##################################
# NO CUT ENZYMES ( 32 enzymes) #
##################################
BsiWI BspDI Bst1107I BstBI BstZ17I
ClaI CpoI EagI EclXI Eco52I
EcoRI FseI HindIII MfeI MluI
MunI NdeI NheI NotI NspV
PacI PmeI PshAI RsrII SalI
SapI SexAI SfiI SfuI SgrAI
SpeI SwaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 2973 GACGTC GACGT^C
Acc65I 3161 GGTACC G^GTACC
AccIII 2405 TCCGGA T^CCGGA
AclI 6128 AACGTT AA^CGTT
AgeI 5629 ACCGGT A^CCGGT
AocI 4804 CCTTAGG CC^T[ACGT]AGG
AscI 2703 GGCGCGCC GG^CGCGCC
Asp718I 3161 GGTACC G^GTACC
AviII 2918 TGCGCA TGC^GCA
AvrII 3870 CCTAGG C^CTAGG
BamHI 1656 GGATCC G^GATCC
BbrPI 1254 CACGTG CAC^GTG
BbvCI 3698 CCTCAGC CCTCAGC(-5/-2)
BcgI 352 GCATGGGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4619 GTATCC GTATCC(6/5)
BglII 98 AGATCT A^GATCT
BlnI 3870 CCTAGG C^CTAGG
BsaBI 1377 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2405 TCCGGA T^CCGGA
BsiEI 3067 CGATCG CG[GA][CT]^CG
BspEI 2405 TCCGGA T^CCGGA
BsrBI 284 GAGCGG CCGCTC(-3/-3)
Bsu36I 4804 CCTTAGG CC^T[ACGT]AGG
DraIII 3781 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 4804 CCTTAGG CC^T[ACGT]AGG
EcoRV 221 GATATC GAT^ATC
FspI 2918 TGCGCA TGC^GCA
HpaI 6503 GTTAAC GTT^AAC
KpnI 3161 GGTACC GGTAC^C
MamI 1377 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2405 TCCGGA T^CCGGA
NruI 3064 TCGCGA TCG^CGA
PaeR7I 2628 CTCGAG C^TCGAG
PinAI 5629 ACCGGT A^CCGGT
PmaCI 1254 CACGTG CAC^GTG
PmlI 1254 CACGTG CAC^GTG
Psp1406I 6128 AACGTT AA^CGTT
PvuI 3067 CGATCG CGAT^CG
SbfI 5611 CCTGCAGG CCTGCA^GG
SnaBI 4008 TACGTA TAC^GTA
Sse8387I 5611 CCTGCAGG CCTGCA^GG
SspI 4071 AATATT AAT^ATT
XbaI 5508 TCTAGA T^CTAGA
XhoI 2628 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AflII 39 CTTAAG C^TTAAG
5277 CTTAAG
Aor51HI 2243 AGCGCT AGC^GCT
2276 AGCGCT
ApaI 317 GGGCCC GGGCC^C
4882 GGGCCC
Asp700I 4233 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5574 GAAGGGATTC
BbeI 2899 GGCGCC GGCGC^C
3106 GGCGCC
BfrI 39 CTTAAG C^TTAAG
5277 CTTAAG
BglI 3098 GCCGCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3595 GCCAGCCCGGC
BsaAI 1254 CACGTG [CT]AC^GT[GA]
4008 TACGTA
BsmBI 3138 CGTCTC CGTCTC(1/5)
1396 GAGACG
BspLU11I 4989 ACATGT A^CATGT
5867 ACATGT
BssHII 2704 GCGCGC G^CGCGC
2785 GCGCGC
BstAPI 378 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3072 GCAGATCCTGC
DraI 4262 TTTAAA TTT^AAA
6227 TTTAAA
EarI 605 GAAGAG CTCTTC(1/4)
4033 GAAGAG
Eco47III 2243 AGCGCT AGC^GCT
2276 AGCGCT
KasI 2899 GGCGCC G^GCGCC
3106 GGCGCC
Ksp632I 605 GAAGAG CTCTTC(1/4)
4033 GAAGAG
KspI 2346 CCGCGG CCGC^GG
2566 CCGCGG
NaeI 2308 GCCGGC GCC^GGC
3898 GCCGGC
NarI 2899 GGCGCC GG^CGCC
3106 GGCGCC
NgoMIV 2308 GCCGGC G^CCGGC
3898 GCCGGC
PflMI 2124 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2192 CCAAAATGTGG
SacII 2346 CCGCGG CCGC^GG
2566 CCGCGG
ScaI 545 AGTACT AGT^ACT
3475 AGTACT
SfoI 2899 GGCGCC GGC^GCC
3106 GGCGCC
SmaI 313 CCCGGG CCC^GGG
3577 CCCGGG
SphI 2378 GCATGC GCATG^C
2768 GCATGC
Van91I 2124 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2192 CCAAAATGTGG
XmaCI 313 CCCGGG C^CCGGG
3577 CCCGGG
XmaI 313 CCCGGG C^CCGGG
3577 CCCGGG
XmnI 4233 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5574 GAAGGGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 151 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AhdI AluI Alw44I
AlwI AlwNI ApaLI ApoI AseI
AsnI AspEI AspHI AspI AvaI
AvaII BalI BanI BanII BbsI
BbvI BclI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspHI
BspMI BsrDI BsrFI BsrGI BsrI
BssKI BssSI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
Eam1105I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I EcoT22I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NsiI NspI PflFI PleI PpuMI
PshBI PstI PvuII RcaI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XcmI
XhoII