##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AccIII AclI AgeI Alw44I ApaLI
AscI AseI AsnI AviII AvrII
BbrPI BcgI BlnI BsaBI BseAI
BsiWI Bsp1407I BspDI BspEI BspHI
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DrdI EagI EclXI
Eco52I EcoRV FseI FspI HpaI
MamI MroI NaeI NgoMIV NheI
NotI NruI NspV PacI PinAI
PmaCI PmlI PshBI Psp1406I PvuI
RcaI RsrII SbfI ScaI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AatII 4190 GACGTC GACGT^C
Acc65I 4860 GGTACC G^GTACC
Aor51HI 277 AGCGCT AGC^GCT
Asp718I 4860 GGTACC G^GTACC
AspI 893 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1987 GGATCC G^GATCC
BciVI 4197 GTATCC GTATCC(6/5)
BclI 3533 TGATCA T^GATCA
BsaAI 1704 CACGTA [CT]AC^GT[GA]
BsiEI 3288 CGACCG CG[GA][CT]^CG
BsmBI 2868 CGTCTC CGTCTC(1/5)
BsrFI 1403 ACCGGC [GA]^CCGG[CT]
BstAPI 3013 GCATAGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 1403 ACCGGC [GA]^CCGG[CT]
Eco47III 277 AGCGCT AGC^GCT
EcoRI 50 GAATTC G^AATTC
FbaI 3533 TGATCA T^GATCA
HincII 899 GTCGAC GT[CT]^[GA]AC
HindII 899 GTCGAC GT[CT]^[GA]AC
KpnI 4860 GGTACC GGTAC^C
MfeI 4809 CAATTG C^AATTG
MluI 207 ACGCGT A^CGCGT
MunI 4809 CAATTG C^AATTG
PflFI 893 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmeI 3760 GTTTAAAC GTTT^AAAC
SalI 899 GTCGAC G^TCGAC
SapI 3935 GAAGAGC GCTCTTC(1/4)
SexAI 5169 ACCAGGT A^CC[AT]GGT
SfiI 2983 GGCCTCGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tth111I 893 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4376 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflII 1912 CTTAAG C^TTAAG
5419 CTTAAG
AhdI 1179 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5587 GACCTCGTGTC
AspEI 1179 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5587 GACCTCGTGTC
BbeI 1822 GGCGCC GGCGC^C
5652 GGCGCC
BbsI 196 GAAGAC GAAGAC(2/6)
890 GAAGAC
BbvCI 2975 GCTGAGG CCTCAGC(-5/-2)
5247 GCTGAGG
BfrI 1912 CTTAAG C^TTAAG
5419 CTTAAG
BlpI 2282 GCTCAGC GC^T[ACGT]AGC
4995 GCTGAGC
Bpu1102I 2282 GCTCAGC GC^T[ACGT]AGC
4995 GCTGAGC
BpuAI 196 GAAGAC GAAGAC(2/6)
890 GAAGAC
BsgI 2358 CTGCAC GTGCAG(16/14)
5646 GTGCAG
BsrDI 1939 CATTGC GCAATG(2/0)
1993 CATTGC
CelII 2282 GCTCAGC GC^T[ACGT]AGC
4995 GCTGAGC
Eam1105I 1179 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5587 GACCTCGTGTC
EcoT22I 2170 ATGCAT ATGCA^T
5628 ATGCAT
HindIII 1012 AAGCTT A^AGCTT
6512 AAGCTT
KasI 1822 GGCGCC G^GCGCC
5652 GGCGCC
KspI 488 CCGCGG CCGC^GG
4122 CCGCGG
NarI 1822 GGCGCC GG^CGCC
5652 GGCGCC
NdeI 4347 CATATG CA^TATG
5190 CATATG
NsiI 2170 ATGCAT ATGCA^T
5628 ATGCAT
PaeR7I 2986 CTCGAG C^TCGAG
4566 CTCGAG
SacI 4998 GAGCTC GAGCT^C
5854 GAGCTC
SacII 488 CCGCGG CCGC^GG
4122 CCGCGG
SfoI 1822 GGCGCC GGC^GCC
5652 GGCGCC
SmaI 633 CCCGGG CCC^GGG
4000 CCCGGG
SphI 3018 GCATGC GCATG^C
5626 GCATGC
XhoI 2986 CTCGAG C^TCGAG
4566 CTCGAG
XmaCI 633 CCCGGG C^CCGGG
4000 CCCGGG
XmaI 633 CCCGGG C^CCGGG
4000 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI ApaI ApoI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BglI BglII
BmyI BpmI BsaHI BsaI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspLU11I BspMI BsrBI BsrI BssKI
BssSI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PshAI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmnI