##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII AccII AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AspEI AviII AvrII BamHI BbvCI
BcgI BlnI BlpI Bpu1102I BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BsrBI BsrGI
BssHII BssSI BstAPI BstUI CelII
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
FseI FspI HindIII HpaI KspI
MluI MroI MvnI NaeI NgoMIV
NheI NotI NruI PacI PaeR7I
PflMI PinAI PmeI PpuMI Psp1406I
PstI PvuI RsrII SacII SbfI
SexAI SfiI SgrAI SmaI SnaBI
Sse8387I SspBI StuI Van91I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 4843 GGTACC G^GTACC
AocI 3174 CCTCAGG CC^T[ACGT]AGG
ApaI 3390 GGGCCC GGGCC^C
Asp718I 4843 GGTACC G^GTACC
AspI 5727 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 9 CCCGAG C^[CT]CG[GA]G
AvaII 2311 GGACC G^G[AT]CC
BbeI 5 GGCGCC GGCGC^C
BbrPI 4132 CACGTG CAC^GTG
BcnI 4146 CCCGG CC^[GC]GG
BglII 3636 AGATCT A^GATCT
BsaAI 4132 CACGTG [CT]AC^GT[GA]
BsaI 724 GGTCTC GGTCTC(1/5)
BsgI 4040 GTGCAG GTGCAG(16/14)
BsoBI 9 CCCGAG C^[CT]CG[GA]G
BspHI 138 TCATGA T^CATGA
BspMI 5809 ACCTGC ACCTGC(4/8)
BsrDI 239 CATTGC GCAATG(2/0)
BsrFI 659 ACCGGC [GA]^CCGG[CT]
Bst1107I 513 GTATAC GTA^TAC
BstBI 1402 TTCGAA TT^CGAA
BstEII 2407 GGTTACC G^GT[ACGT]ACC
BstPI 2407 GGTTACC G^GT[ACGT]ACC
BstYI 3636 AGATCT [GA]^GATC[CT]
BstZ17I 513 GTATAC GTA^TAC
Bsu36I 3174 CCTCAGG CC^T[ACGT]AGG
Cfr10I 659 ACCGGC [GA]^CCGG[CT]
Eco81I 3174 CCTCAGG CC^T[ACGT]AGG
EcoNI 1023 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2407 GGTTACC G^GT[ACGT]ACC
EcoT22I 3579 ATGCAT ATGCA^T
KasI 5 GGCGCC G^GCGCC
KpnI 4843 GGTACC GGTAC^C
MflI 3636 AGATCT [GA]^GATC[CT]
NarI 5 GGCGCC GG^CGCC
NciI 4146 CCCGG CC^[GC]GG
NcoI 4802 CCATGG C^CATGG
NdeI 2306 CATATG CA^TATG
NsiI 3579 ATGCAT ATGCA^T
NspV 1402 TTCGAA TT^CGAA
PflFI 5727 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4132 CACGTG CAC^GTG
PmlI 4132 CACGTG CAC^GTG
PshAI 2312 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 138 TCATGA T^CATGA
SalI 5447 GTCGAC G^TCGAC
ScaI 1114 AGTACT AGT^ACT
SfoI 5 GGCGCC GGC^GCC
SfuI 1402 TTCGAA TT^CGAA
SphI 5892 GCATGC GCATG^C
SwaI 5395 ATTTAAAT ATTT^AAAT
Tth111I 5727 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4590 TCTAGA T^CTAGA
XcmI 1284 CCAGCAGTTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 3636 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 5 GGCGCC G[GA]^CG[CT]C
614 GACGCC
AflIII 2072 ACATGT A^C[GA][CT]GT
4233 ACATGT
AlwI 4226 GATCC GGATC(4/5)
5623 GATCC
AlwNI 530 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
647 CAGAATCTG
AseI 3865 ATTAAT AT^TAAT
5363 ATTAAT
AsnI 3865 ATTAAT AT^TAAT
5363 ATTAAT
BglI 26 GCCACTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
266 GCCAAAATGGC
BsaHI 5 GGCGCC G[GA]^CG[CT]C
614 GACGCC
BspLU11I 2072 ACATGT A^CATGT
4233 ACATGT
DsaI 4382 CCGTGG C^C[GA][CT]GG
4802 CCATGG
EcoT14I 1583 CCAAGG C^C[AT][AT]GG
4802 CCATGG
HapII 660 CCGG C^CGG
4147 CCGG
HgaI 614 GACGC GACGC(5/10)
810 GCGTC
Hin1I 5 GGCGCC G[GA]^CG[CT]C
614 GACGCC
HpaII 660 CCGG C^CGG
4147 CCGG
MfeI 329 CAATTG C^AATTG
5642 CAATTG
MspI 660 CCGG C^CGG
4147 CCGG
MunI 329 CAATTG C^AATTG
5642 CAATTG
PshBI 3865 ATTAAT AT^TAAT
5363 ATTAAT
PvuII 186 CAGCTG CAG^CTG
1757 CAGCTG
SacI 2731 GAGCTC GAGCT^C
3124 GAGCTC
SpeI 1254 ACTAGT A^CTAGT
4504 ACTAGT
SspI 1092 AATATT AAT^ATT
5476 AATATT
StyI 1583 CCAAGG C^C[AT][AT]GG
4802 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AluI ApoI Asp700I AspHI BalI
BanI BanII BbsI BbvI BciVI
BclI BfaI BfrI BmyI BpmI
BpuAI BsaBI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EaeI EarI EcoO109I
EcoRI EcoRII FauI FbaI Fnu4HI
FokI HaeII HaeIII HhaI HinP1I
HincII HindII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XmnI