##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AclI AflIII AgeI
AhdI AocI Aor51HI AscI Asp718I
AspEI BclI BglII BsaBI BsiEI
BsiWI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrDI BsrGI Bst1107I BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV EcoT22I
FbaI FseI HindIII KpnI KspI
MamI MfeI MluI MunI NdeI
NheI NotI NruI NsiI PacI
PinAI PmeI PshAI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SexAI SfiI SgrAI SnaBI SpeI
SspBI SspI SwaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 3089 GTCTAC GT^[AC][GT]AC
AcyI 93 GGCGCC G[GA]^CG[CT]C
AflII 936 CTTAAG C^TTAAG
Alw44I 1880 GTGCAC G^TGCAC
ApaI 593 GGGCCC GGGCC^C
ApaLI 1880 GTGCAC G^TGCAC
AseI 3012 ATTAAT AT^TAAT
AsnI 3012 ATTAAT AT^TAAT
AspI 3642 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3571 CCTAGG C^CTAGG
BalI 3457 TGGCCA TGG^CCA
BamHI 1622 GGATCC G^GATCC
BbeI 93 GGCGCC GGCGC^C
BbrPI 129 CACGTG CAC^GTG
BfrI 936 CTTAAG C^TTAAG
BlnI 3571 CCTAGG C^CTAGG
BlpI 653 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 653 GCTGAGC GC^T[ACGT]AGC
BsaAI 129 CACGTG [CT]AC^GT[GA]
BsaHI 93 GGCGCC G[GA]^CG[CT]C
BsmBI 408 GAGACG CGTCTC(1/5)
BssHII 2489 GCGCGC G^CGCGC
BstBI 3601 TTCGAA TT^CGAA
CelII 653 GCTGAGC GC^T[ACGT]AGC
DraI 3256 TTTAAA TTT^AAA
DraIII 1341 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
Hin1I 93 GGCGCC G[GA]^CG[CT]C
HincII 3347 GTTAAC GT[CT]^[GA]AC
HindII 3347 GTTAAC GT[CT]^[GA]AC
HpaI 3347 GTTAAC GTT^AAC
KasI 93 GGCGCC G^GCGCC
MluNI 3457 TGGCCA TGG^CCA
MscI 3457 TGGCCA TGG^CCA
NarI 93 GGCGCC GG^CGCC
NcoI 2626 CCATGG C^CATGG
NspI 2029 GCATGC [GA]CATG^[CT]
NspV 3601 TTCGAA TT^CGAA
PaeR7I 2001 CTCGAG C^TCGAG
PflFI 3642 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 129 CACGTG CAC^GTG
PmlI 129 CACGTG CAC^GTG
PshBI 3012 ATTAAT AT^TAAT
ScaI 3193 AGTACT AGT^ACT
SfoI 93 GGCGCC GGC^GCC
SfuI 3601 TTCGAA TT^CGAA
SphI 2029 GCATGC GCATG^C
StuI 2140 AGGCCT AGG^CCT
Tth111I 3642 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1805 TCTAGA T^CTAGA
XcmI 469 CCAGGGAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2001 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccIII 832 TCCGGA T^CCGGA
2703 TCCGGA
Asp700I 309 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1248 GAAGACCTTC
AviII 297 TGCGCA TGC^GCA
2026 TGCGCA
BanI 93 GGCGCC G^G[CT][GA]CC
2263 GGCACC
BcgI 142 GCAAGAAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1028 GCAGGAGTGTCG
BciVI 3221 GGATAC GTATCC(6/5)
3235 GGATAC
BseAI 832 TCCGGA T^CCGGA
2703 TCCGGA
BspEI 832 TCCGGA T^CCGGA
2703 TCCGGA
BspMI 1903 ACCTGC ACCTGC(4/8)
2542 GCAGGT
BssSI 528 CACGAG CACGAG(-5/-1)
395 CTCGTG
BstYI 1622 GGATCC [GA]^GATC[CT]
2107 AGATCC
EcoRI 956 GAATTC G^AATTC
1499 GAATTC
FspI 297 TGCGCA TGC^GCA
2026 TGCGCA
MaeII 130 ACGT A^CGT
2932 ACGT
MflI 1622 GGATCC [GA]^GATC[CT]
2107 AGATCC
MroI 832 TCCGGA T^CCGGA
2703 TCCGGA
PflMI 329 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2138 CCAGGCCTTGG
SmaI 2384 CCCGGG CCC^GGG
2888 CCCGGG
Van91I 329 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2138 CCAGGCCTTGG
XhoII 1622 GGATCC [GA]^GATC[CT]
2107 AGATCC
XmaCI 2384 CCCGGG C^CCGGG
2888 CCCGGG
XmaI 2384 CCCGGG C^CCGGG
2888 CCCGGG
XmnI 309 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1248 GAAGACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanII BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SmlI Sse8387I StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I