##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Aor51HI ApaI AscI Asp718I AviII
BbeI BbrPI BclI BsaAI BsaBI
BsaWI BseAI BsiWI BspDI BspEI
BsrBI BssHII BstAPI BstBI BstEII
BstPI ClaI CpoI DrdI Eco47III
EcoO65I EcoT22I FbaI FseI FspI
HaeII HpaI KasI KpnI KspI
MamI MluI MroI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacII SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AcyI 609 GACGCC G[GA]^CG[CT]C
AhdI 1987 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1975 GTGCAC G^TGCAC
ApaLI 1975 GTGCAC G^TGCAC
AseI 5712 ATTAAT AT^TAAT
AsnI 5712 ATTAAT AT^TAAT
AspEI 1987 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1857 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 1188 GGATAC GTATCC(6/5)
BglII 5179 AGATCT A^GATCT
BlpI 3569 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3569 GCTGAGC GC^T[ACGT]AGC
BsaHI 609 GACGCC G[GA]^CG[CT]C
BsiEI 107 CGGCCG CG[GA][CT]^CG
BspHI 1732 TCATGA T^CATGA
BspMI 2824 ACCTGC ACCTGC(4/8)
BssSI 4719 CACGAG CACGAG(-5/-1)
CelII 3569 GCTGAGC GC^T[ACGT]AGC
DraIII 5521 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 107 CGGCCG C^GGCCG
Eam1105I 1987 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 107 CGGCCG C^GGCCG
Eco52I 107 CGGCCG C^GGCCG
EcoRI 2106 GAATTC G^AATTC
EcoRV 726 GATATC GAT^ATC
Hin1I 609 GACGCC G[GA]^CG[CT]C
HindIII 541 AAGCTT A^AGCTT
MaeII 5469 ACGT A^CGT
MfeI 1539 CAATTG C^AATTG
MunI 1539 CAATTG C^AATTG
NaeI 105 GCCGGC GCC^GGC
NgoMIV 105 GCCGGC G^CCGGC
PaeR7I 117 CTCGAG C^TCGAG
PflFI 1857 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2469 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1143 GACGCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5712 ATTAAT AT^TAAT
RcaI 1732 TCATGA T^CATGA
SalI 1140 GTCGAC G^TCGAC
SapI 1018 GAAGAGC GCTCTTC(1/4)
ScaI 3699 AGTACT AGT^ACT
SphI 4433 GCATGC GCATG^C
Tth111I 1857 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2469 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 117 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 505 ACATGT A^C[GA][CT]GT
5164 ACATGT
Asp700I 3291 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5209 GAAGTTTTTC
BamHI 1560 GGATCC G^GATCC
5385 GGATCC
BcgI 2487 CGATTCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2776 CGACCAAGCTGC
BsmI 3807 GAATGC GAATGC(1/-1)
4694 GCATTC
Bsp1407I 646 TGTACA T^GTACA
1846 TGTACA
BspLU11I 505 ACATGT A^CATGT
5164 ACATGT
BsrFI 105 GCCGGC [GA]^CCGG[CT]
2524 ACCGGC
BsrGI 646 TGTACA T^GTACA
1846 TGTACA
Bst1107I 261 GTATAC GTA^TAC
2342 GTATAC
BstZ17I 261 GTATAC GTA^TAC
2342 GTATAC
Cfr10I 105 GCCGGC [GA]^CCGG[CT]
2524 ACCGGC
DraI 408 TTTAAA TTT^AAA
4570 TTTAAA
DsaI 2955 CCATGG C^C[GA][CT]GG
3005 CCATGG
HincII 1140 GTCGAC GT[CT]^[GA]AC
1797 GTTGAC
HindII 1140 GTCGAC GT[CT]^[GA]AC
1797 GTTGAC
NcoI 2955 CCATGG C^CATGG
3005 CCATGG
PpuMI 973 GGGTCCC [GA]G^G[AT]CC[CT]
4282 GGGACCT
PstI 2799 CTGCAG CTGCA^G
2871 CTGCAG
SspBI 646 TGTACA T^GTACA
1846 TGTACA
SspI 3761 AATATT AAT^ATT
4529 AATATT
StuI 229 AGGCCT AGG^CCT
5410 AGGCCT
XmnI 3291 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5209 GAAGTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflII AluI AlwI AlwNI AocI
ApoI AspHI AvaI AvaII AvrII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BfrI BglI
BlnI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII