##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

AatII       AccIII      AhdI        AlwNI       AscI        
AspEI       AviII       AvrII       BamHI       BcgI        
BglI        BlnI        BsaBI       BseAI       BsiEI       
BsiWI       Bsp1407I    BspDI       BspEI       BsrGI       
BssHII      Bst1107I    BstBI       BstZ17I     ClaI        
CpoI        DraIII      DsaI        EagI        Eam1105I    
EclXI       Eco52I      EcoRV       EcoT22I     FseI        
FspI        KspI        MamI        MfeI        MluI        
MroI        MunI        NcoI        NdeI        NheI        
NotI        NsiI        NspV        PacI        PaeR7I      
PmeI        PvuI        RsrII       SacII       SalI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SspBI       SwaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

Acc65I       4184  GGTACC               G^GTACC
AccI         4114  GTAGAC               GT^[AC][GT]AC
AccII         898  CGCG                 CG^CG
AclI          314  AACGTT               AA^CGTT
AcyI         5556  GGCGCC               G[GA]^CG[CT]C
AgeI         1001  ACCGGT               A^CCGGT
Alw44I        839  GTGCAC               G^TGCAC
AocI         4061  CCTTAGG              CC^T[ACGT]AGG
Aor51HI      2915  AGCGCT               AGC^GCT
ApaI          887  GGGCCC               GGGCC^C
ApaLI         839  GTGCAC               G^TGCAC
AseI         3957  ATTAAT               AT^TAAT
AsnI         3957  ATTAAT               AT^TAAT
Asp718I      4184  GGTACC               G^GTACC
BbeI         5556  GGCGCC               GGCGC^C
BcnI         2604  CCGGG                CC^[GC]GG
BlpI         2248  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2248  GCTGAGC              GC^T[ACGT]AGC
BsaHI        5556  GGCGCC               G[GA]^CG[CT]C
BsaI          592  GGTCTC               GGTCTC(1/5)
BsmBI        1966  GAGACG               CGTCTC(1/5)
BspLU11I      829  ACATGT               A^CATGT
BsrBI        3220  CCGCTC               CCGCTC(-3/-3)
BstAPI       1351  GCAATTCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       4056  GGTAACC              G^GT[ACGT]ACC
BstPI        4056  GGTAACC              G^GT[ACGT]ACC
BstUI         898  CGCG                 CG^CG
Bsu36I       4061  CCTTAGG              CC^T[ACGT]AGG
CelII        2248  GCTGAGC              GC^T[ACGT]AGC
DrdI         1392  GACAACCCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     2915  AGCGCT               AGC^GCT
Eco81I       4061  CCTTAGG              CC^T[ACGT]AGG
EcoO65I      4056  GGTAACC              G^GT[ACGT]ACC
HgaI          565  GACGC                GACGC(5/10)
Hin1I        5556  GGCGCC               G[GA]^CG[CT]C
HpaI         2800  GTTAAC               GTT^AAC
KasI         5556  GGCGCC               G^GCGCC
KpnI         4184  GGTACC               GGTAC^C
MvnI          898  CGCG                 CG^CG
NaeI         3638  GCCGGC               GCC^GGC
NarI         5556  GGCGCC               GG^CGCC
NciI         2604  CCGGG                CC^[GC]GG
NgoMIV       3638  GCCGGC               G^CCGGC
NruI          897  TCGCGA               TCG^CGA
PinAI        1001  ACCGGT               A^CCGGT
PpuMI         332  AGGACCT              [GA]G^G[AT]CC[CT]
PshAI        2901  GACCACCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        3957  ATTAAT               AT^TAAT
Psp1406I      314  AACGTT               AA^CGTT
SbfI         2094  CCTGCAGG             CCTGCA^GG
SfoI         5556  GGCGCC               GGC^GCC
Sse8387I     2094  CCTGCAGG             CCTGCA^GG
StuI         4652  AGGCCT               AGG^CCT
XcmI         4418  CCACACTCAGACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AspI          571  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2739  GACTTCGTC           
AvaI         5576  CTCGGG               C^[CT]CG[GA]G
             5608  CTCGGG              
BbvCI        4637  CCTCAGC              CCTCAGC(-5/-2)
             2043  GCTGAGG             
BciVI        4940  GTATCC               GTATCC(6/5)
             5334  GTATCC              
BclI         3027  TGATCA               T^GATCA
             5074  TGATCA              
BsaWI        1001  ACCGGT               [AT]^CCGG[AT]
             2860  ACCGGA              
BsoBI        5576  CTCGGG               C^[CT]CG[GA]G
             5608  CTCGGG              
BssSI        1693  CACGAG               CACGAG(-5/-1)
             3621  CACGAG              
BstXI        1448  CCACCGACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5436  CCAGCACTTTGG        
DraI         4078  TTTAAA               TTT^AAA
             4954  TTTAAA              
DraII         332  AGGACCT              [GA]G^G[ACGT]CC[CT]
              452  AGGCCCT             
EcoO109I      332  AGGACCT              [GA]G^G[ACGT]CC[CT]
              452  AGGCCCT             
EcoRI        4014  GAATTC               G^AATTC
             4495  GAATTC              
FbaI         3027  TGATCA               T^GATCA
             5074  TGATCA              
HaeII        2915  AGCGCT               [GA]GCGC^[CT]
             5556  GGCGCC              
PflFI         571  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2739  GACTTCGTC           
SapI          427  GAAGAGC              GCTCTTC(1/4)
             3211  GAAGAGC             
SphI         3152  GCATGC               GCATG^C
             3176  GCATGC              
SspI         3377  AATATT               AAT^ATT
             4533  AATATT              
Tth111I       571  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2739  GACTTCGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  117 enzymes) #
#########################################

AciI        AcsI        AfaI        AflII       AflIII      
AluI        AlwI        ApoI        Asp700I     AspHI       
AvaII       BalI        BanI        BanII       BbrPI       
BbsI        BbvI        BfaI        BfrI        BglII       
BmyI        BpmI        BpuAI       BsaAI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        Bsp1286I    BspHI       
BspMI       BsrDI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       EaeI        
EarI        EcoNI       EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HincII      HindII      HindIII     HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PmaCI       PmlI        PstI        PvuII       RcaI        
RsaI        SacI        Sau3AI      Sau96I      ScaI        
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XbaI        
XhoII       XmnI