##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AhdI Aor51HI AscI Asp718I AspEI
AviII BcgI BfrI BglII BsiWI
BsmI BspDI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraIII DrdI Eam1105I Eco47III EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HindIII HpaI KpnI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PmeI Psp1406I PvuI RsrII
SacI SalI SapI SfuI SgrAI
SnaBI SpeI SspI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccIII 134 TCCGGA T^CCGGA
AcsI 465 GAATTT [GA]^AATT[CT]
AgeI 1865 ACCGGT A^CCGGT
AocI 1611 CCTGAGG CC^T[ACGT]AGG
ApoI 465 GAATTT [GA]^AATT[CT]
Asp700I 3647 GAATTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 2161 GGATCC G^GATCC
BciVI 1368 GGATAC GTATCC(6/5)
BclI 1959 TGATCA T^GATCA
BsaBI 1050 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 134 TCCGGA T^CCGGA
BsmBI 2959 CGTCTC CGTCTC(1/5)
Bsp1407I 3286 TGTACA T^GTACA
BspEI 134 TCCGGA T^CCGGA
BspLU11I 2356 ACATGT A^CATGT
BsrDI 3058 GCAATG GCAATG(2/0)
BsrGI 3286 TGTACA T^GTACA
BstAPI 1593 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1611 CCTGAGG CC^T[ACGT]AGG
Eco81I 1611 CCTGAGG CC^T[ACGT]AGG
FbaI 1959 TGATCA T^GATCA
MamI 1050 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1576 CAATTG C^AATTG
MluI 1429 ACGCGT A^CGCGT
MroI 134 TCCGGA T^CCGGA
MunI 1576 CAATTG C^AATTG
PinAI 1865 ACCGGT A^CCGGT
PshAI 1121 GACGCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 2043 CCTGCAGG CCTGCA^GG
ScaI 3926 AGTACT AGT^ACT
SexAI 955 ACCTGGT A^CC[AT]GGT
SfiI 1184 GGCCGCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 291 CTCAAG C^T[CT][GA]AG
Sse8387I 2043 CCTGCAGG CCTGCA^GG
SspBI 3286 TGTACA T^GTACA
XmnI 3647 GAATTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflIII 1429 ACGCGT A^C[GA][CT]GT
2356 ACATGT
AseI 1933 ATTAAT AT^TAAT
3649 ATTAAT
AsnI 1933 ATTAAT AT^TAAT
3649 ATTAAT
AspI 582 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
819 GACGAGGTC
BalI 973 TGGCCA TGG^CCA
2672 TGGCCA
BbrPI 1165 CACGTG CAC^GTG
1777 CACGTG
BbvCI 1097 CCTCAGC CCTCAGC(-5/-2)
2891 GCTGAGG
BlpI 73 GCTGAGC GC^T[ACGT]AGC
2137 GCTAAGC
Bpu1102I 73 GCTGAGC GC^T[ACGT]AGC
2137 GCTAAGC
BsaWI 134 TCCGGA [AT]^CCGG[AT]
1865 ACCGGT
BspHI 1111 TCATGA T^CATGA
2124 TCATGA
CelII 73 GCTGAGC GC^T[ACGT]AGC
2137 GCTAAGC
DraI 3300 TTTAAA TTT^AAA
4134 TTTAAA
HincII 3273 GTTGAC GT[CT]^[GA]AC
3496 GTCAAC
HindII 3273 GTTGAC GT[CT]^[GA]AC
3496 GTCAAC
MluNI 973 TGGCCA TGG^CCA
2672 TGGCCA
MscI 973 TGGCCA TGG^CCA
2672 TGGCCA
NaeI 807 GCCGGC GCC^GGC
2540 GCCGGC
NgoMIV 807 GCCGGC G^CCGGC
2540 GCCGGC
PflFI 582 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
819 GACGAGGTC
PflMI 20 CCAGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
842 CCAGGAGCTGG
PmaCI 1165 CACGTG CAC^GTG
1777 CACGTG
PmlI 1165 CACGTG CAC^GTG
1777 CACGTG
PshBI 1933 ATTAAT AT^TAAT
3649 ATTAAT
RcaI 1111 TCATGA T^CATGA
2124 TCATGA
SphI 946 GCATGC GCATG^C
3334 GCATGC
TaqI 905 TCGA T^CGA
2634 TCGA
Tth111I 582 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
819 GACGAGGTC
TthHB8I 905 TCGA T^CGA
2634 TCGA
Van91I 20 CCAGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
842 CCAGGAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaI ApaLI
AspHI AvaI AvaII AvrII BanI
BanII BbeI BbsI BbvI BcnI
BfaI BglI BlnI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XcmI XhoII XmaCI
XmaI