##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BbeI BbvCI BcgI BfrI
BlnI BsaBI BsaWI BseAI BsiWI
BspDI BspEI BspLU11I BsrFI Bst1107I
BstBI BstZ17I Cfr10I ClaI CpoI
DraIII Eam1105I Eco47III EcoRI EcoRV
EcoT22I FseI FspI HaeII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI NaeI
NarI NdeI NgoMIV NheI NsiI
NspV PacI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI SwaI XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccI 2610 GTCTAC GT^[AC][GT]AC
AcsI 152 AAATTT [GA]^AATT[CT]
AcyI 1478 GACGCC G[GA]^CG[CT]C
Alw44I 363 GTGCAC G^TGCAC
AocI 1156 CCTAAGG CC^T[ACGT]AGG
ApaLI 363 GTGCAC G^TGCAC
ApoI 152 AAATTT [GA]^AATT[CT]
AspI 501 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 876 CTCGAG C^[CT]CG[GA]G
BalI 1827 TGGCCA TGG^CCA
BamHI 862 GGATCC G^GATCC
BbrPI 1580 CACGTG CAC^GTG
BbsI 256 GAAGAC GAAGAC(2/6)
BciVI 698 GTATCC GTATCC(6/5)
BclI 658 TGATCA T^GATCA
BglI 1626 GCCACCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2064 AGATCT A^GATCT
BlpI 2314 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2314 GCTTAGC GC^T[ACGT]AGC
BpuAI 256 GAAGAC GAAGAC(2/6)
BsaAI 1580 CACGTG [CT]AC^GT[GA]
BsaHI 1478 GACGCC G[GA]^CG[CT]C
BsaI 506 GGTCTC GGTCTC(1/5)
BsiEI 2 CGGCCG CG[GA][CT]^CG
BsmBI 1036 CGTCTC CGTCTC(1/5)
BsoBI 876 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1584 TGTACA T^GTACA
BspHI 2176 TCATGA T^CATGA
BsrBI 1248 GAGCGG CCGCTC(-3/-3)
BsrGI 1584 TGTACA T^GTACA
BssHII 41 GCGCGC G^CGCGC
BssSI 1573 CACGAG CACGAG(-5/-1)
BstAPI 1292 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1930 CCACACAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1156 CCTAAGG CC^T[ACGT]AGG
CelII 2314 GCTTAGC GC^T[ACGT]AGC
DrdI 1997 GACAACACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2 CGGCCG C^GGCCG
EclXI 2 CGGCCG C^GGCCG
Eco52I 2 CGGCCG C^GGCCG
Eco81I 1156 CCTAAGG CC^T[ACGT]AGG
FbaI 658 TGATCA T^GATCA
Hin1I 1478 GACGCC G[GA]^CG[CT]C
MluNI 1827 TGGCCA TGG^CCA
MscI 1827 TGGCCA TGG^CCA
NcoI 441 CCATGG C^CATGG
NotI 1 GCGGCCGC GC^GGCCGC
NruI 85 TCGCGA TCG^CGA
PaeR7I 876 CTCGAG C^TCGAG
PflFI 501 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1580 CACGTG CAC^GTG
PmlI 1580 CACGTG CAC^GTG
RcaI 2176 TCATGA T^CATGA
SacI 688 GAGCTC GAGCT^C
SapI 690 GCTCTTC GCTCTTC(1/4)
SphI 1507 GCATGC GCATG^C
SspBI 1584 TGTACA T^GTACA
StuI 1109 AGGCCT AGG^CCT
Tth111I 501 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 876 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 1579 ACACGT A^C[GA][CT]GT
1581 ACGTGT
AlwNI 1293 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
2008 CAGGTCCTG
Asp700I 188 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2702 GAAGGCTTTC
BanI 124 GGCACC G^G[CT][GA]CC
1970 GGCACC
BfaI 605 CTAG C^TAG
914 CTAG
BsgI 1895 CTGCAC GTGCAG(16/14)
410 GTGCAG
BspMI 2019 ACCTGC ACCTGC(4/8)
433 GCAGGT
BsrDI 2509 CATTGC GCAATG(2/0)
2124 GCAATG
BstEII 436 GGTGACC G^GT[ACGT]ACC
1555 GGTGACC
BstPI 436 GGTGACC G^GT[ACGT]ACC
1555 GGTGACC
DraI 149 TTTAAA TTT^AAA
2696 TTTAAA
EaeI 2 CGGCCG [CT]^GGCC[GA]
1827 TGGCCA
EcoNI 1908 CCTTGGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2020 CCTGCGCGAGG
EcoO65I 436 GGTGACC G^GT[ACGT]ACC
1555 GGTGACC
HgaI 1478 GACGC GACGC(5/10)
1605 GCGTC
HincII 845 GTTGAC GT[CT]^[GA]AC
1136 GTTGAC
HindII 845 GTTGAC GT[CT]^[GA]AC
1136 GTTGAC
MaeI 605 CTAG C^TAG
914 CTAG
MaeII 849 ACGT A^CGT
1581 ACGT
PflMI 1903 CCAGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1974 CCAAGCTCTGG
ScaI 980 AGTACT AGT^ACT
1742 AGTACT
TaqI 877 TCGA T^CGA
1659 TCGA
TthHB8I 877 TCGA T^CGA
1659 TCGA
Van91I 1903 CCAGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1974 CCAAGCTCTGG
XcmI 1752 CCATTTGGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2087 CCAACTGGAGAGTGG
XmnI 188 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2702 GAAGGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaII BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII