##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AgeI AhdI Alw44I ApaLI ApoI
AscI AseI AsnI AspEI AviII
BbeI BbrPI BclI BfrI BseAI
BsiWI BsmBI BsmI BspDI BspEI
BspLU11I BssHII BssSI ClaI CpoI
DrdI Eam1105I EcoRI EcoRV FbaI
FseI FspI HincII HindII HindIII
HpaI KasI MluI MroI NaeI
NarI NdeI NgoMIV NotI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacI
SalI SapI SbfI ScaI SfoI
SgrAI SmlI SpeI SphI Sse8387I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 1128 GGTACC G^GTACC
AccI 1617 GTATAC GT^[AC][GT]AC
AflIII 303 ACGTGT A^C[GA][CT]GT
AocI 490 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1661 AGCGCT AGC^GCT
Asp700I 240 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1128 GGTACC G^GTACC
AspHI 2737 GTGCTC G[AT]GC[AT]^C
AvrII 1116 CCTAGG C^CTAGG
BcgI 3415 GCAGAAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 642 GGATAC GTATCC(6/5)
BglII 1091 AGATCT A^GATCT
BlnI 1116 CCTAGG C^CTAGG
BsaAI 2267 TACGTA [CT]AC^GT[GA]
BsaWI 2893 ACCGGA [AT]^CCGG[AT]
BsiHKAI 2737 GTGCTC G[AT]GC[AT]^C
Bsp1407I 2084 TGTACA T^GTACA
BspHI 3290 TCATGA T^CATGA
BsrFI 170 ACCGGC [GA]^CCGG[CT]
BsrGI 2084 TGTACA T^GTACA
Bst1107I 1617 GTATAC GTA^TAC
BstAPI 771 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 50 TTCGAA TT^CGAA
BstEII 523 GGTGACC G^GT[ACGT]ACC
BstPI 523 GGTGACC G^GT[ACGT]ACC
BstZ17I 1617 GTATAC GTA^TAC
Bsu36I 490 CCTGAGG CC^T[ACGT]AGG
Cfr10I 170 ACCGGC [GA]^CCGG[CT]
EarI 3957 GAAGAG CTCTTC(1/4)
Eco47III 1661 AGCGCT AGC^GCT
Eco81I 490 CCTGAGG CC^T[ACGT]AGG
EcoO65I 523 GGTGACC G^GT[ACGT]ACC
EcoT22I 2198 ATGCAT ATGCA^T
KpnI 1128 GGTACC GGTAC^C
Ksp632I 3957 GAAGAG CTCTTC(1/4)
KspI 201 CCGCGG CCGC^GG
MfeI 408 CAATTG C^AATTG
MunI 408 CAATTG C^AATTG
NheI 592 GCTAGC G^CTAGC
NruI 2872 TCGCGA TCG^CGA
NsiI 2198 ATGCAT ATGCA^T
NspV 50 TTCGAA TT^CGAA
RcaI 3290 TCATGA T^CATGA
SacII 201 CCGCGG CCGC^GG
SfuI 50 TTCGAA TT^CGAA
SmaI 1775 CCCGGG CCC^GGG
SnaBI 2267 TACGTA TAC^GTA
SspBI 2084 TGTACA T^GTACA
SspI 428 AATATT AAT^ATT
SwaI 3832 ATTTAAAT ATTT^AAAT
TfiI 3421 GAATC G^A[AT]TC
XcmI 530 CCAAGGCAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1775 CCCGGG C^CCGGG
XmaI 1775 CCCGGG C^CCGGG
XmnI 240 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcyI 1015 GGCGTC G[GA]^CG[CT]C
1874 GGCGTC
AspI 2659 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3500 GACTCTGTC
BsaBI 1156 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3654 GATCCAAATC
BsaHI 1015 GGCGTC G[GA]^CG[CT]C
1874 GGCGTC
BsiEI 85 CGGCCG CG[GA][CT]^CG
122 CGGCCG
BspMI 1910 ACCTGC ACCTGC(4/8)
3281 GCAGGT
BsrDI 3674 CATTGC GCAATG(2/0)
2996 GCAATG
DraI 3564 TTTAAA TTT^AAA
3833 TTTAAA
DraIII 992 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1325 CACTGTGTG
EagI 85 CGGCCG C^GGCCG
122 CGGCCG
EclXI 85 CGGCCG C^GGCCG
122 CGGCCG
Eco52I 85 CGGCCG C^GGCCG
122 CGGCCG
Hin1I 1015 GGCGTC G[GA]^CG[CT]C
1874 GGCGTC
MamI 1156 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3654 GATCCAAATC
PflFI 2659 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3500 GACTCTGTC
PshAI 471 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2960 GACCCAGGTC
SfiI 123 GGCCGTGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2358 GGCCCAGCAGGCC
Tth111I 2659 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3500 GACTCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AvaI AvaII BalI
BamHI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI StuI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII