##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AccII AccIII AflII AgeI
AhdI Alw44I AocI ApaI ApaLI
AscI AspEI AspI AviII AvrII
BbeI BbrPI BcgI BfrI BlnI
BlpI BpmI Bpu1102I BsaWI BseAI
BsiEI BsiWI BspDI BspEI BsrFI
BssHII BssSI BstBI BstEII BstPI
BstUI Bsu36I CelII Cfr10I ClaI
CpoI DraII DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoO65I FseI FspI HpaI KasI
KspI MluI MroI MvnI NaeI
NarI NgoMIV NotI NruI NspV
PacI PflFI PflMI PinAI PmaCI
PmlI PpuMI PshAI PvuI RsrII
SacI SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I StuI Tth111I Van91I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AclI 4845 AACGTT AA^CGTT
AcyI 1438 GACGCC G[GA]^CG[CT]C
Aor51HI 180 AGCGCT AGC^GCT
AvaI 56 CTCGAG C^[CT]CG[GA]G
BalI 390 TGGCCA TGG^CCA
BamHI 2050 GGATCC G^GATCC
BanII 2131 GGGCTC G[GA]GC[CT]^C
BbvCI 1983 GCTGAGG CCTCAGC(-5/-2)
BciVI 511 GTATCC GTATCC(6/5)
BcnI 16 CCGGG CC^[GC]GG
BglI 2293 GCCATGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 2745 CACGTA [CT]AC^GT[GA]
BsaHI 1438 GACGCC G[GA]^CG[CT]C
BsaI 724 GAGACC GGTCTC(1/5)
BsmBI 1436 GAGACG CGTCTC(1/5)
BsmFI 2797 GGGAC GGGAC(10/14)
BsoBI 56 CTCGAG C^[CT]CG[GA]G
BspMI 3191 ACCTGC ACCTGC(4/8)
BsrBI 31 GAGCGG CCGCTC(-3/-3)
Bst1107I 2275 GTATAC GTA^TAC
BstXI 2516 CCATTATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2275 GTATAC GTA^TAC
CfoI 181 GCGC GCG^C
DraIII 4867 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 390 TGGCCA [CT]^GGCC[GA]
Eco47III 180 AGCGCT AGC^GCT
EcoRI 4763 GAATTC G^AATTC
EcoRV 4413 GATATC GAT^ATC
FauI 3359 GCGGG CCCGC(4/6)
HaeII 180 AGCGCT [GA]GCGC^[CT]
HgaI 1438 GACGC GACGC(5/10)
HhaI 181 GCGC GCG^C
Hin1I 1438 GACGCC G[GA]^CG[CT]C
HinP1I 181 GCGC G^CGC
HincII 5157 GTCAAC GT[CT]^[GA]AC
HindII 5157 GTCAAC GT[CT]^[GA]AC
MluNI 390 TGGCCA TGG^CCA
MscI 390 TGGCCA TGG^CCA
NciI 16 CCGGG CC^[GC]GG
NheI 3959 GCTAGC G^CTAGC
PaeR7I 56 CTCGAG C^TCGAG
PmeI 3185 GTTTAAAC GTTT^AAAC
Psp1406I 4845 AACGTT AA^CGTT
SphI 2312 GCATGC GCATG^C
SspI 2941 AATATT AAT^ATT
XhoI 56 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 25 GGTACC G^GTACC
3678 GGTACC
AccI 2275 GTATAC GT^[AC][GT]AC
3072 GTAGAC
AflIII 1020 ACATGT A^C[GA][CT]GT
3552 ACATGT
AseI 373 ATTAAT AT^TAAT
4293 ATTAAT
AsnI 373 ATTAAT AT^TAAT
4293 ATTAAT
Asp718I 25 GGTACC G^GTACC
3678 GGTACC
AspHI 2998 GTGCTC G[AT]GC[AT]^C
3548 GAGCAC
AvaII 873 GGTCC G^G[AT]CC
5045 GGTCC
BbsI 2194 GTCTTC GAAGAC(2/6)
3917 GTCTTC
BglII 963 AGATCT A^GATCT
1056 AGATCT
BpuAI 2194 GTCTTC GAAGAC(2/6)
3917 GTCTTC
BsiHKAI 2998 GTGCTC G[AT]GC[AT]^C
3548 GAGCAC
BspLU11I 1020 ACATGT A^CATGT
3552 ACATGT
EarI 4078 CTCTTC CTCTTC(1/4)
1876 GAAGAG
HapII 16 CCGG C^CGG
1772 CCGG
HindIII 1708 AAGCTT A^AGCTT
2770 AAGCTT
HpaII 16 CCGG C^CGG
1772 CCGG
KpnI 25 GGTACC GGTAC^C
3678 GGTACC
Ksp632I 4078 CTCTTC CTCTTC(1/4)
1876 GAAGAG
MfeI 2138 CAATTG C^AATTG
2270 CAATTG
MspI 16 CCGG C^CGG
1772 CCGG
MunI 2138 CAATTG C^AATTG
2270 CAATTG
PleI 1783 GACTC GAGTC(4/5)
2799 GACTC
PshBI 373 ATTAAT AT^TAAT
4293 ATTAAT
PstI 2378 CTGCAG CTGCA^G
3193 CTGCAG
SexAI 822 ACCAGGT A^CC[AT]GGT
1256 ACCTGGT
SmlI 56 CTCGAG C^T[CT][GA]AG
1759 CTCAAG
SwaI 479 ATTTAAAT ATTT^AAAT
3703 ATTTAAAT
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I BanI BbvI
BclI BfaI BmyI BsaBI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmI Bsp1286I Bsp1407I BspHI BsrDI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DsaI EcoRII EcoT14I
EcoT22I FbaI Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NcoI NdeI NdeII NlaIII
NlaIV NsiI NspI PvuII RcaI
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SpeI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XcmI
XhoII XmnI