##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
Acc65I AccIII AclI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AviII BamHI BbvCI
BcgI BseAI BsiWI BspDI BspEI
Bst1107I BstBI BstZ17I ClaI CpoI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoT22I FseI FspI
KpnI KspI MfeI MroI MunI
NotI NruI NsiI NspV PacI
PaeR7I PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfuI SgrAI SpeI Sse8387I SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AccI 356 GTCTAC GT^[AC][GT]AC
AgeI 1101 ACCGGT A^CCGGT
ApaI 4110 GGGCCC GGGCC^C
AvrII 4535 CCTAGG C^CTAGG
BglI 2502 GCCGTCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4535 CCTAGG C^CTAGG
BsiEI 2760 CGGTCG CG[GA][CT]^CG
BsmBI 1764 GAGACG CGTCTC(1/5)
BspLU11I 4253 ACATGT A^CATGT
BssHII 125 GCGCGC G^CGCGC
BssSI 2685 CACGAG CACGAG(-5/-1)
HpaI 548 GTTAAC GTT^AAC
MluI 5267 ACGCGT A^CGCGT
NaeI 1340 GCCGGC GCC^GGC
NdeI 4345 CATATG CA^TATG
NgoMIV 1340 GCCGGC G^CCGGC
NheI 5248 GCTAGC G^CTAGC
PinAI 1101 ACCGGT A^CCGGT
PshAI 4252 GACATGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1982 GCTCTTC GCTCTTC(1/4)
ScaI 679 AGTACT AGT^ACT
SmaI 3235 CCCGGG CCC^GGG
SnaBI 3659 TACGTA TAC^GTA
SphI 3878 GCATGC GCATG^C
StuI 1589 AGGCCT AGG^CCT
TaqI 1495 TCGA T^CGA
TthHB8I 1495 TCGA T^CGA
XmaCI 3235 CCCGGG C^CCGGG
XmaI 3235 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AatII 1503 GACGTC GACGT^C
2010 GACGTC
AflII 3043 CTTAAG C^TTAAG
3360 CTTAAG
Asp700I 2238 GAACTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4506 GAAATTATTC
AspI 1253 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1368 GACAAGGTC
AvaI 1220 CTCGGG C^[CT]CG[GA]G
3235 CCCGGG
BalI 1825 TGGCCA TGG^CCA
2361 TGGCCA
BbeI 2572 GGCGCC GGCGC^C
2590 GGCGCC
BbrPI 78 CACGTG CAC^GTG
5011 CACGTG
BfrI 3043 CTTAAG C^TTAAG
3360 CTTAAG
BglII 658 AGATCT A^GATCT
3179 AGATCT
BsaBI 2067 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5342 GATTATGATC
BsaWI 1101 ACCGGT [AT]^CCGG[AT]
4387 TCCGGT
BseRI 2517 CTCCTC GAGGAG(10/8)
1167 GAGGAG
BsgI 1839 CTGCAC GTGCAG(16/14)
3457 GTGCAG
BsmI 1410 GAATGC GAATGC(1/-1)
3888 GCATTC
BsoBI 1220 CTCGGG C^[CT]CG[GA]G
3235 CCCGGG
BsrBI 2952 CCGCTC CCGCTC(-3/-3)
1301 GAGCGG
BstAPI 824 GCATGACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2831 GCAGCCTTTGC
DrdI 1497 GACGGCGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2442 GACACAACTGTC
EcoRI 5066 GAATTC G^AATTC
5210 GAATTC
EcoRV 2622 GATATC GAT^ATC
5378 GATATC
HindIII 3593 AAGCTT A^AGCTT
4132 AAGCTT
KasI 2572 GGCGCC G^GCGCC
2590 GGCGCC
MamI 2067 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5342 GATTATGATC
MluNI 1825 TGGCCA TGG^CCA
2361 TGGCCA
MscI 1825 TGGCCA TGG^CCA
2361 TGGCCA
NarI 2572 GGCGCC GG^CGCC
2590 GGCGCC
NcoI 980 CCATGG C^CATGG
1928 CCATGG
PflFI 1253 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1368 GACAAGGTC
PmaCI 78 CACGTG CAC^GTG
5011 CACGTG
PmeI 3319 GTTTAAAC GTTT^AAAC
3556 GTTTAAAC
PmlI 78 CACGTG CAC^GTG
5011 CACGTG
SacI 1437 GAGCTC GAGCT^C
1980 GAGCTC
SfoI 2572 GGCGCC GGC^GCC
2590 GGCGCC
SmlI 3043 CTTAAG C^T[CT][GA]AG
3360 CTTAAG
Tth111I 1253 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1368 GACAAGGTC
XbaI 4734 TCTAGA T^CTAGA
4881 TCTAGA
XcmI 652 CCAATGAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3340 CCACACAATGGATGG
XmnI 2238 GAACTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4506 GAAATTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI AspHI AvaII BanI BanII
BbsI BbvI BciVI BclI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BspHI BspMI BsrDI
BsrFI BsrGI BsrI BssKI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoO65I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspBI SspI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XhoII