##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AflIII AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BspDI BspEI BspHI BspLU11I
BspMI BsrBI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HgaI Hin1I HincII HindII
HpaI KasI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MseI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tru9I Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 227 GGTACC G^GTACC
AccII 1346 CGCG CG^CG
AcsI 327 GAATTC [GA]^AATT[CT]
Alw44I 220 GTGCAC G^TGCAC
ApaLI 220 GTGCAC G^TGCAC
ApoI 327 GAATTC [GA]^AATT[CT]
Asp718I 227 GGTACC G^GTACC
BcgI 289 CGAGCCCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1376 CTAG C^TAG
BsmI 511 GCATTC GAATGC(1/-1)
Bsp1407I 953 TGTACA T^GTACA
BsrDI 854 GCAATG GCAATG(2/0)
BsrFI 1075 ACCGGC [GA]^CCGG[CT]
BsrGI 953 TGTACA T^GTACA
BssHII 1345 GCGCGC G^CGCGC
BstUI 1346 CGCG CG^CG
BstXI 950 CCATGTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1075 ACCGGC [GA]^CCGG[CT]
EarI 787 CTCTTC CTCTTC(1/4)
EcoRI 327 GAATTC G^AATTC
HaeII 1218 GGCGCT [GA]GCGC^[CT]
HindIII 599 AAGCTT A^AGCTT
KpnI 227 GGTACC GGTAC^C
Ksp632I 787 CTCTTC CTCTTC(1/4)
MaeI 1376 CTAG C^TAG
MvnI 1346 CGCG CG^CG
NspI 721 GCATGT [GA]CATG^[CT]
PaeR7I 287 CTCGAG C^TCGAG
PflMI 1281 CCACATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1113 CAGCTG CAG^CTG
SacI 784 GAGCTC GAGCT^C
SapI 786 GCTCTTC GCTCTTC(1/4)
SspBI 953 TGTACA T^GTACA
Van91I 1281 CCACATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 287 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BanII 290 GAGCCC G[GA]GC[CT]^C
784 GAGCTC
BbsI 1267 GAAGAC GAAGAC(2/6)
148 GTCTTC
BglII 136 AGATCT A^GATCT
656 AGATCT
BpuAI 1267 GAAGAC GAAGAC(2/6)
148 GTCTTC
BsaI 43 GAGACC GGTCTC(1/5)
320 GAGACC
DraII 452 AGGTCCT [GA]G^G[ACGT]CC[CT]
1061 GGGACCC
EcoO109I 452 AGGTCCT [GA]G^G[ACGT]CC[CT]
1061 GGGACCC
EcoT14I 25 CCTTGG C^C[AT][AT]GG
532 CCAAGG
HphI 2 GGTGA GGTGA(8/7)
1212 GGTGA
MslI 951 CATGTACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
985 CACGCCAATG
PpuMI 452 AGGTCCT [GA]G^G[AT]CC[CT]
1061 GGGACCC
PstI 793 CTGCAG CTGCA^G
944 CTGCAG
SmaI 179 CCCGGG CCC^GGG
1208 CCCGGG
SmlI 287 CTCGAG C^T[CT][GA]AG
445 CTCAAG
StyI 25 CCTTGG C^C[AT][AT]GG
532 CCAAGG
TaqI 288 TCGA T^CGA
782 TCGA
Tsp45I 1072 GTGAC ^GT[GC]AC
1371 GTGAC
TthHB8I 288 TCGA T^CGA
782 TCGA
XcmI 160 CCAGCCCCCTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
232 CCAGTCCTGGCCTGG
XmaCI 179 CCCGGG C^CCGGG
1208 CCCGGG
XmaI 179 CCCGGG C^CCGGG
1208 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BanI BbvI
BcnI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TfiI TseI Tsp509I
TspRI XhoII