##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AclI AcyI AflII AgeI
AscI AspI AviII AvrII BbeI
BbrPI BciVI BfrI BglI BlnI
BlpI Bpu1102I BsaHI BsgI BspDI
BsrBI BsrDI BssHII BssSI BstBI
BstEII BstPI CelII ClaI CpoI
DrdI EagI EclXI Eco52I EcoO65I
EcoRV FseI FspI HgaI Hin1I
HindIII KasI KspI MluI NarI
NcoI NdeI NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I Tth111I
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 1674 GGTACC G^GTACC
AccI 3463 GTATAC GT^[AC][GT]AC
AccII 904 CGCG CG^CG
AccIII 1992 TCCGGA T^CCGGA
AhdI 2420 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3411 GTGCAC G^TGCAC
ApaI 1603 GGGCCC GGGCC^C
ApaLI 3411 GTGCAC G^TGCAC
Asp718I 1674 GGTACC G^GTACC
AspEI 2420 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BclI 2034 TGATCA T^GATCA
BglII 3964 AGATCT A^GATCT
BsaAI 1058 CACGTA [CT]AC^GT[GA]
BsaWI 1992 TCCGGA [AT]^CCGG[AT]
BseAI 1992 TCCGGA T^CCGGA
BsiWI 20 CGTACG C^GTACG
BsmBI 2005 GAGACG CGTCTC(1/5)
Bsp1407I 2073 TGTACA T^GTACA
BspEI 1992 TCCGGA T^CCGGA
BspMI 2654 GCAGGT ACCTGC(4/8)
BsrGI 2073 TGTACA T^GTACA
Bst1107I 3463 GTATAC GTA^TAC
BstUI 904 CGCG CG^CG
BstZ17I 3463 GTATAC GTA^TAC
DraIII 1519 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2420 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3688 GAATTC G^AATTC
FbaI 2034 TGATCA T^GATCA
HpaI 4039 GTTAAC GTT^AAC
KpnI 1674 GGTACC GGTAC^C
MfeI 2814 CAATTG C^AATTG
MroI 1992 TCCGGA T^CCGGA
MunI 2814 CAATTG C^AATTG
MvnI 904 CGCG CG^CG
NaeI 269 GCCGGC GCC^GGC
NgoMIV 269 GCCGGC G^CCGGC
NheI 3769 GCTAGC G^CTAGC
PaeR7I 1213 CTCGAG C^TCGAG
PvuI 1546 CGATCG CGAT^CG
SapI 4079 GAAGAGC GCTCTTC(1/4)
ScaI 3182 AGTACT AGT^ACT
SexAI 3890 ACCAGGT A^CC[AT]GGT
SmaI 191 CCCGGG CCC^GGG
SphI 66 GCATGC GCATG^C
SspBI 2073 TGTACA T^GTACA
SwaI 4198 ATTTAAAT ATTT^AAAT
XhoI 1213 CTCGAG C^TCGAG
XmaCI 191 CCCGGG C^CCGGG
XmaI 191 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 2070 ACATGT A^C[GA][CT]GT
3025 ACATGT
AocI 844 CCTAAGG CC^T[ACGT]AGG
2381 CCTGAGG
Aor51HI 220 AGCGCT AGC^GCT
4213 AGCGCT
BalI 1893 TGGCCA TGG^CCA
3601 TGGCCA
BamHI 1015 GGATCC G^GATCC
2508 GGATCC
BcgI 952 CGATACTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2708 GCAGAGGATTCG
BsaBI 1010 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1541 GATTACGATC
BsiEI 972 CGACCG CG[GA][CT]^CG
1546 CGATCG
BspHI 2682 TCATGA T^CATGA
4485 TCATGA
BspLU11I 2070 ACATGT A^CATGT
3025 ACATGT
BstAPI 1929 GCATCGTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4737 GCAGAAACTGC
BstXI 729 CCATCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2809 CCAGCCAATTGG
Bsu36I 844 CCTAAGG CC^T[ACGT]AGG
2381 CCTGAGG
Eco47III 220 AGCGCT AGC^GCT
4213 AGCGCT
Eco81I 844 CCTAAGG CC^T[ACGT]AGG
2381 CCTGAGG
HincII 2066 GTTGAC GT[CT]^[GA]AC
4039 GTTAAC
HindII 2066 GTTGAC GT[CT]^[GA]AC
4039 GTTAAC
MamI 1010 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1541 GATTACGATC
MluNI 1893 TGGCCA TGG^CCA
3601 TGGCCA
MscI 1893 TGGCCA TGG^CCA
3601 TGGCCA
PpuMI 1318 GGGACCC [GA]G^G[AT]CC[CT]
2538 AGGACCT
RcaI 2682 TCATGA T^CATGA
4485 TCATGA
StuI 1083 AGGCCT AGG^CCT
2791 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI Asp700I
AspHI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NsiI NspI PflMI PleI
PshBI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XcmI XhoII
XmnI