##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII Aor51HI ApaI AscI AvaI
AviII AvrII BamHI BbeI BcgI
BciVI BfrI BglI BlnI BsaBI
BsaHI BseAI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BsrBI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I CfoI ClaI CpoI
DraIII DrdI DsaI EagI EclXI
Eco47III Eco52I EcoO65I EcoT22I FauI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HpaI KasI KspI
MamI MluI MroI MvnI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspV PaeR7I PshAI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 2364 GGTACC G^GTACC
AgeI 797 ACCGGT A^CCGGT
AhdI 32 GACCAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2471 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 276 CCTGAGG CC^T[ACGT]AGG
Asp718I 2364 GGTACC G^GTACC
AspEI 32 GACCAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3495 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 2364 GGTACC G^G[CT][GA]CC
BbrPI 3939 CACGTG CAC^GTG
BbvCI 3712 CCTCAGC CCTCAGC(-5/-2)
BclI 2078 TGATCA T^GATCA
BcnI 1583 CCCGG CC^[GC]GG
BglII 239 AGATCT A^GATCT
BsaAI 3939 CACGTG [CT]AC^GT[GA]
BsaI 30 GAGACC GGTCTC(1/5)
BsaWI 797 ACCGGT [AT]^CCGG[AT]
BspHI 5088 TCATGA T^CATGA
BsrFI 797 ACCGGT [GA]^CCGG[CT]
Bsu36I 276 CCTGAGG CC^T[ACGT]AGG
Cfr10I 797 ACCGGT [GA]^CCGG[CT]
Eam1105I 32 GACCAATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 276 CCTGAGG CC^T[ACGT]AGG
EcoRV 4723 GATATC GAT^ATC
FbaI 2078 TGATCA T^GATCA
HincII 1560 GTCAAC GT[CT]^[GA]AC
HindII 1560 GTCAAC GT[CT]^[GA]AC
KpnI 2364 GGTACC GGTAC^C
MfeI 35 CAATTG C^AATTG
MunI 35 CAATTG C^AATTG
NciI 1583 CCCGG CC^[GC]GG
PacI 4429 TTAATTAA TTAAT^TAA
PflFI 3495 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 797 ACCGGT A^CCGGT
PmaCI 3939 CACGTG CAC^GTG
PmeI 2592 GTTTAAAC GTTT^AAAC
PmlI 3939 CACGTG CAC^GTG
RcaI 5088 TCATGA T^CATGA
SapI 1980 GAAGAGC GCTCTTC(1/4)
ScaI 1119 AGTACT AGT^ACT
SpeI 3462 ACTAGT A^CTAGT
StuI 531 AGGCCT AGG^CCT
Tth111I 3495 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 212 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Alw44I 289 GTGCAC G^TGCAC
2503 GTGCAC
AlwI 1029 GATCC GGATC(4/5)
48 GGATC
ApaLI 289 GTGCAC G^TGCAC
2503 GTGCAC
BlpI 4940 GCTCAGC GC^T[ACGT]AGC
4957 GCTCAGC
Bpu1102I 4940 GCTCAGC GC^T[ACGT]AGC
4957 GCTCAGC
BsmFI 4968 GGGAC GGGAC(10/14)
5226 GTCCC
BsmI 5237 GAATGC GAATGC(1/-1)
4347 GCATTC
BspMI 5299 ACCTGC ACCTGC(4/8)
3622 GCAGGT
BstAPI 1387 GCACTGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3521 GCAAAGGATGC
BstXI 754 CCAAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3578 CCAGCAAGTTGG
BstYI 239 AGATCT [GA]^GATC[CT]
1028 AGATCC
CelII 4940 GCTCAGC GC^T[ACGT]AGC
4957 GCTCAGC
EcoNI 130 CCTCTTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2524 CCTCTCTTAGG
EcoRI 3259 GAATTC G^AATTC
5507 GAATTC
EcoT14I 2301 CCTTGG C^C[AT][AT]GG
4628 CCAAGG
HapII 798 CCGG C^CGG
1584 CCGG
HpaII 798 CCGG C^CGG
1584 CCGG
MflI 239 AGATCT [GA]^GATC[CT]
1028 AGATCC
MspA1I 1018 CAGCTG C[AC]G^C[GT]G
4960 CAGCTG
MspI 798 CCGG C^CGG
1584 CCGG
NdeI 2181 CATATG CA^TATG
3196 CATATG
PflMI 2446 CCACAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2897 CCAGTGTTTGG
PvuII 1018 CAGCTG CAG^CTG
4960 CAGCTG
SacI 1126 GAGCTC GAGCT^C
3311 GAGCTC
SmlI 1812 CTTGAG C^T[CT][GA]AG
2085 CTTGAG
SspI 2836 AATATT AAT^ATT
4317 AATATT
StyI 2301 CCTTGG C^C[AT][AT]GG
4628 CCAAGG
Van91I 2446 CCACAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2897 CCAGTGTTTGG
XhoII 239 AGATCT [GA]^GATC[CT]
1028 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI ApoI AseI AsnI Asp700I
AspHI AvaII BalI BanII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI Bsp1286I Bsp1407I BspLU11I
BsrDI BsrGI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraI DraII EaeI EarI EcoO109I
EcoRII Fnu4HI FokI HaeIII HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PpuMI PshBI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspBI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XmnI