##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AclI AflIII AgeI AscI AseI
AsnI AviII AvrII BamHI BbrPI
BciVI BglII BlnI BsaAI BsaBI
BsiWI BspDI BspLU11I Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
EcoO65I EcoRV FseI FspI HindIII
HpaI KspI MamI MfeI MluI
MunI NheI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI Sse8387I SspI SwaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 1197 GACGTC GACGT^C
Acc65I 3190 GGTACC G^GTACC
AflII 1816 CTTAAG C^TTAAG
AhdI 3007 GACACGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3840 GTGCAC G^TGCAC
Aor51HI 752 AGCGCT AGC^GCT
ApaI 722 GGGCCC GGGCC^C
ApaLI 3840 GTGCAC G^TGCAC
Asp700I 1635 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3190 GGTACC G^GTACC
AspEI 3007 GACACGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BclI 2781 TGATCA T^GATCA
BfrI 1816 CTTAAG C^TTAAG
Bsp1407I 1367 TGTACA T^GTACA
BsrDI 30 GCAATG GCAATG(2/0)
BsrGI 1367 TGTACA T^GTACA
BssSI 297 CACGAG CACGAG(-5/-1)
DraIII 229 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1738 GACGTGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2248 CGGCCG C^GGCCG
Eam1105I 3007 GACACGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2248 CGGCCG C^GGCCG
Eco47III 752 AGCGCT AGC^GCT
Eco52I 2248 CGGCCG C^GGCCG
EcoRI 3170 GAATTC G^AATTC
EcoT22I 2339 ATGCAT ATGCA^T
FbaI 2781 TGATCA T^GATCA
HincII 1262 GTCGAC GT[CT]^[GA]AC
HindII 1262 GTCGAC GT[CT]^[GA]AC
KpnI 3190 GGTACC GGTAC^C
NdeI 1958 CATATG CA^TATG
NsiI 2339 ATGCAT ATGCA^T
PaeR7I 2320 CTCGAG C^TCGAG
SalI 1262 GTCGAC G^TCGAC
SapI 1557 GCTCTTC GCTCTTC(1/4)
SpeI 507 ACTAGT A^CTAGT
SphI 3098 GCATGC GCATG^C
SspBI 1367 TGTACA T^GTACA
StuI 2303 AGGCCT AGG^CCT
XbaI 2769 TCTAGA T^CTAGA
XhoI 2320 CTCGAG C^TCGAG
XmnI 1635 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccIII 2092 TCCGGA T^CCGGA
3254 TCCGGA
AspI 1972 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3881 GACCGAGTC
BcgI 3581 GCAGACCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4234 GCAGGAGACTCG
BlpI 1927 GCTCAGC GC^T[ACGT]AGC
2422 GCTGAGC
Bpu1102I 1927 GCTCAGC GC^T[ACGT]AGC
2422 GCTGAGC
BsaWI 2092 TCCGGA [AT]^CCGG[AT]
3254 TCCGGA
BseAI 2092 TCCGGA T^CCGGA
3254 TCCGGA
BsiEI 2244 CGGTCG CG[GA][CT]^CG
2248 CGGCCG
BsmBI 4157 CGTCTC CGTCTC(1/5)
1195 GAGACG
BspEI 2092 TCCGGA T^CCGGA
3254 TCCGGA
BssHII 919 GCGCGC G^CGCGC
921 GCGCGC
BstAPI 1374 GCACATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3098 GCATGCCATGC
CelII 1927 GCTCAGC GC^T[ACGT]AGC
2422 GCTGAGC
DraI 3983 TTTAAA TTT^AAA
4029 TTTAAA
EarI 1558 CTCTTC CTCTTC(1/4)
3544 GAAGAG
Ksp632I 1558 CTCTTC CTCTTC(1/4)
3544 GAAGAG
MroI 2092 TCCGGA T^CCGGA
3254 TCCGGA
PflFI 1972 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3881 GACCGAGTC
PflMI 3195 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3914 CCAACTCTTGG
PshAI 1137 GACAGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3085 GACCACAGTC
SacI 300 GAGCTC GAGCT^C
3144 GAGCTC
Tth111I 1972 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3881 GACCGAGTC
Van91I 3195 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3914 CCAACTCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspHI BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmaCI XmaI