##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AclI AflII Alw44I ApaLI
AscI AspI AviII AvrII BbsI
BcgI BciVI BfrI BlnI BpuAI
BsaBI BsiEI BspDI BspHI BssHII
BssSI BstBI ClaI CpoI DraIII
DrdI EagI EclXI Eco52I FseI
FspI HindIII MamI MfeI MluI
MunI NaeI NgoMIV NheI NotI
NruI NspV PacI PflFI PmeI
PshAI Psp1406I PvuI RcaI RsrII
SalI SbfI SexAI SfiI SfuI
SgrAI Sse8387I SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 1836 GGTACC G^GTACC
AccIII 3601 TCCGGA T^CCGGA
AgeI 1138 ACCGGT A^CCGGT
AocI 1831 CCTGAGG CC^T[ACGT]AGG
AseI 2236 ATTAAT AT^TAAT
AsnI 2236 ATTAAT AT^TAAT
Asp700I 5574 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1836 GGTACC G^GTACC
BamHI 160 GGATCC G^GATCC
BbrPI 1306 CACGTG CAC^GTG
BclI 3321 TGATCA T^GATCA
BglI 4752 GCCTTAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1905 AGATCT A^GATCT
BsaI 422 GAGACC GGTCTC(1/5)
BseAI 3601 TCCGGA T^CCGGA
BsiWI 1323 CGTACG C^GTACG
BsmBI 2600 GAGACG CGTCTC(1/5)
Bsp1407I 4676 TGTACA T^GTACA
BspEI 3601 TCCGGA T^CCGGA
BsrBI 4442 GAGCGG CCGCTC(-3/-3)
BsrGI 4676 TGTACA T^GTACA
BstEII 4535 GGTTACC G^GT[ACGT]ACC
BstPI 4535 GGTTACC G^GT[ACGT]ACC
Bsu36I 1831 CCTGAGG CC^T[ACGT]AGG
Eco81I 1831 CCTGAGG CC^T[ACGT]AGG
EcoO65I 4535 GGTTACC G^GT[ACGT]ACC
EcoRV 1589 GATATC GAT^ATC
EcoT22I 3372 ATGCAT ATGCA^T
FbaI 3321 TGATCA T^GATCA
HpaI 3985 GTTAAC GTT^AAC
KpnI 1836 GGTACC GGTAC^C
KspI 657 CCGCGG CCGC^GG
MroI 3601 TCCGGA T^CCGGA
NcoI 4732 CCATGG C^CATGG
NdeI 3375 CATATG CA^TATG
NsiI 3372 ATGCAT ATGCA^T
PinAI 1138 ACCGGT A^CCGGT
PmaCI 1306 CACGTG CAC^GTG
PmlI 1306 CACGTG CAC^GTG
PpuMI 2027 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 2236 ATTAAT AT^TAAT
SacII 657 CCGCGG CCGC^GG
SapI 1764 GCTCTTC GCTCTTC(1/4)
SnaBI 4856 TACGTA TAC^GTA
SpeI 293 ACTAGT A^CTAGT
SspBI 4676 TGTACA T^GTACA
SspI 1127 AATATT AAT^ATT
StuI 390 AGGCCT AGG^CCT
XbaI 104 TCTAGA T^CTAGA
XmnI 5574 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
AccII 658 CGCG CG^CG
862 CGCG
AcyI 546 GGCGCC G[GA]^CG[CT]C
722 GGCGCC
AflIII 1184 ACATGT A^C[GA][CT]GT
4673 ACATGT
AhdI 2978 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3392 GACCAGAAGTC
AlwNI 3458 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
4946 CAGGGTCTG
Aor51HI 577 AGCGCT AGC^GCT
4709 AGCGCT
ApaI 1223 GGGCCC GGGCC^C
1362 GGGCCC
AspEI 2978 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3392 GACCAGAAGTC
BbeI 546 GGCGCC GGCGC^C
722 GGCGCC
BlpI 3722 GCTAAGC GC^T[ACGT]AGC
4439 GCTGAGC
Bpu1102I 3722 GCTAAGC GC^T[ACGT]AGC
4439 GCTGAGC
BsaHI 546 GGCGCC G[GA]^CG[CT]C
722 GGCGCC
BsaWI 1138 ACCGGT [AT]^CCGG[AT]
3601 TCCGGA
BsgI 1345 CTGCAC GTGCAG(16/14)
4502 CTGCAC
BspLU11I 1184 ACATGT A^CATGT
4673 ACATGT
BsrFI 1138 ACCGGT [GA]^CCGG[CT]
1996 ACCGGC
Bst1107I 3860 GTATAC GTA^TAC
4859 GTATAC
BstAPI 766 GCACGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1436 GCAAGGTCTGC
BstUI 658 CGCG CG^CG
862 CGCG
BstYI 160 GGATCC [GA]^GATC[CT]
1905 AGATCT
BstZ17I 3860 GTATAC GTA^TAC
4859 GTATAC
CelII 3722 GCTAAGC GC^T[ACGT]AGC
4439 GCTGAGC
Cfr10I 1138 ACCGGT [GA]^CCGG[CT]
1996 ACCGGC
DsaI 657 CCGCGG C^C[GA][CT]GG
4732 CCATGG
Eam1105I 2978 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3392 GACCAGAAGTC
Eco47III 577 AGCGCT AGC^GCT
4709 AGCGCT
Hin1I 546 GGCGCC G[GA]^CG[CT]C
722 GGCGCC
KasI 546 GGCGCC G^GCGCC
722 GGCGCC
MflI 160 GGATCC [GA]^GATC[CT]
1905 AGATCT
MvnI 658 CGCG CG^CG
862 CGCG
NarI 546 GGCGCC GG^CGCC
722 GGCGCC
PaeR7I 2827 CTCGAG C^TCGAG
3814 CTCGAG
PflMI 3457 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5514 CCATTGCCTGG
SacI 110 GAGCTC GAGCT^C
3518 GAGCTC
SfoI 546 GGCGCC GGC^GCC
722 GGCGCC
SmaI 1456 CCCGGG CCC^GGG
4549 CCCGGG
SphI 4162 GCATGC GCATG^C
4387 GCATGC
Van91I 3457 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5514 CCATTGCCTGG
XhoI 2827 CTCGAG C^TCGAG
3814 CTCGAG
XhoII 160 GGATCC [GA]^GATC[CT]
1905 AGATCT
XmaCI 1456 CCCGGG C^CCGGG
4549 CCCGGG
XmaI 1456 CCCGGG C^CCGGG
4549 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BcnI BfaI BmyI BpmI BsaAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoNI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI