##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AflII AflIII AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbeI BbrPI BbsI BcgI
BciVI BclI BfrI BglII BlnI
BpuAI BsaBI BsiEI BsiWI BsmI
Bsp1407I BspDI BspLU11I BspMI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I CfoI ClaI CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI FbaI FseI FspI
HaeII HhaI HinP1I HincII HindII
HpaI KasI KpnI KspI MamI
MfeI MflI MluI MluNI MscI
MunI MvnI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XhoI XhoII
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AgeI 1018 ACCGGT A^CCGGT
ApaI 1074 GGGCCC GGGCC^C
AspHI 609 GAGCTC G[AT]GC[AT]^C
AvaI 1079 CCCGGG C^[CT]CG[GA]G
BlpI 1790 GCTAAGC GC^T[ACGT]AGC
BpmI 433 CTGGAG CTGGAG(16/14)
Bpu1102I 1790 GCTAAGC GC^T[ACGT]AGC
BsaAI 1571 TACGTG [CT]AC^GT[GA]
BsiHKAI 609 GAGCTC G[AT]GC[AT]^C
BsmBI 1887 CGTCTC CGTCTC(1/5)
BsoBI 1079 CCCGGG C^[CT]CG[GA]G
BspHI 791 TCATGA T^CATGA
BsrBI 1025 CCGCTC CCGCTC(-3/-3)
BsrFI 1018 ACCGGT [GA]^CCGG[CT]
CelII 1790 GCTAAGC GC^T[ACGT]AGC
Cfr10I 1018 ACCGGT [GA]^CCGG[CT]
DrdI 959 GACTCGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 103 CGGCCA [CT]^GGCC[GA]
EcoNI 1392 CCTGATCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1379 ATGCAT ATGCA^T
HindIII 1793 AAGCTT A^AGCTT
MaeII 1572 ACGT A^CGT
MspA1I 879 CAGCTG C[AC]G^C[GT]G
NdeI 1376 CATATG CA^TATG
NsiI 1379 ATGCAT ATGCA^T
PinAI 1018 ACCGGT A^CCGGT
PvuII 879 CAGCTG CAG^CTG
RcaI 791 TCATGA T^CATGA
SacI 609 GAGCTC GAGCT^C
SapI 606 GAAGAGC GCTCTTC(1/4)
ScaI 916 AGTACT AGT^ACT
SmaI 1079 CCCGGG CCC^GGG
SmlI 849 CTCAAG C^T[CT][GA]AG
TaqI 247 TCGA T^CGA
Tsp45I 141 GTCAC ^GT[GC]AC
TthHB8I 247 TCGA T^CGA
XbaI 1767 TCTAGA T^CTAGA
XmaCI 1079 CCCGGG C^CCGGG
XmaI 1079 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccIII 572 TCCGGA T^CCGGA
864 TCCGGA
AcyI 1 GGCGTC G[GA]^CG[CT]C
92 GACGCC
AlwNI 231 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
1286 CAGCTCCTG
Asp700I 261 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1060 GAACTCTTTC
BbvCI 1411 GCTGAGG CCTCAGC(-5/-2)
1522 GCTGAGG
BglI 30 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1076 GCCCCCGGGGC
BsaHI 1 GGCGTC G[GA]^CG[CT]C
92 GACGCC
BseAI 572 TCCGGA T^CCGGA
864 TCCGGA
BspEI 572 TCCGGA T^CCGGA
864 TCCGGA
BstAPI 47 GCAGGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1213 GCAGATCATGC
DraIII 114 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
198 CACGATGTG
DsaI 34 CCATGG C^C[GA][CT]GG
1090 CCATGG
EcoRV 337 GATATC GAT^ATC
741 GATATC
Hin1I 1 GGCGTC G[GA]^CG[CT]C
92 GACGCC
MaeIII 141 GTCAC ^GT[ACGT]AC
814 GTAAC
MroI 572 TCCGGA T^CCGGA
864 TCCGGA
NcoI 34 CCATGG C^CATGG
1090 CCATGG
NspI 253 ACATGC [GA]CATG^[CT]
659 ACATGC
PpuMI 1132 GGGACCC [GA]G^G[AT]CC[CT]
1319 GGGACCC
PstI 45 CTGCAG CTGCA^G
387 CTGCAG
StuI 554 AGGCCT AGG^CCT
907 AGGCCT
TfiI 1277 GATTC G^A[AT]TC
1926 GAATC
XmnI 261 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1060 GAACTCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI AvaII BanI BanII
BbvI BcnI BfaI BmyI BsaI
BsaJI BsaWI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Bsu36I Cac8I
Cfr13I DdeI DpnII DraII EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI Ksp632I
MaeI MboI MboII MnlI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI Tru9I TseI Tsp509I
TspRI Van91I XcmI