##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AclI AcyI AgeI AhdI
Alw44I Aor51HI ApaLI AscI AspEI
AviII AvrII BbeI BbrPI BcgI
BclI BlnI BsaAI BsaHI BsiEI
BsiWI BspLU11I BsrBI BssHII BstBI
CpoI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I KasI
MluI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflMI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacI SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 5669 GGTACC G^GTACC
AflII 5295 CTTAAG C^TTAAG
Asp718I 5669 GGTACC G^GTACC
BamHI 411 GGATCC G^GATCC
BciVI 1439 GTATCC GTATCC(6/5)
BfrI 5295 CTTAAG C^TTAAG
BsmBI 4431 CGTCTC CGTCTC(1/5)
BspDI 1492 ATCGAT AT^CGAT
BsrFI 3878 ACCGGC [GA]^CCGG[CT]
Bst1107I 5335 GTATAC GTA^TAC
BstEII 4880 GGTAACC G^GT[ACGT]ACC
BstPI 4880 GGTAACC G^GT[ACGT]ACC
BstZ17I 5335 GTATAC GTA^TAC
Cfr10I 3878 ACCGGC [GA]^CCGG[CT]
ClaI 1492 ATCGAT AT^CGAT
EcoO65I 4880 GGTAACC G^GT[ACGT]ACC
HincII 1516 GTTAAC GT[CT]^[GA]AC
HindII 1516 GTTAAC GT[CT]^[GA]AC
HpaI 1516 GTTAAC GTT^AAC
KpnI 5669 GGTACC GGTAC^C
MfeI 1953 CAATTG C^AATTG
MunI 1953 CAATTG C^AATTG
PaeR7I 4394 CTCGAG C^TCGAG
SapI 5268 GAAGAGC GCTCTTC(1/4)
SspI 346 AATATT AAT^ATT
StuI 777 AGGCCT AGG^CCT
SwaI 5783 ATTTAAAT ATTT^AAAT
XbaI 4017 TCTAGA T^CTAGA
XcmI 2099 CCAGCTATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 4394 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AccI 1941 GTCTAC GT^[AC][GT]AC
5335 GTATAC
AccIII 104 TCCGGA T^CCGGA
4841 TCCGGA
AflIII 4655 ACGTGT A^C[GA][CT]GT
5235 ACACGT
AocI 1455 CCTAAGG CC^T[ACGT]AGG
3042 CCTGAGG
AseI 1822 ATTAAT AT^TAAT
2857 ATTAAT
AsnI 1822 ATTAAT AT^TAAT
2857 ATTAAT
Asp700I 676 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3907 GAACGCATTC
AspHI 1279 GAGCAC G[AT]GC[AT]^C
5035 GAGCAC
AspI 1608 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4481 GACGTGGTC
AvaI 165 CCCGAG C^[CT]CG[GA]G
4394 CTCGAG
BbvCI 4379 CCTCAGC CCTCAGC(-5/-2)
2442 GCTGAGG
BglII 3668 AGATCT A^GATCT
4289 AGATCT
BlpI 1056 GCTCAGC GC^T[ACGT]AGC
5435 GCTAAGC
Bpu1102I 1056 GCTCAGC GC^T[ACGT]AGC
5435 GCTAAGC
BsaBI 2395 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4290 GATCTCCATC
BseAI 104 TCCGGA T^CCGGA
4841 TCCGGA
BsiHKAI 1279 GAGCAC G[AT]GC[AT]^C
5035 GAGCAC
BsoBI 165 CCCGAG C^[CT]CG[GA]G
4394 CTCGAG
Bsp1407I 5825 TGTACA T^GTACA
5903 TGTACA
BspEI 104 TCCGGA T^CCGGA
4841 TCCGGA
BsrGI 5825 TGTACA T^GTACA
5903 TGTACA
BstXI 3995 CCAAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4322 CCATTCCCCTGG
Bsu36I 1455 CCTAAGG CC^T[ACGT]AGG
3042 CCTGAGG
CelII 1056 GCTCAGC GC^T[ACGT]AGC
5435 GCTAAGC
Eco81I 1455 CCTAAGG CC^T[ACGT]AGG
3042 CCTGAGG
KspI 1 CCGCGG CCGC^GG
70 CCGCGG
MamI 2395 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4290 GATCTCCATC
MroI 104 TCCGGA T^CCGGA
4841 TCCGGA
MslI 3717 CATCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4251 CATTAGCGTG
NdeI 791 CATATG CA^TATG
5907 CATATG
NspI 689 ACATGC [GA]CATG^[CT]
4349 ACATGC
PflFI 1608 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4481 GACGTGGTC
PshAI 320 GACAAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
954 GACCAAAGTC
PshBI 1822 ATTAAT AT^TAAT
2857 ATTAAT
SacII 1 CCGCGG CCGC^GG
70 CCGCGG
ScaI 281 AGTACT AGT^ACT
764 AGTACT
SexAI 3167 ACCAGGT A^CC[AT]GGT
4184 ACCTGGT
SspBI 5825 TGTACA T^GTACA
5903 TGTACA
Tth111I 1608 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4481 GACGTGGTC
XmnI 676 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3907 GAACGCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaI BsaJI BsaWI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspHI BspMI BsrDI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DrdI DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII