##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
Acc65I AccII AccIII AflII AhdI
AscI Asp718I AspEI AviII AvrII
BalI BamHI BbeI BbrPI BcgI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BsrDI BsrGI
BssHII BstEII BstPI BstUI CelII
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HgaI
HindIII HpaI KasI KpnI KspI
MamI MluI MluNI MroI MscI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PmaCI PmlI PshAI PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI StuI XcmI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 2210 GACGTC GACGT^C
AclI 2685 AACGTT AA^CGTT
AcyI 2210 GACGTC G[GA]^CG[CT]C
AgeI 3206 ACCGGT A^CCGGT
Alw44I 1154 GTGCAC G^TGCAC
Aor51HI 2149 AGCGCT AGC^GCT
ApaI 2469 GGGCCC GGGCC^C
ApaLI 1154 GTGCAC G^TGCAC
AseI 2009 ATTAAT AT^TAAT
AsnI 2009 ATTAAT AT^TAAT
BanI 2494 GGCACC G^G[CT][GA]CC
BciVI 2375 GTATCC GTATCC(6/5)
BclI 1790 TGATCA T^GATCA
BcnI 1679 CCCGG CC^[GC]GG
BglII 2355 AGATCT A^GATCT
BsaHI 2210 GACGTC G[GA]^CG[CT]C
BsaWI 3206 ACCGGT [AT]^CCGG[AT]
BsmBI 1734 CGTCTC CGTCTC(1/5)
BsmI 3286 GCATTC GAATGC(1/-1)
BspHI 3910 TCATGA T^CATGA
BsrBI 429 GAGCGG CCGCTC(-3/-3)
BssSI 2562 CACGAG CACGAG(-5/-1)
BstAPI 311 GCAGCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 958 TTCGAA TT^CGAA
EaeI 322 TGGCCG [CT]^GGCC[GA]
Eco47III 2149 AGCGCT AGC^GCT
EcoNI 2637 CCTACCTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1790 TGATCA T^GATCA
HaeII 2149 AGCGCT [GA]GCGC^[CT]
Hin1I 2210 GACGTC G[GA]^CG[CT]C
MfeI 1743 CAATTG C^AATTG
MunI 1743 CAATTG C^AATTG
NciI 1679 CCCGG CC^[GC]GG
NspV 958 TTCGAA TT^CGAA
PinAI 3206 ACCGGT A^CCGGT
PmeI 3032 GTTTAAAC GTTT^AAAC
PpuMI 184 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 2009 ATTAAT AT^TAAT
Psp1406I 2685 AACGTT AA^CGTT
RcaI 3910 TCATGA T^CATGA
SapI 3774 GCTCTTC GCTCTTC(1/4)
SfuI 958 TTCGAA TT^CGAA
SphI 301 GCATGC GCATG^C
SspI 3556 AATATT AAT^ATT
SwaI 1168 ATTTAAAT ATTT^AAAT
XbaI 2366 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 3534 ACATGT A^C[GA][CT]GT
4023 ACATGT
AlwI 101 GATCC GGATC(4/5)
617 GGATC
AocI 132 CCTGAGG CC^T[ACGT]AGG
2641 CCTTAGG
AspI 2840 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3377 GACTCAGTC
AvaI 3226 CTCGAG C^[CT]CG[GA]G
3367 CTCGAG
BbvCI 632 GCTGAGG CCTCAGC(-5/-2)
3448 GCTGAGG
BsaI 849 GAGACC GGTCTC(1/5)
3025 GGTCTC
BsoBI 3226 CTCGAG C^[CT]CG[GA]G
3367 CTCGAG
BspLU11I 3534 ACATGT A^CATGT
4023 ACATGT
Bst1107I 348 GTATAC GTA^TAC
1503 GTATAC
BstXI 412 CCAACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2128 CCAAAGAGTTGG
BstYI 617 GGATCT [GA]^GATC[CT]
2355 AGATCT
BstZ17I 348 GTATAC GTA^TAC
1503 GTATAC
Bsu36I 132 CCTGAGG CC^T[ACGT]AGG
2641 CCTTAGG
Eco81I 132 CCTGAGG CC^T[ACGT]AGG
2641 CCTTAGG
EcoT14I 2421 CCATGG C^C[AT][AT]GG
2902 CCATGG
HincII 1587 GTTGAC GT[CT]^[GA]AC
2213 GTCAAC
HindII 1587 GTTGAC GT[CT]^[GA]AC
2213 GTCAAC
MflI 617 GGATCT [GA]^GATC[CT]
2355 AGATCT
NcoI 2421 CCATGG C^CATGG
2902 CCATGG
PaeR7I 3226 CTCGAG C^TCGAG
3367 CTCGAG
PflFI 2840 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3377 GACTCAGTC
StyI 2421 CCATGG C^C[AT][AT]GG
2902 CCATGG
Tth111I 2840 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3377 GACTCAGTC
XhoI 3226 CTCGAG C^TCGAG
3367 CTCGAG
XhoII 617 GGATCT [GA]^GATC[CT]
2355 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwNI ApoI Asp700I AspHI AvaII
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XmnI