XhoI 1337 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflII 3488 CTTAAG C^TTAAG
4588 CTTAAG
AlwI 5246 GATCC GGATC(4/5)
1007 GGATC
AseI 2671 ATTAAT AT^TAAT
4099 ATTAAT
AsnI 2671 ATTAAT AT^TAAT
4099 ATTAAT
Asp700I 708 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4536 GAATTCCTTC
BanI 1468 GGCACC G^G[CT][GA]CC
4890 GGTGCC
BanII 2507 GGGCTC G[GA]GC[CT]^C
3641 GAGCCC
BclI 818 TGATCA T^GATCA
3835 TGATCA
BfrI 3488 CTTAAG C^TTAAG
4588 CTTAAG
BsaI 2135 GAGACC GGTCTC(1/5)
4249 GAGACC
BseRI 931 GAGGAG GAGGAG(10/8)
4011 GAGGAG
BsrFI 110 GCCGGT [GA]^CCGG[CT]
1754 GCCGGC
Cfr10I 110 GCCGGT [GA]^CCGG[CT]
1754 GCCGGC
DsaI 1197 CCATGG C^C[GA][CT]GG
1784 CCGTGG
FbaI 818 TGATCA T^GATCA
3835 TGATCA
NheI 1566 GCTAGC G^CTAGC
1953 GCTAGC
PshBI 2671 ATTAAT AT^TAAT
4099 ATTAAT
SspI 2968 AATATT AAT^ATT
3914 AATATT
XbaI 1107 TCTAGA T^CTAGA
1701 TCTAGA
XmnI 708 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4536 GAATTCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI ApoI AspHI AvaII BalI
BbvI BciVI BfaI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BspLU11I
BsrDI BsrGI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EaeI EarI
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV NsiI
NspI PflMI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI SpeI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I