##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AgeI AhdI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AvaI AviII AvrII BbeI BbrPI
BcgI BciVI BcnI BfrI BglI
BlnI BsaBI BsaHI BsaI BsiEI
BsiWI BsmBI BsoBI BspDI BspMI
BsrDI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstXI BstZ17I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV FseI FspI
HaeII HgaI Hin1I HpaI KasI
KpnI KspI MamI MfeI MluI
MslI MunI MvnI NarI NciI
NdeI NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SpeI
SphI Sse8387I SspI StuI SwaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 19 enzymes) #
###################################
Alw44I 234 GTGCAC G^TGCAC
ApaLI 234 GTGCAC G^TGCAC
BamHI 693 GGATCC G^GATCC
BclI 716 TGATCA T^GATCA
BglII 498 AGATCT A^GATCT
BsmAI 3212 GTCTC GTCTC(1/5)
BsmI 22 GCATTC GAATGC(1/-1)
Bsp1407I 3152 TGTACA T^GTACA
BsrGI 3152 TGTACA T^GTACA
DraI 344 TTTAAA TTT^AAA
DraIII 252 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 300 GAATTC G^AATTC
FbaI 716 TGATCA T^GATCA
NcoI 3038 CCATGG C^CATGG
PstI 3225 CTGCAG CTGCA^G
SalI 3102 GTCGAC G^TCGAC
SapI 272 GAAGAGC GCTCTTC(1/4)
ScaI 1313 AGTACT AGT^ACT
SspBI 3152 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 8 enzymes) #
###################################
AcsI 300 GAATTC [GA]^AATT[CT]
972 AAATTT
ApoI 300 GAATTC [GA]^AATT[CT]
972 AAATTT
BsgI 1341 GTGCAG GTGCAG(16/14)
2940 GTGCAG
BstEII 66 GGTGACC G^GT[ACGT]ACC
590 GGTTACC
BstPI 66 GGTGACC G^GT[ACGT]ACC
590 GGTTACC
EcoO65I 66 GGTGACC G^GT[ACGT]ACC
590 GGTTACC
HphI 66 GGTGA GGTGA(8/7)
687 GGTGA
SmlI 378 CTCAAG C^T[CT][GA]AG
2530 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccI AccIII AciI AfaI AflIII
AluI AlwI AlwNI AocI Asp700I
AspHI AspI AvaII BalI BanI
BanII BbsI BbvCI BbvI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaJI BsaWI BseAI BseRI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BspEI BspHI BspLU11I BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HindIII HinfI HpaII
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MroI MscI MseI MspA1I MspI
MvaI MwoI NaeI NdeII NgoMIV
NlaIII NlaIV NsiI NspI PflFI
PflMI PleI PpuMI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SnaBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XbaI
XhoII XmnI