##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII AccI AclI AflII AseI
AsnI AviII AvrII BbrPI BfrI
BlnI BsiWI BsmBI BsmI BspDI
BspLU11I Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI EcoO65I
EcoRV EcoT22I FseI FspI HincII
HindII HindIII HpaI MfeI MunI
NdeI NotI NruI NsiI NspV
PacI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SalI
SbfI SfuI SnaBI SpeI Sse8387I
SwaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 1073 GGTACC G^GTACC
AgeI 2792 ACCGGT A^CCGGT
AscI 155 GGCGCGCC GG^CGCGCC
Asp718I 1073 GGTACC G^GTACC
BbeI 180 GGCGCC GGCGC^C
BciVI 1481 GTATCC GTATCC(6/5)
BglII 3382 AGATCT A^GATCT
BsaAI 462 CACGTA [CT]AC^GT[GA]
BsaBI 2517 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrDI 3297 CATTGC GCAATG(2/0)
BstAPI 2562 GCAGGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EagI 207 CGGCCG C^GGCCG
EclXI 207 CGGCCG C^GGCCG
Eco52I 207 CGGCCG C^GGCCG
EcoRI 4219 GAATTC G^AATTC
KasI 180 GGCGCC G^GCGCC
KpnI 1073 GGTACC GGTAC^C
KspI 237 CCGCGG CCGC^GG
MamI 2517 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 180 GGCGCC GG^CGCC
NcoI 1002 CCATGG C^CATGG
NheI 3735 GCTAGC G^CTAGC
PinAI 2792 ACCGGT A^CCGGT
SacII 237 CCGCGG CCGC^GG
SapI 3988 GCTCTTC GCTCTTC(1/4)
ScaI 2597 AGTACT AGT^ACT
SexAI 557 ACCTGGT A^CC[AT]GGT
SfiI 2208 GGCCCTGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 180 GGCGCC GGC^GCC
SgrAI 23 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 868 CCCGGG CCC^GGG
SphI 1811 GCATGC GCATG^C
SspI 4199 AATATT AAT^ATT
XbaI 4322 TCTAGA T^CTAGA
XmaCI 868 CCCGGG C^CCGGG
XmaI 868 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 83 TCCGGA T^CCGGA
902 TCCGGA
AcsI 2392 GAATTT [GA]^AATT[CT]
4219 GAATTC
Alw44I 3108 GTGCAC G^TGCAC
3789 GTGCAC
Aor51HI 2463 AGCGCT AGC^GCT
2606 AGCGCT
ApaLI 3108 GTGCAC G^TGCAC
3789 GTGCAC
ApoI 2392 GAATTT [GA]^AATT[CT]
4219 GAATTC
Asp700I 2950 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4169 GAAGTTGTTC
AspI 2470 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4315 GACAAGGTC
BamHI 3909 GGATCC G^GATCC
4359 GGATCC
BbsI 1204 GTCTTC GAAGAC(2/6)
2104 GTCTTC
BbvCI 2262 CCTCAGC CCTCAGC(-5/-2)
3739 GCTGAGG
BclI 458 TGATCA T^GATCA
4416 TGATCA
BpuAI 1204 GTCTTC GAAGAC(2/6)
2104 GTCTTC
BseAI 83 TCCGGA T^CCGGA
902 TCCGGA
BsiEI 207 CGGCCG CG[GA][CT]^CG
1486 CGACCG
Bsp1407I 1037 TGTACA T^GTACA
4532 TGTACA
BspEI 83 TCCGGA T^CCGGA
902 TCCGGA
BsrGI 1037 TGTACA T^GTACA
4532 TGTACA
BssSI 2356 CTCGTG CACGAG(-5/-1)
4097 CTCGTG
DraIII 2437 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
3102 CACAAGGTG
DrdI 1126 GACGCCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1738 GACGATCTGGTC
Eco47III 2463 AGCGCT AGC^GCT
2606 AGCGCT
FbaI 458 TGATCA T^GATCA
4416 TGATCA
MluI 4 ACGCGT A^CGCGT
2633 ACGCGT
MroI 83 TCCGGA T^CCGGA
902 TCCGGA
NspI 1020 GCATGT [GA]CATG^[CT]
1811 GCATGC
PaeR7I 824 CTCGAG C^TCGAG
2818 CTCGAG
PflFI 2470 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4315 GACAAGGTC
SacI 1680 GAGCTC GAGCT^C
2353 GAGCTC
SspBI 1037 TGTACA T^GTACA
4532 TGTACA
Tth111I 2470 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4315 GACAAGGTC
XhoI 824 CTCGAG C^TCGAG
2818 CTCGAG
XmnI 2950 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4169 GAAGTTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AhdI AluI AlwI AlwNI AocI
ApaI AspEI AspHI AvaI AvaII
BalI BanI BanII BbvI BcgI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BspMI BsrBI BsrFI BsrI
BssHII BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI Eam1105I EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII