##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccIII AclI AgeI Alw44I
ApaI ApaLI AscI AseI AsnI
Asp700I AspI BbrPI BcgI BglII
BseAI BsiWI BsmI BspDI BspEI
BspLU11I BssHII Bst1107I BstBI BstEII
BstPI BstXI BstZ17I ClaI CpoI
DrdI EcoNI EcoO65I EcoRV FseI
KspI MluI MroI NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
StuI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AccII 2720 CGCG CG^CG
AcyI 106 GGCGCC G[GA]^CG[CT]C
AflIII 1871 ACACGT A^C[GA][CT]GT
AhdI 72 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3294 CCTCAGG CC^T[ACGT]AGG
AspEI 72 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1991 TGCGCA TGC^GCA
AvrII 3059 CCTAGG C^CTAGG
BalI 4515 TGGCCA TGG^CCA
BamHI 265 GGATCC G^GATCC
BbeI 106 GGCGCC GGCGC^C
BbvCI 1793 GCTGAGG CCTCAGC(-5/-2)
BciVI 3142 GGATAC GTATCC(6/5)
BlnI 3059 CCTAGG C^CTAGG
BpmI 853 CTCCAG CTGGAG(16/14)
BsaAI 1512 TACGTG [CT]AC^GT[GA]
BsaHI 106 GGCGCC G[GA]^CG[CT]C
BsiEI 2950 CGGCCG CG[GA][CT]^CG
Bsp1407I 4136 TGTACA T^GTACA
BspHI 601 TCATGA T^CATGA
BsrDI 253 CATTGC GCAATG(2/0)
BsrFI 811 GCCGGC [GA]^CCGG[CT]
BsrGI 4136 TGTACA T^GTACA
BssSI 2899 CACGAG CACGAG(-5/-1)
BstAPI 4672 GCATAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 2720 CGCG CG^CG
Bsu36I 3294 CCTCAGG CC^T[ACGT]AGG
Cfr10I 811 GCCGGC [GA]^CCGG[CT]
DraI 4636 TTTAAA TTT^AAA
DraIII 1995 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2950 CGGCCG C^GGCCG
Eam1105I 72 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2950 CGGCCG C^GGCCG
Eco52I 2950 CGGCCG C^GGCCG
Eco81I 3294 CCTCAGG CC^T[ACGT]AGG
EcoRI 1479 GAATTC G^AATTC
EcoT22I 4670 ATGCAT ATGCA^T
FspI 1991 TGCGCA TGC^GCA
Hin1I 106 GGCGCC G[GA]^CG[CT]C
HincII 4443 GTTAAC GT[CT]^[GA]AC
HindII 4443 GTTAAC GT[CT]^[GA]AC
HindIII 3457 AAGCTT A^AGCTT
HpaI 4443 GTTAAC GTT^AAC
KasI 106 GGCGCC G^GCGCC
MfeI 712 CAATTG C^AATTG
MluNI 4515 TGGCCA TGG^CCA
MscI 4515 TGGCCA TGG^CCA
MunI 712 CAATTG C^AATTG
MvnI 2720 CGCG CG^CG
NaeI 811 GCCGGC GCC^GGC
NarI 106 GGCGCC GG^CGCC
NdeI 3493 CATATG CA^TATG
NgoMIV 811 GCCGGC G^CCGGC
NsiI 4670 ATGCAT ATGCA^T
PshAI 1386 GACGCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 601 TCATGA T^CATGA
SacI 2665 GAGCTC GAGCT^C
SapI 653 GCTCTTC GCTCTTC(1/4)
SexAI 841 ACCTGGT A^CC[AT]GGT
SfoI 106 GGCGCC GGC^GCC
SspBI 4136 TGTACA T^GTACA
SspI 4168 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
Acc65I 1546 GGTACC G^GTACC
4105 GGTACC
AccI 1696 GTCTAC GT^[AC][GT]AC
4542 GTAGAC
Aor51HI 1002 AGCGCT AGC^GCT
2400 AGCGCT
Asp718I 1546 GGTACC G^GTACC
4105 GGTACC
BclI 598 TGATCA T^GATCA
4468 TGATCA
BsaBI 678 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1976 GATGTGAATC
BsaWI 147 TCCGGT [AT]^CCGG[AT]
1947 ACCGGA
BsmBI 1831 CGTCTC CGTCTC(1/5)
1384 GAGACG
Eco47III 1002 AGCGCT AGC^GCT
2400 AGCGCT
FbaI 598 TGATCA T^GATCA
4468 TGATCA
KpnI 1546 GGTACC GGTAC^C
4105 GGTACC
MamI 678 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1976 GATGTGAATC
NspI 2142 GCATGC [GA]CATG^[CT]
2854 GCATGC
SphI 2142 GCATGC GCATG^C
2854 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BfrI BglI BlpI
BmyI Bpu1102I BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII