##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccIII AclI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BbvCI BcgI BciVI BglII BlnI
BsaBI BsaI BseAI BsiWI BsmBI
BspDI BspEI BspHI BsrBI BssHII
ClaI CpoI DrdI Eam1105I Eco47III
FseI FspI KpnI KspI MamI
MfeI MroI MunI NaeI NgoMIV
NheI NotI PacI PaeR7I PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SfiI
SgrAI SmaI SnaBI SphI Sse8387I
StuI SwaI XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccI 3508 GTATAC GT^[AC][GT]AC
AflII 3739 CTTAAG C^TTAAG
AgeI 1902 ACCGGT A^CCGGT
AspHI 1584 GAGCTC G[AT]GC[AT]^C
AspI 2791 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2354 CCCGAG C^[CT]CG[GA]G
BamHI 374 GGATCC G^GATCC
BbeI 849 GGCGCC GGCGC^C
BbrPI 3003 CACGTG CAC^GTG
BclI 3231 TGATCA T^GATCA
BfrI 3739 CTTAAG C^TTAAG
BglI 3016 GCCAGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2015 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2015 GCTTAGC GC^T[ACGT]AGC
BsiEI 812 CGGCCG CG[GA][CT]^CG
BsiHKAI 1584 GAGCTC G[AT]GC[AT]^C
BsoBI 2354 CCCGAG C^[CT]CG[GA]G
BsrFI 1902 ACCGGT [GA]^CCGG[CT]
BssSI 1261 CTCGTG CACGAG(-5/-1)
Bst1107I 3508 GTATAC GTA^TAC
BstBI 11 TTCGAA TT^CGAA
BstXI 3137 CCAAATACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3508 GTATAC GTA^TAC
CelII 2015 GCTTAGC GC^T[ACGT]AGC
Cfr10I 1902 ACCGGT [GA]^CCGG[CT]
EagI 812 CGGCCG C^GGCCG
EclXI 812 CGGCCG C^GGCCG
Eco52I 812 CGGCCG C^GGCCG
EcoRI 2403 GAATTC G^AATTC
EcoT22I 1038 ATGCAT ATGCA^T
FbaI 3231 TGATCA T^GATCA
HaeII 849 GGCGCC [GA]GCGC^[CT]
KasI 849 GGCGCC G^GCGCC
MluI 3060 ACGCGT A^CGCGT
NarI 849 GGCGCC GG^CGCC
NdeI 2582 CATATG CA^TATG
NruI 2909 TCGCGA TCG^CGA
NsiI 1038 ATGCAT ATGCA^T
NspV 11 TTCGAA TT^CGAA
PflFI 2791 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2935 CCATTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1902 ACCGGT A^CCGGT
PmaCI 3003 CACGTG CAC^GTG
PmlI 3003 CACGTG CAC^GTG
PvuII 1843 CAGCTG CAG^CTG
SacI 1584 GAGCTC GAGCT^C
SexAI 1706 ACCTGGT A^CC[AT]GGT
SfoI 849 GGCGCC GGC^GCC
SfuI 11 TTCGAA TT^CGAA
SpeI 3355 ACTAGT A^CTAGT
Tth111I 2791 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2935 CCATTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AccII 2910 CGCG CG^CG
3061 CGCG
AcyI 638 GACGCC G[GA]^CG[CT]C
849 GGCGCC
AocI 1102 CCTCAGG CC^T[ACGT]AGG
2926 CCTGAGG
ApaI 153 GGGCCC GGGCC^C
1743 GGGCCC
Asp700I 2033 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2399 GAATGAATTC
BalI 1265 TGGCCA TGG^CCA
2145 TGGCCA
BanI 332 GGCACC G^G[CT][GA]CC
849 GGCGCC
BcnI 311 CCGGG CC^[GC]GG
2672 CCGGG
BsaAI 1494 CACGTA [CT]AC^GT[GA]
3003 CACGTG
BsaHI 638 GACGCC G[GA]^CG[CT]C
849 GGCGCC
BsaWI 1902 ACCGGT [AT]^CCGG[AT]
3065 TCCGGT
Bsp1407I 1090 TGTACA T^GTACA
3561 TGTACA
BsrGI 1090 TGTACA T^GTACA
3561 TGTACA
BstAPI 275 GCAGCGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
927 GCAGTCAGTGC
BstEII 1624 GGTAACC G^GT[ACGT]ACC
3798 GGTTACC
BstPI 1624 GGTAACC G^GT[ACGT]ACC
3798 GGTTACC
BstUI 2910 CGCG CG^CG
3061 CGCG
Bsu36I 1102 CCTCAGG CC^T[ACGT]AGG
2926 CCTGAGG
DraI 3521 TTTAAA TTT^AAA
3894 TTTAAA
Eco81I 1102 CCTCAGG CC^T[ACGT]AGG
2926 CCTGAGG
EcoO65I 1624 GGTAACC G^GT[ACGT]ACC
3798 GGTTACC
EcoRV 721 GATATC GAT^ATC
1957 GATATC
FauI 755 CCCGC CCCGC(4/6)
2483 CCCGC
HgaI 638 GACGC GACGC(5/10)
3062 GCGTC
Hin1I 638 GACGCC G[GA]^CG[CT]C
849 GGCGCC
HincII 3296 GTTAAC GT[CT]^[GA]AC
3530 GTTAAC
HindII 3296 GTTAAC GT[CT]^[GA]AC
3530 GTTAAC
HpaI 3296 GTTAAC GTT^AAC
3530 GTTAAC
MluNI 1265 TGGCCA TGG^CCA
2145 TGGCCA
MscI 1265 TGGCCA TGG^CCA
2145 TGGCCA
MspA1I 815 CCGCTG C[AC]G^C[GT]G
1843 CAGCTG
MvnI 2910 CGCG CG^CG
3061 CGCG
NciI 311 CCGGG CC^[GC]GG
2672 CCGGG
NcoI 149 CCATGG C^CATGG
157 CCATGG
PpuMI 824 GGGTCCT [GA]G^G[AT]CC[CT]
2358 AGGACCC
SapI 1075 GAAGAGC GCTCTTC(1/4)
1581 GAAGAGC
ScaI 1298 AGTACT AGT^ACT
1970 AGTACT
SspBI 1090 TGTACA T^GTACA
3561 TGTACA
XcmI 197 CCACAGATGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1151 CCAACAAAATCGTGG
XmnI 2033 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2399 GAATGAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AvaII BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspLU11I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII