##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
Acc65I AclI AcsI AflII AflIII
AgeI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I BbrPI BcgI BciVI BclI
BfrI BglII BsaAI BsaBI BsiWI
Bsp1407I BspDI BspHI BspLU11I BsrGI
BssHII Bst1107I BstBI BstZ17I Bsu36I
ClaI DraI DraIII Eco47III Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
HindIII HpaI KpnI MamI MluI
NdeI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI SapI
SbfI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 517 GTCGAC GT^[AC][GT]AC
AccIII 128 TCCGGA T^CCGGA
AhdI 974 GACGCATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 974 GACGCATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 708 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 152 CCTAGG C^CTAGG
BamHI 2387 GGATCC G^GATCC
BbvCI 1608 CCTCAGC CCTCAGC(-5/-2)
BlnI 152 CCTAGG C^CTAGG
BlpI 996 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 996 GCTGAGC GC^T[ACGT]AGC
BsaWI 128 TCCGGA [AT]^CCGG[AT]
BseAI 128 TCCGGA T^CCGGA
BsgI 1631 CTGCAC GTGCAG(16/14)
BsmBI 2948 CGTCTC CGTCTC(1/5)
BspEI 128 TCCGGA T^CCGGA
BstAPI 1092 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1186 GGTAACC G^GT[ACGT]ACC
BstPI 1186 GGTAACC G^GT[ACGT]ACC
CelII 996 GCTGAGC GC^T[ACGT]AGC
CpoI 2543 CGGACCG CG^G[AT]CCG
DrdI 2940 GACAGTTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 974 GACGCATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1186 GGTAACC G^GT[ACGT]ACC
KspI 394 CCGCGG CCGC^GG
MfeI 1203 CAATTG C^AATTG
MroI 128 TCCGGA T^CCGGA
MseI 183 TTAA T^TAA
MunI 1203 CAATTG C^AATTG
NaeI 401 GCCGGC GCC^GGC
NgoMIV 401 GCCGGC G^CCGGC
NheI 489 GCTAGC G^CTAGC
PflFI 708 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1470 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 2543 CGGACCG CG^G[AT]CCG
SacI 2284 GAGCTC GAGCT^C
SacII 394 CCGCGG CCGC^GG
SalI 517 GTCGAC G^TCGAC
ScaI 533 AGTACT AGT^ACT
StuI 1886 AGGCCT AGG^CCT
Tru9I 183 TTAA T^TAA
Tsp509I 1204 AATT ^AATT
Tth111I 708 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AatII 284 GACGTC GACGT^C
2520 GACGTC
AviII 2276 TGCGCA TGC^GCA
2863 TGCGCA
BalI 1940 TGGCCA TGG^CCA
2093 TGGCCA
BbeI 388 GGCGCC GGCGC^C
2206 GGCGCC
BbsI 70 GAAGAC GAAGAC(2/6)
2107 GTCTTC
BpuAI 70 GAAGAC GAAGAC(2/6)
2107 GTCTTC
BsiEI 2174 CGGCCG CG[GA][CT]^CG
3014 CGGCCG
BsmI 108 GAATGC GAATGC(1/-1)
308 GCATTC
BsrDI 1542 CATTGC GCAATG(2/0)
2183 GCAATG
BssSI 2149 CACGAG CACGAG(-5/-1)
2966 CTCGTG
EagI 2174 CGGCCG C^GGCCG
3014 CGGCCG
EclXI 2174 CGGCCG C^GGCCG
3014 CGGCCG
Eco52I 2174 CGGCCG C^GGCCG
3014 CGGCCG
EcoNI 438 CCTCGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
807 CCTTGGGGAGG
FspI 2276 TGCGCA TGC^GCA
2863 TGCGCA
HgaI 520 GACGC GACGC(5/10)
974 GACGC
KasI 388 GGCGCC G^GCGCC
2206 GGCGCC
MaeII 285 ACGT A^CGT
2521 ACGT
MluNI 1940 TGGCCA TGG^CCA
2093 TGGCCA
MscI 1940 TGGCCA TGG^CCA
2093 TGGCCA
NarI 388 GGCGCC GG^CGCC
2206 GGCGCC
PpuMI 568 GGGACCC [GA]G^G[AT]CC[CT]
1406 GGGACCC
SexAI 1904 ACCTGGT A^CC[AT]GGT
2774 ACCTGGT
SfoI 388 GGCGCC GGC^GCC
2206 GGCGCC
SphI 2747 GCATGC GCATG^C
2846 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PaeR7I PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I Van91I XcmI XhoI
XhoII XmaCI XmaI